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. 1978 Dec;75(12):6144–6148. doi: 10.1073/pnas.75.12.6144

Marker rescue and partial replication of bacteriophage T7 DNA.

K B Burck, R C Miller Jr
PMCID: PMC393135  PMID: 282630

Abstract

Experiments reported here show that some UV-irradiated wild-type T7 phage markers can be rescued efficiently by coinfection with T7 amber mutant phage in a permissive host. Other results show that the segments of a UV-irradiated genome that replicate efficiently are those that also are rescued efficiently during a marker rescue experiment. At higher doses, fewer markers are rescued efficiently and fewer segments of the genome replicate efficiently. The results clearly indicate that the probability of marker rescue is correlated with the ability of the DNA containing the marker to replicate. Sucrose density gradient analysis shows that UV irradiation does not produce double-strand scissions in T7 DNA at doses used here. Therefore, the partial replication and rescue of markers from the left end of the genome is not due simply to injection of only the left end of the T7 DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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