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. 2013 Jul 29;2:142. Originally published 2013 Jun 19. [Version 2] doi: 10.12688/f1000research.2-142.v2

Figure 2. Linearized pBC1 plasmid used for random insertional mutagenesis.

Figure 2.

The cleavage site of Kpn1 restriction enzyme, used for linearization of the vector is shown. APHVIII is under the control of combo promoters which consist of the promoter of the gene encoding the small subunit of Rubisco (RbcS2) and the gene encoding the heat shock protein 70A (Hsp70A). pBC1 is a phagemid and its F1 origin (F1 ori) and pUC origin (pUC ori) are shown. The size of the plasmid is 4763 bp.