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. 2013 Jul 29;2:142. Originally published 2013 Jun 19. [Version 2] doi: 10.12688/f1000research.2-142.v2

Figure 7. Transcript analyses of GUN4 and its neighboring genes.

Figure 7.

( A) A schematic map of a 21.497 kb genomic region spanning the GUN4 locus on chromosome 5. HYP1 and HYP2 are genes located upstream and downstream of the GUN4 gene, respectively coding for hypothetical proteins. Sulfocyanin ( SOXE) codes for a blue copper protein. The top black number denotes size of a gene (bp) while the bottom black number denotes distance between genes (bp). ( B) Semi-quantitative RT-PCR results. Lanes: 1, 3, 5, 7, 9 denote 4A+ cDNA products. Lanes 2, 4, 6, 8, 10 denote gun4 cDNA products. Primer sequences are shown in Table 4. All primers span an intron. Actin was used as a control. Actin genomic product size: 527 bp; Actin cDNA product size: 305 bp. HYP1 genomic product size 726 bp; HYP1 cDNA product size: 459 bp. GUN4 genomic product size: 942 bp; GUN4 cDNA product size 775 bp. HYP2 genomic product size: 797 bp; HYP2 cDNA product size: 184bp. SOXE genomic product size: 517 bp; SOXE cDNA product size: 119 bp.