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. 1978 Dec;75(12):6215–6219. doi: 10.1073/pnas.75.12.6215

Enhanced expression of β2-microglobulin and HLA antigens on human lymphoid cells by interferon

Iver Heron 1,*, Marianne Hokland 1, Kurt Berg 1
PMCID: PMC393150  PMID: 83651

Abstract

Mononuclear cells from the blood of healthy normal humans were kept in cultures under nonstimulating conditions for 16 hr in the presence or absence of human interferon. The relative quantities of HLA antigens and β2-microglobulin on the cultured cells were determined by quantitative immunofluorescence (fluorescence-activated cell sorter) and by the capacity of cells to absorb out cytotoxic antibodies against the relevant antigens. Interferons of different origin and purities enhanced the expression of HLA antigens and β2-microglobulins, whereas membrane immunoglobulins and antigens recognized by antiserum raised against human brain and T cells were the same on interferon-treated and control cells. Similar interferon effects were observed on an Epstein-Barrvirus-negative Burkitt lymphoma cell line. The enhanced expression of histocompatibility antigen subsequent to intereferon treatment was observed on B- and T-enriched lymphocyte populations and was found to be dose dependent with the optimum with “physiological” concentrations of interferon. Pretreatment of lymphocytes with interferon for 2 hr was found to be as effective as having interferon present during the total culture period. The interferon-induced enhancement of antigen expression on cells was dependent on active protein synthesis.

Keywords: histocompatibility antigens, membrane antigen expression

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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