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. 1978 Dec;75(12):6235–6239. doi: 10.1073/pnas.75.12.6235

Expression of Ia-like antigens on cultured human malignant melanoma cell lines.

R J Winchester, C Y Wang, A Gibofsky, H G Kunkel, K O Lloyd, L J Old
PMCID: PMC393154  PMID: 366616

Abstract

Human malignant melanoma cell lines were found to bear Ia-like cell surface determinants demonstrable by hetero- or alloantisera and by direct identification of the characteristic bimolecular glycoprotein complex. Immunoprecipitation confirmed the presence of Ia determinants on the bimolecular complex. The quantity of Ia molecules determined by these methods and by absorption experiments was relatively constant for each cell line. Among different lines, however, the amount of Ia antigens ranged from a level equal to that expressed by B-cell lines to a small fraction of this amount. This variation in level of Ia contrasted with the more uniform amount of beta2-microglobulin detected on the cell surface. The Ia alloantigen specificity DRw2 was the most frequently encountered specificity. Ia determinants were also found on the surface of an epidermoid carcinoma line, but not on various other cell lines of normal or neoplastic origin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  2. Carey T. E., Takahashi T., Resnick L. A., Oettgen H. F., Old L. J. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3278–3282. doi: 10.1073/pnas.73.9.3278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cullen S. E., Schwartz B. D. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci. J Immunol. 1976 Jul;117(1):136–142. [PubMed] [Google Scholar]
  4. Gahmberg C. G., Hakomori S. I. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem. 1973 Jun 25;248(12):4311–4317. [PubMed] [Google Scholar]
  5. Halper J., Fu S. M., Wang C. Y., Winchester R., Kunkel H. G. Patterns of expression of human "Ia-like" antigens during the terminal stages of B cell development. J Immunol. 1978 May;120(5):1480–1484. [PubMed] [Google Scholar]
  6. Hoffman T., Wang C. Y., Winchester R. J., Ferrarini M., Kunkel H. G. Human lymphocytes bearing "Ia-like" antigens; absence in patients with infantile agammaglobulinemia. J Immunol. 1977 Oct;119(4):1520–1524. [PubMed] [Google Scholar]
  7. Knowles D. N., 2nd, Winchester R. J., Kunkel H. G. A comparison of peroxidase- and fluorochrome-conjugated antisera for the demonstration of surface and intracellular antigens. Clin Immunol Immunopathol. 1977 May;7(3):410–425. doi: 10.1016/0090-1229(77)90076-9. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Marchalonis J. J., Cone R. E., Santer V. Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochem J. 1971 Oct;124(5):921–927. doi: 10.1042/bj1240921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  11. Stingl G., Katz S. I., Abelson L. D., Mann D. L. Immunofluorescent detection of human B cell alloantigens on S-Ig-positive lymphocytes and epidermal Langerhans cells. J Immunol. 1978 Feb;120(2):661–664. [PubMed] [Google Scholar]
  12. Stingl G., Katz S. I., Shevach E. M., Wolff-Schreiner E., Green I. Detection of Ia antigens on Langerhans cells in guinea pig skin. J Immunol. 1978 Feb;120(2):570–578. [PubMed] [Google Scholar]
  13. Winchester R. J., Ross G. D., Jarowski C. I., Wang C. Y., Halper J., Broxmeyer H. E. Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4012–4016. doi: 10.1073/pnas.74.9.4012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Winchester R. J., Wang C. Y., Halper J., Hoffman T. Studies with B-cell allo- and hetero-antisera: parallel reactivity and special properties. Scand J Immunol. 1976;5(6-7):745–757. doi: 10.1111/j.1365-3083.1976.tb03024.x. [DOI] [PubMed] [Google Scholar]
  15. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]

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