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. 1977 Jan;74(1):96–100. doi: 10.1073/pnas.74.1.96

Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

S Gillam, F Rottman, P Jahnke, M Smith
PMCID: PMC393204  PMID: 189317

Abstract

The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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