Abstract
A glucose binding fraction was obtained by sucrose gradient centrifugation and Sephadex G-200 chromatopgraphy from confluent normal cells and Rous sarcoma virus-transformed fibroblasts. It was more or less loosely bound to the membrane fraction, most strongly in sarcoma cells, and most loosely in slowly growing confluent fibroblasts. In an average of three determinations, the content of binding factor was increased 2.5-fold in transformed cells, and compared reasonably well to a nearly 4-fold increase in glucose uptake. The addition of 5 mug/ml of purified glucose binding factor to the overlaying fluid in the 100-mm plates increased 7-flod the low glucose uptake of starved fibroblasts. The stimulation was an additive increment to the known stimulation by calf serum.
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Selected References
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- Bose S. K., Zlotnick B. J. Growth-and density-dependent inhibition of deoxyglucose transport in Balb 3T3 cells and its absence in cells transformed by murine sarcoma virus. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2374–2378. doi: 10.1073/pnas.70.8.2374. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUMMEL J. P., DREYER W. J. Measurement of protein-binding phenomena by gel filtration. Biochim Biophys Acta. 1962 Oct 8;63:530–532. doi: 10.1016/0006-3002(62)90124-5. [DOI] [PubMed] [Google Scholar]
- Hatanaka M., Hanafusa H. Analysis of a functional change in membrane in the process of cell transformation by Rous sarcoma virus; alteration in the characteristics of sugar transport. Virology. 1970 Aug;41(4):647–652. doi: 10.1016/0042-6822(70)90429-0. [DOI] [PubMed] [Google Scholar]
- Hatanaka M. Transport of sugars in tumor cell membranes. Biochim Biophys Acta. 1974 Apr 29;355(1):77–104. doi: 10.1016/0304-419x(74)90008-0. [DOI] [PubMed] [Google Scholar]
- Kleitzien R. F., Perdue J. F. Sugar transport in chick embryo fibroblasts. 3. Evidence for post-transcriptional and post-translational regulation of transport following serum addition. J Biol Chem. 1974 Jun 10;249(11):3383–3387. [PubMed] [Google Scholar]
- Kletzien R. F., Perdue J. F. Sugar transport in chick embryo fibroblasts. II. Alterations in transport following transformation by a temperature-sensitive mutant of the Rous sarcoma virus. J Biol Chem. 1974 Jun 10;249(11):3375–3382. [PubMed] [Google Scholar]
- Lee S. G., Littau V., Lipmann F. The relation between sporulation and the induction of antibiotic synthesis and of amino acid uptake in Bacillus brevis. J Cell Biol. 1975 Aug;66(2):233–242. doi: 10.1083/jcb.66.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin G. S., Venuta S., Weber M., Rubin H. Temperature-dependent alterations in sugar transport in cells infected by a temperature-sensitive mutant of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2739–2741. doi: 10.1073/pnas.68.11.2739. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martineau R., Kohlbacher M., Shaw S. N., Amos H. Enhancement of hexose entry into chick fibroblasts by starvation: differential effect on galactose and glucose. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3407–3411. doi: 10.1073/pnas.69.11.3407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mizel S. B. Differential effect of cytochalasin B on the two possible modes of 2-deoxyglucose transport in HeLa cells. Nat New Biol. 1973 May 23;243(125):125–126. [PubMed] [Google Scholar]
- Sefton B. M., Rubin H. Stimulation of glucose transport in cultures of density-inhibited chick embryo cells. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3154–3157. doi: 10.1073/pnas.68.12.3154. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sun A. S., Poole B. Fractionation of rat fibroblasts in a zonal rotor by means of a viscosity barrier. Anal Biochem. 1975 Sep;68(1):260–273. doi: 10.1016/0003-2697(75)90704-6. [DOI] [PubMed] [Google Scholar]
- Yamada K. M., Yamada S. S., Pastan I. The major cell surface glycoprotein of chick embryo fibroblasts is an agglutinin. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3158–3162. doi: 10.1073/pnas.72.8.3158. [DOI] [PMC free article] [PubMed] [Google Scholar]