Table 1.
Primers and their sequences for aminoglycoside resistance encoding genes used in multiplex PCR.
| Genes | Primer sequences (5′-3′) | Size of PCR product (bp) | |
|---|---|---|---|
| aac(6′)-Ie-aph(2′′ )-Ia | F: CAGGAATTTATCGAAAATGGTAGAAAAG | R: CACAATCGACTAAAGAGTACCAATC | 369 |
| aph(2′′)-Ib | F: CTTGGACGCTGAGATATATGAGCAC | R: GTTTGTAGCAATTCAGAAACACCCTT | 867 |
| aph(2′′)-Ic | F: CCACAATGATAATGACTCAGTTCCC | R: CCACAGCTTCCGATAGCAAGAG | 444 |
| aph(2′′)-Id | F: GTGGTTTTTACAGGAATGCCATC | R: CCCTCTTCATACCAATCCATATAACC | 641 |
| aph(3′)-IIIa | F: GGCTAAAATGAGAATATCACCGG | R: CTTTAAAAAATCATACAGCTCGCG | 523 |