Abstract
Many nonsense suppressors of Saccharomyces cerevisiae cause growth inhibition on hypertonic media. Eight tyrosine-inserting UAA (ochre) suppressors, eight tyrosine-inserting UAG (amber) suppressors, a leucine-inserting UAG suppressor, and a serine-inserting recessive lethal UAG suppressor cause osmotic sensitivity, whereas a serine-inserting UAA suppressor does not cause sensitivity. Although the mechanism is not understood, the growth inhibition of specific suppressors on hypertonic media is correlated with their efficiencies of suppression. This heretofore unknown property of nonsense suppressors is useful for mitotic mapping, selecting tRNA mutants, selecting antisuppressors, and scoring nonsense suppressors.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brandriss M. C., Stewart J. W., Sherman F., Botstein D. Substitution of serine caused by a recessive lethal suppressor in yeast. J Mol Biol. 1976 Apr 15;102(3):467–476. doi: 10.1016/0022-2836(76)90328-4. [DOI] [PubMed] [Google Scholar]
- Capecchi M. R., Hughes S. H., Wahl G. M. Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro. Cell. 1975 Nov;6(3):269–277. doi: 10.1016/0092-8674(75)90178-6. [DOI] [PubMed] [Google Scholar]
- Cox B. S. A recessive lethal super-suppressor mutation in yeast and other psi phenomena. Heredity (Edinb) 1971 Apr;26(2):211–232. doi: 10.1038/hdy.1971.28. [DOI] [PubMed] [Google Scholar]
- DAVIES J., GILBERT W., GORINI L. STREPTOMYCIN, SUPPRESSION, AND THE CODE. Proc Natl Acad Sci U S A. 1964 May;51:883–890. doi: 10.1073/pnas.51.5.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gesteland R. F., Wolfner M., Grisafi P., Fink G., Botstein D., Roth J. R. Yeast suppressors of UAA and UAG nonsense codons work efficiently in vitro via tRNA. Cell. 1976 Mar;7(3):381–390. doi: 10.1016/0092-8674(76)90167-7. [DOI] [PubMed] [Google Scholar]
- Gilmore R. A., Stewart J. W., Sherman F. Amino acid replacements resulting from super-suppression of nonsense mutants of iso-1-cytochrome c from yeast. J Mol Biol. 1971 Oct 14;61(1):157–173. doi: 10.1016/0022-2836(71)90213-0. [DOI] [PubMed] [Google Scholar]
- Gorini L. Informational suppression. Annu Rev Genet. 1970;4:107–134. doi: 10.1146/annurev.ge.04.120170.000543. [DOI] [PubMed] [Google Scholar]
- HAWTHORNE D. C., FRIIS J. OSMOTIC-REMEDIAL MUTANTS. A NEW CLASSIFICATION FOR NUTRITIONAL MUTANTS IN YEAST. Genetics. 1964 Nov;50:829–839. doi: 10.1093/genetics/50.5.829. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hawthorne D. C., Leupold U. Suppressors in yeast. Curr Top Microbiol Immunol. 1974;64(0):1–47. doi: 10.1007/978-3-642-65848-8_1. [DOI] [PubMed] [Google Scholar]
- Liebman S. W., Sherman F. Inhibition of growth by amber suppressors in yeast. Genetics. 1976 Feb;82(2):233–249. doi: 10.1093/genetics/82.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liebman S. W., Sherman F., Stewart J. W. Isolation and characterization of amber suppressors in yeast. Genetics. 1976 Feb;82(2):251–272. doi: 10.1093/genetics/82.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liebman S. W., Stewart J. W., Sherman F. Serine substitutions caused by an ochre suppressor in yeast. J Mol Biol. 1975 Jun 5;94(4):595–610. doi: 10.1016/0022-2836(75)90324-1. [DOI] [PubMed] [Google Scholar]
- Martin C. E., DeBusk A. G. Temperature-sensitive, osmotic-remedial mutants of Neurospora crassa: osmotic pressure induced alterations of enzyme stability. Mol Gen Genet. 1975;136(1):31–40. doi: 10.1007/BF00275446. [DOI] [PubMed] [Google Scholar]
- McCready S. J., Cox B. S. Antisuppressors in yeast. Mol Gen Genet. 1973 Aug 28;124(4):305–320. doi: 10.1007/BF00267660. [DOI] [PubMed] [Google Scholar]
- Metzenberg R. L. Repair of multiple defects of a regulatory mutant of Neurospora by high osmotic pressure and by reversion. Arch Biochem Biophys. 1968 May;125(2):532–541. doi: 10.1016/0003-9861(68)90611-5. [DOI] [PubMed] [Google Scholar]
- SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
- Sherman F., Liebman S. W., Stewart J. W., Jackson M. Tyrosine substitutions resulting from suppression of amber mutants of iso-1-cytochrome c in yeast. J Mol Biol. 1973 Jun 25;78(1):157–168. doi: 10.1016/0022-2836(73)90435-x. [DOI] [PubMed] [Google Scholar]
- Sherman F., Stewart J. W., Parker J. H., Inhaber E., Shipman N. A., Putterman G. J., Gardisky R. L., Margoliash E. The mutational alteration of the primary structure of yeast iso-1-cytochrome c. J Biol Chem. 1968 Oct 25;243(20):5446–5456. [PubMed] [Google Scholar]