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. 1996 May 14;93(10):4633–4637. doi: 10.1073/pnas.93.10.4633

Regulation of the retinoblastoma protein-related protein p107 by G1 cyclin-associated kinases.

Z X Xiao 1, D Ginsberg 1, M Ewen 1, D M Livingston 1
PMCID: PMC39330  PMID: 8643455

Abstract

p107 is a retinoblastoma protein-related phosphoprotein that, when overproduced, displays a growth inhibitory function. It interacts with and modulates the activity of the transcription factor, E2F-4. In addition, p107 physically associates with cyclin E-CDK2 and cyclin A-CDK2 complexes in late G1 and at G1/S, respectively, an indication that cyclin-dependent kinase complexes may regulate, contribute to, and/or benefit from p107 function during the cell cycle. Our results show that p107 phosphorylation begins in mid G1 and proceeds through late G1 and S and that cyclin D-associated kinase(s) contributes to this process. In addition, E2F-4 binds selectively to hypophosphorylated p107, and G1 cyclin-dependent p107 phosphorylation leads to the dissociation of p107-E2F-4 complexes as well as inactivation of p107 G1 blocking function.

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Selected References

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