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. 1983 Jan;80(1):16–20. doi: 10.1073/pnas.80.1.16

DNA sequence preference of the progesterone receptor.

J G Compton, W T Schrader, B W O'Malley
PMCID: PMC393300  PMID: 6571991

Abstract

Highly purified hen oviduct progesterone receptor A subunit was tested for binding to several chicken gene DNAs. Sequence preference detected by nitrocellulose filter adsorption of [32P]DNA fragments obtained from recombinant plasmids revealed a marked retention of certain DNA fragments. About a 10-fold preference was seen for DNA fragments flanking the 5' end of the steroid-regulated genes ovalbumin and gene Y. No preference was seen with analogous DNA fragments from chicken beta-globin and alpha-actin genes. Restriction endonuclease mapping suggests the presence of multiple receptor interaction sites flanking the 5' terminus of the ovalbumin gene. One of these preferential binding sites was localized between -135 and -247 base pairs upstream from the start of transcription. This region contains an 18-base-pair A + T-rich sequence, a likely candidate for the binding site itself, because earlier studies had shown receptor A to have marked preference for A + T-rich DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnbaumer M. E., Schrader W. T., O'Malley B. W. Chemical cross-linking of chick oviduct progesterone-receptor subunits by using a reversible bifunctional cross-linking agent. Biochem J. 1979 Jul 1;181(1):201–213. doi: 10.1042/bj1810201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Colbert D. A., Knoll B. J., Woo S. L., Mace M. L., Tsai M. J., O'Malley B. W. Differential hormonal responsiveness of the ovalbumin gene and its pseudogenes in the chick oviduct. Biochemistry. 1980 Nov 25;19(24):5586–5592. doi: 10.1021/bi00565a020. [DOI] [PubMed] [Google Scholar]
  3. Compton J. G., Schrader W. T., O'Malley B. W. Selective binding of chicken progesterone receptor A subunit to a DNA fragment containing ovalbumin gene sequences. Biochem Biophys Res Commun. 1982 Mar 15;105(1):96–104. doi: 10.1016/s0006-291x(82)80015-6. [DOI] [PubMed] [Google Scholar]
  4. Coty W. A., Schrader W. T., O'Malley B. W. Purification and characterization of the chick oviduct progesterone receptor A subunit. J Steroid Biochem. 1979 Jan;10(1):1–12. doi: 10.1016/0022-4731(79)90134-1. [DOI] [PubMed] [Google Scholar]
  5. Day L. E., Hirst A. J., Lai E. C., Mace M., Jr, Woo S. L. 5' Domain and nucleotide sequence of an adult chicken chromosomal beta-globin gene. Biochemistry. 1981 Apr 14;20(8):2091–2098. doi: 10.1021/bi00511a005. [DOI] [PubMed] [Google Scholar]
  6. Dugaiczyk A., Woo S. L., Colbert D. A., Lai E. C., Mace M. L., Jr, O'Malley B. W. The ovalbumin gene: cloning and molecular organization of the entire natural gene. Proc Natl Acad Sci U S A. 1979 May;76(5):2253–2257. doi: 10.1073/pnas.76.5.2253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hughes M. R., Compton J. G., Schrader W. T., O'Malley B. W. Interaction of the chick oviduct progesterone receptor with deoxyribonucleic acid. Biochemistry. 1981 Apr 28;20(9):2481–2491. doi: 10.1021/bi00512a019. [DOI] [PubMed] [Google Scholar]
  8. Knoll B. J., Woo S. L., Beattie W., O'Malley B. W. Identification and sequence analysis of the 5' domain of the X and Y pseudo-ovalbumin genes. J Biol Chem. 1981 Aug 10;256(15):7949–7953. [PubMed] [Google Scholar]
  9. Kuhn R. W., Schrader W. T., Smith R. G., O'Malley B. W. Progesterone binding components of chick oviduct. X. Purification by affinity chromatography. J Biol Chem. 1975 Jun 10;250(11):4220–4228. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Mulvihill E. R., LePennec J. P., Chambon P. Chicken oviduct progesterone receptor: location of specific regions of high-affinity binding in cloned DNA fragments of hormone-responsive genes. Cell. 1982 Mar;28(3):621–632. doi: 10.1016/0092-8674(82)90217-3. [DOI] [PubMed] [Google Scholar]
  12. O'Farrell P. H., Kutter E., Nakanishi M. A restriction map of the bacteriophage T4 genome. Mol Gen Genet. 1980;179(2):421–435. doi: 10.1007/BF00425473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Malley B. W., Roop D. R., Lai E. C., Nordstrom J. L., Catterall J. F., Swaneck G. E., Colbert D. A., Tsai M. J., Dugaiczyk A., Woo S. L. The ovalbumin gene: organization, structure, transcription, and regulation. Recent Prog Horm Res. 1979;35:1–46. doi: 10.1016/b978-0-12-571135-7.50005-9. [DOI] [PubMed] [Google Scholar]
  14. Payvar F., Wrange O., Carlstedt-Duke J., Okret S., Gustafsson J. A., Yamamoto K. R. Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6628–6632. doi: 10.1073/pnas.78.11.6628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Royal A., Garapin A., Cami B., Perrin F., Mandel J. L., LeMeur M., Brégégègre F., Gannon F., LePennec J. P., Chambon P. The ovalbumin gene region: common features in the organisation of three genes expressed in chicken oviduct under hormonal control. Nature. 1979 May 10;279(5709):125–132. doi: 10.1038/279125a0. [DOI] [PubMed] [Google Scholar]
  16. Schrader W. T., O'Malley B. W. Progesterone-binding components of chick oviduct. IV. Characterization of purified subunits. J Biol Chem. 1972 Jan 10;247(1):51–59. [PubMed] [Google Scholar]
  17. Weigel N. L., Tash J. S., Means A. R., Schrader W. T., O'Malley B. W. Phosphorylation of hen progesterone receptor by cAMP dependent protein kinase. Biochem Biophys Res Commun. 1981 Sep 16;102(1):513–519. doi: 10.1016/0006-291x(81)91549-7. [DOI] [PubMed] [Google Scholar]
  18. Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]

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