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. 1983 Jan;80(1):165–168. doi: 10.1073/pnas.80.1.165

Proton-hydroxide permeability of liposomes.

D W Deamer, J W Nichols
PMCID: PMC393331  PMID: 6296864

Abstract

Liposome permeability to net proton-hydroxide flux was compared under two conditions that have produced extreme values in previous studies. In the first [Nichols, J.W. & Deamer, D.W. (1980) Proc. Natl. Acad. Sci. USA 77, 2038-2042] small pH gradients were permitted to decay near pH 7 and permeability coefficients in the range of 10(-4) cm/sec were obtained. In the second [Nozaki, Y. & Tanford, C. (1981) Proc. Natl. Acad. Sci. USA 78, 4324-4328] the decay rate of large pH gradients (approximately equal to 3 pH units) was measured and apparent permeability coefficients in the range of 10(-9) cm/sec were reported. We have repeated the conditions of the latter study and have found that the decay of large pH gradients produces a diffusion potential that limits net proton-hydroxide flux. The permeability coefficients calculated from the flux are therefore underestimated and do not reflect intrinsic proton permeability. With the same liposome preparations under conditions in which small gradients decay so that diffusion potentials are negligible, the measured permeability coefficient is near 10(-4) cm/sec, as reported earlier.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biegel C. M., Gould J. M. Kinetics of hydrogen ion diffusion across phospholipid vesicle membranes. Biochemistry. 1981 Jun 9;20(12):3474–3479. doi: 10.1021/bi00515a026. [DOI] [PubMed] [Google Scholar]
  2. Crandall E. D., Klocke R. A., Forster R. E. Hydroxyl ion movements across the human erythrocyte membrane. Measurement of rapid pH changes in red cell suspensions. J Gen Physiol. 1971 Jun;57(6):664–683. doi: 10.1085/jgp.57.6.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Deamer D. W., Prince R. C., Crofts A. R. The response of fluorescent amines to pH gradients across liposome membranes. Biochim Biophys Acta. 1972 Aug 9;274(2):323–335. doi: 10.1016/0005-2736(72)90180-0. [DOI] [PubMed] [Google Scholar]
  4. Fry D. W., White J. C., Goldman I. D. Rapid separation of low molecular weight solutes from liposomes without dilution. Anal Biochem. 1978 Oct 15;90(2):809–815. doi: 10.1016/0003-2697(78)90172-0. [DOI] [PubMed] [Google Scholar]
  5. Gutknecht J., Walter A. Transport of protons and hydrochloric acid through lipid bilayer membranes. Biochim Biophys Acta. 1981 Feb 20;641(1):183–188. doi: 10.1016/0005-2736(81)90582-4. [DOI] [PubMed] [Google Scholar]
  6. Meissner G., Young R. C. Proton permeability of sarcoplasmic reticulum vesicles. J Biol Chem. 1980 Jul 25;255(14):6814–6819. [PubMed] [Google Scholar]
  7. Mimms L. T., Zampighi G., Nozaki Y., Tanford C., Reynolds J. A. Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside. Biochemistry. 1981 Feb 17;20(4):833–840. doi: 10.1021/bi00507a028. [DOI] [PubMed] [Google Scholar]
  8. Mitchell P., Moyle J. Acid-base titration across the membrane system of rat-liver mitochondria. Catalysis by uncouplers. Biochem J. 1967 Aug;104(2):588–600. doi: 10.1042/bj1040588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nagle J. F., Morowitz H. J. Molecular mechanisms for proton transport in membranes. Proc Natl Acad Sci U S A. 1978 Jan;75(1):298–302. doi: 10.1073/pnas.75.1.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nichols J. W., Deamer D. W. Net proton-hydroxyl permeability of large unilamellar liposomes measured by an acid-base titration technique. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2038–2042. doi: 10.1073/pnas.77.4.2038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nichols J. W., Hill M. W., Bangham A. D., Deamer D. W. Measurement of net proton-hydroxyl permeability of large unilamellar liposomes with the fluorescent pH probe, 9-aminoacridine. Biochim Biophys Acta. 1980 Mar 13;596(3):393–403. doi: 10.1016/0005-2736(80)90126-1. [DOI] [PubMed] [Google Scholar]
  12. Nozaki Y., Tanford C. Proton and hydroxide ion permeability of phospholipid vesicles. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4324–4328. doi: 10.1073/pnas.78.7.4324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rossignol M., Thomas P., Grignon C. Proton permeability of liposomes from natural phospholipid mixtures. Biochim Biophys Acta. 1982 Jan 22;684(2):195–199. doi: 10.1016/0005-2736(82)90005-0. [DOI] [PubMed] [Google Scholar]

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