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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Jan;80(1):196–200. doi: 10.1073/pnas.80.1.196

Modulation of adenovirus transformation by thyroid hormone.

P B Fisher, D L Guernsey, I B Weinstein, I S Edelman
PMCID: PMC393338  PMID: 6296867

Abstract

We have examined the effect of triiodothyronine (T3) on de novo transformation of a cloned population of Fischer rat embryo fibroblasts (CREF) by a temperature-sensitive mutant (H5ts125) of type 5 adenovirus and on the expression of the transformed phenotype in these cells. When CREF cells were grown in medium lacking T3 before, during, and after infection with H5ts125, the yield of transformed foci was half that in the cultures supplemented with 1 nM T3. Selective addition or removal of T3 during various phases of the transformation process indicated that the hormone exerted its maximal effect within 72 hr after viral infection. T3 was also required for optimal growth in agar of two clones of CREF cells previously transformed by type 5 adenovirus, wt-3A and ts-7E. The tumor promoter 12-O-tetradecanoylphorbol 13-acetate could substitute for T3 in enhancing growth in agar of wt-3A but not of ts-7E, suggesting that the promoter and T3 modify anchorage-independent growth by different mechanisms. Normal CREF cells and both of the transformed CREF clones grew equally well in monolayer culture in medium containing or lacking T3. Both of the transformed CREF clones contained a lower number of nuclear T3 receptors than did CREF cells and they bound somewhat lower levels of phorbol dibutyrate. These results indicate that thyroid hormone modulates an early stage involved in adenovirus transformation and that it also enhances the expression of the transformed state in previously transformed cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIELSCHOWSKY F., BIELSCHOWSKY M., FLETCHER E. K. Investigations on the role of thyroxine in the development of hepatomas in hypophysectomized rats and pituitary dwarf mice. Br J Cancer. 1962 Jun;16:267–274. doi: 10.1038/bjc.1962.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eisenthal R., Cornish-Bowden A. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem J. 1974 Jun;139(3):715–720. doi: 10.1042/bj1390715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fisher P. B., Babiss L. E., Weinstein I. B., Ginsberg H. S. Analysis of type 5 adenovirus transformation with a cloned rat embryo cell line (CREF). Proc Natl Acad Sci U S A. 1982 Jun;79(11):3527–3531. doi: 10.1073/pnas.79.11.3527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fisher P. B., Bozzone J. H., Weinstein I. B. Tumor promoters and epidermal growth factor stimulate anchorage-independent growth of adenovirus-tranformed rat embryo cells. Cell. 1979 Nov;18(3):695–705. doi: 10.1016/0092-8674(79)90124-7. [DOI] [PubMed] [Google Scholar]
  6. Fisher P. B., Goldstein N. I., Weinstein I. B. Phenotypic properties and tumor promotor-induced alterations in rat embryo cells transformed by adenovirus. Cancer Res. 1979 Aug;39(8):3051–3057. [PubMed] [Google Scholar]
  7. Fisher P. B., Mufson R. A., Weinstein I. B., Little J. B. Epidermal growth factor, like tumor promoters, enhances viral and radiation-induced cell transformation. Carcinogenesis. 1981;2(3):183–187. doi: 10.1093/carcin/2.3.183. [DOI] [PubMed] [Google Scholar]
  8. Fisher P. B., Weinstein I. B., Eisenberg D., Ginsberg H. S. Interactions between adenovirus, a tumor promoter, and chemical carcinogens in transformation of rat embryo cell cultures. Proc Natl Acad Sci U S A. 1978 May;75(5):2311–2314. doi: 10.1073/pnas.75.5.2311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fong C. K., Ledinko N. Enhancement by estrogens of adenovirus or spontaneous transformation of hamster cell cultures. Cancer Res. 1970 Mar;30(3):889–892. [PubMed] [Google Scholar]
  10. Goodall C. M. Hepatic carcinogenesis in thyroidectomized rats: apparent blockade at the stage of initiation. Cancer Res. 1966 Sep;26(9):1880–1883. [PubMed] [Google Scholar]
  11. Guernsey D. L., Borek C., Edelman I. S. Crucial role of thyroid hormone in x-ray-induced neoplastic transformation in cell culture. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5708–5711. doi: 10.1073/pnas.78.9.5708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Guernsey D. L., Ong A., Borek C. Thyroid hormone modulation of X ray-induced in vitro neoplastic transformation. Nature. 1980 Dec 11;288(5791):591–592. doi: 10.1038/288591a0. [DOI] [PubMed] [Google Scholar]
  13. Horowitz A. D., Greenebaum E., Weinstein I. B. Identification of receptors for phorbol ester tumor promoters in intact mammalian cells and of an inhibitor of receptor binding in biologic fluids. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2315–2319. doi: 10.1073/pnas.78.4.2315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kennedy A. R., Mondal S., Heidelberger C., Little J. B. Enhancement of X-ray transformation by 12-O-tetradecanoyl-phorbol-13-acetate in a cloned line of C3H mouse embryo cells. Cancer Res. 1978 Feb;38(2):439–443. [PubMed] [Google Scholar]
  15. Kumar M. S., Chiang T., Deodhar S. D. Enhancing effect of thyroxine on tumor growth and metastases in syngeneic mouse tumor systems. Cancer Res. 1979 Sep;39(9):3515–3518. [PubMed] [Google Scholar]
  16. Martin R. G., Setlow V. P., Edwards C. A., Vembu D. The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells. Cell. 1979 Jul;17(3):635–643. doi: 10.1016/0092-8674(79)90271-x. [DOI] [PubMed] [Google Scholar]
  17. Milo G. E., Jr, Schaller J. P., Yohn D. S. Hormonal modification of adenovirus transformation of hamster cells in vitro. Cancer Res. 1972 Nov;32(11):2338–2347. [PubMed] [Google Scholar]
  18. Mishkin S., Morris H. P., Yalovsky M. A., Narasimha Murthy P. V. Increased survival of rats bearing Morris hepatoma 7800 after induction of hypothyroidism. Cancer Res. 1979 Jul;39(7 Pt 1):2371–2375. [PubMed] [Google Scholar]
  19. Mondal S., Brankow D. W., Heidelberger C. Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells. Cancer Res. 1976 Jul;36(7 Pt 1):2254–2260. [PubMed] [Google Scholar]
  20. Morishige W. K., Guernsey D. L. Triiodothyronine receptors in rat lung. Endocrinology. 1978 May;102(5):1628–1632. doi: 10.1210/endo-102-5-1628. [DOI] [PubMed] [Google Scholar]
  21. Samuels H. H., Stanley F., Casanova J. Depletion of L-3,5,3'-triiodothyronine and L-thyroxine in euthyroid calf serum for use in cell culture studies of the action of thyroid hormone. Endocrinology. 1979 Jul;105(1):80–85. doi: 10.1210/endo-105-1-80. [DOI] [PubMed] [Google Scholar]
  22. Samuels H. H., Tsai J. S. Thyroid hormone action. Demonstration of similar receptors in isolated nuclei of rat liver and cultured GH1 cells. J Clin Invest. 1974 Feb;53(2):656–659. doi: 10.1172/JCI107601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Seif R. Factors which disorganize microtubules or microfilaments increase the frequency of cell transformation by polyoma virus. J Virol. 1980 Nov;36(2):421–428. doi: 10.1128/jvi.36.2.421-428.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Vanderpool E. A., Roane P., Turner W. Enhancement of adenovirus transformation by pretreatment of rat embryo cells with estrogenic and androgenic hormones. Proc Soc Exp Biol Med. 1979 Apr;160(4):389–395. doi: 10.3181/00379727-160-40456. [DOI] [PubMed] [Google Scholar]
  25. Yamamoto N., zur Hausen H. Tumour promoter TPA enhances transformation of human leukocytes by Epstein-Barr virus. Nature. 1979 Jul 19;280(5719):244–245. doi: 10.1038/280244a0. [DOI] [PubMed] [Google Scholar]

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