Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Jan;80(1):293–297. doi: 10.1073/pnas.80.1.293

Induction of a B-lymphocyte receptor for a T cell-replacing factor by the crosslinking of surface IgD.

L J Yaffe, F D Finkelman
PMCID: PMC393359  PMID: 6600516

Abstract

The observation that anti-immunoglobulin antibodies and T cell-replacing factor (TRF) have a synergistic effect on the stimulation of B lymphocytes to differentiate into antibody-secreting cells suggested to us the possibility that the crosslinking of B-cell surface immunoglobulin by antigen or anti-immunoglobulin antibody might induce the expression of a B-cell receptor for TRF. In order to test this possibility we studied whether spleen cells from mice injected with 400-800 micrograms of anti-IgD antibody 1-3 days before sacrifice had an enhanced capacity to adsorb TRF activity from a partially purified culture supernatant of concanavalin A-stimulated mouse spleen cells. We found that spleen cells from euthymic or congenitally athymic mice injected 24-30 hr prior to sacrifice with either affinity-purified goat anti-mouse IgD antibody or a monoclonal allospecific anti-IgD antibody had greater than 100 times the TRF adsorptive capacity of spleen cells from control mice. In contrast, spleen cells from anti-IgD treated DBA/2Ha mice, which have been shown to have an immune defect associated with the lack of a TRF receptor, were unable to adsorb TRF activity from concanavalin A-stimulated helper supernatants. This suggests that the crosslinking of B-cell surface immunoglobulin may induce B lymphocytes to express a receptor or receptors for TRF and thus enhance B-cell responsiveness to this helper factor.

Full text

PDF
293

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chesnut R. W., Grey H. M. Studies on the capacity of B cells to serve as antigen-presenting cells. J Immunol. 1981 Mar;126(3):1075–1079. [PubMed] [Google Scholar]
  2. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  3. Delovitch T. L., Watson J., Battistella R., Harris J. F., Shaw J., Paetkau V. In vitro analysis of allogeneic lymphocyte interaction. V. Identification and characterization of two components of allogeneic effect factor, one of which displays H-2-restricted helper activity and the other, T cell-growth factor activity. J Exp Med. 1981 Jan 1;153(1):107–128. doi: 10.1084/jem.153.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finkelman F. D., Kessler S. W., Mushinski J. F., Potter M. IgD-secreting murine plasmacytomas: identification and partial characterization of two IgD myeloma proteins. J Immunol. 1981 Feb;126(2):680–687. [PubMed] [Google Scholar]
  5. Finkelman F. D., Scher I., Mond J. J., Kessler S., Kung J. T., Metcalf E. S. Polyclonal activation of the murine immune system by an antibody to IgD. II. Generation of polyclonal antibody production and cells with surface IgG. J Immunol. 1982 Aug;129(2):638–646. [PubMed] [Google Scholar]
  6. Finkelman F. D., Scher I., Mond J. J., Kung J. T., Metcalf E. S. Polyclonal activation of the murine immune system by an antibody to IgD. I. Increase in cell size and DNA synthesis. J Immunol. 1982 Aug;129(2):629–637. [PubMed] [Google Scholar]
  7. Gillis S., Watson J. Interleukin-2 dependent culture of cytolytic T cell lines. Immunol Rev. 1981;54:81–109. doi: 10.1111/j.1600-065x.1981.tb00435.x. [DOI] [PubMed] [Google Scholar]
  8. Hilfiker M. L., Moore R. N., Farrar J. J. Biologic properties of chromatographically separated murine thymoma-derived Interleukin 2 and colony-stimulating factor. J Immunol. 1981 Nov;127(5):1983–1987. [PubMed] [Google Scholar]
  9. Lake P., Clark E. A., Khorshidi M., Sunshine G. H. Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets. Eur J Immunol. 1979 Nov;9(11):875–886. doi: 10.1002/eji.1830091109. [DOI] [PubMed] [Google Scholar]
  10. Mond J. J., Seghal E., Kung J., Finkelman F. D. Increased expression of I-region-associated antigen (Ia) on B cells after cross-linking of surface immunoglobulin. J Immunol. 1981 Sep;127(3):881–888. [PubMed] [Google Scholar]
  11. Mosier D. E. Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters. J Exp Med. 1969 Feb 1;129(2):351–362. doi: 10.1084/jem.129.2.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Parker D. C., Fothergill J. J., Wadsworth D. C. B lymphocyte activation by insoluble anti-immunoglobulin: induction of immunoglobulin secretion by a T cell-dependent soluble factor. J Immunol. 1979 Aug;123(2):931–941. [PubMed] [Google Scholar]
  13. Parker D. C. Stimulation of mouse lymphocytes by insoluble anti-mouse immunoglobulin. Nature. 1975 Nov 27;258(5533):361–363. doi: 10.1038/258361a0. [DOI] [PubMed] [Google Scholar]
  14. Puré E., Isakson P. C., Takatsu K., Hamaoka T., Swain S. L., Dutton R. W., Dennert G., Uhr J. W., Vitetta E. S. Induction of B cell differentiation by T cell factors. I. Stimulation of IgM secretion by products of a T cell hybridoma and a T cell line. J Immunol. 1981 Nov;127(5):1953–1958. [PubMed] [Google Scholar]
  15. Puré E., Vitetta E. Induction of murine B cell proliferation by insolubilized anti-immunoglobulins. J Immunol. 1980 Sep;125(3):1240–1242. [PubMed] [Google Scholar]
  16. Schimpl A., Wecker E. A third signal in B cell activation given by TRF. Transplant Rev. 1975;23:176–188. doi: 10.1111/j.1600-065x.1975.tb00157.x. [DOI] [PubMed] [Google Scholar]
  17. Scribner D. J., Weiner H. L., Moorhead J. W. Anti-immunoglobulin stimulation of murine lymphocytes. V. Age-related decline in Fc receptor-mediated immunoregulation. J Immunol. 1978 Jul;121(1):377–382. [PubMed] [Google Scholar]
  18. Sell S. Studies on rabbit lymphocytes in vitro. V. The induction of blast transformation with sheep antisera to rabbit IgG subunits. J Exp Med. 1967 Feb 1;125(2):289–301. doi: 10.1084/jem.125.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sieckmann D. G. The use of anti-immunoglobulins to induce a signal for cell division in B lymphocytes via their membrane IgM and IgD. Immunol Rev. 1980;52:181–210. doi: 10.1111/j.1600-065x.1980.tb00335.x. [DOI] [PubMed] [Google Scholar]
  20. Takatsu K., Tominaga A., Hamaoka T. Antigen-induced T cell-replacing factor (TRF). I. Functional characterization of a TRF-producing helper T cell subset and genetic studies on TRF production. J Immunol. 1980 May;124(5):2414–2422. [PubMed] [Google Scholar]
  21. Tominaga A., Takatsu K., Hamaoka T. Antigen-induced T cell-replacing factor (TRF). II. X-linked gene control for the expression of TRF-acceptor site(s) on B lymphocytes and preparation of specific antiserum to that acceptor. J Immunol. 1980 May;124(5):2423–2429. [PubMed] [Google Scholar]
  22. Vitetta E., Krolick K. Allelic exclusion of IgD allotypes on murine B cells. J Immunol. 1980 Jun;124(6):2988–2990. [PubMed] [Google Scholar]
  23. Zitron I. M., Clevinger B. L. Regulation of murine B cells through surface immunoglobulin. I. Monoclonal anti-delta antibody that induces allotype-specific proliferation. J Exp Med. 1980 Nov 1;152(5):1135–1146. doi: 10.1084/jem.152.5.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES