Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Jan;80(2):422–424. doi: 10.1073/pnas.80.2.422

RNA-dependent RNA polymerases of plants

H Fraenkel-Conrat 1
PMCID: PMC393389  PMID: 16593271

Abstract

The existence of RNA-dependent RNA polymerases (EC 2.7.7.48) in plants has been definitely proven by their isolation in pure form from cucumber and tobacco in our laboratory and from cowpea at Wageningen. These enzymes are single-chain proteins of 100-130 kilodaltons. They show clear physical and biochemical differences characteristic for a given plant species, even when their amounts in the plants were greatly increased prior to isolation by infection with the same virus. The role of these enzymes in plant physiology remains unknown.

Keywords: RNA replication in eukaryotes, cucumber mosaic virus, template preference

Full text

PDF
422

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Astier-Manifacier S., Cornuet P. Purification et poids moléculaire d'une RNA polymérase RNA-dépendante de Brassica oleracea var. Botrytis. C R Acad Sci Hebd Seances Acad Sci D. 1978 Oct 30;287(11):1043–1046. [PubMed] [Google Scholar]
  2. Astier-Manifacier S., Cornuet P. RNA-dependent RNA polymerase in Chinese cabbage. Biochim Biophys Acta. 1971 Mar 25;232(3):484–493. doi: 10.1016/0005-2787(71)90602-2. [DOI] [PubMed] [Google Scholar]
  3. Baron M. H., Baltimore D. Antibodies against a synthetic peptide of the poliovirus replicase protein: reaction with native, virus-encoded proteins and inhibition of virus-specific polymerase activities in vitro. J Virol. 1982 Sep;43(3):969–978. doi: 10.1128/jvi.43.3.969-978.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bol J. F., Clerx-van Haaster C. M., Weening C. J. Host and virus specific RNA polymerases in alfalfa mosaic virus infected tobacco. Ann Microbiol (Paris) 1976 Jan;127A(1):183–192. [PubMed] [Google Scholar]
  5. Clerx C. M., Bol J. F. Properties of solubilized RNA-dependent RNA polymerase from alfalfa mosaic virus-infected and healthy tobacco plants. Virology. 1978 Dec;91(2):453–463. doi: 10.1016/0042-6822(78)90391-4. [DOI] [PubMed] [Google Scholar]
  6. Dasgupta A., Zabel P., Baltimore D. Dependence of the activity of the poliovirus replicase on the host cell protein. Cell. 1980 Feb;19(2):423–429. doi: 10.1016/0092-8674(80)90516-4. [DOI] [PubMed] [Google Scholar]
  7. Duda C. T., Zaitlin M., Siegel A. In vitro synthesis of double-stranded RNA by an enzyme system isolated from tobacco leaves. Biochim Biophys Acta. 1973 Aug 10;319(1):62–71. doi: 10.1016/0005-2787(73)90041-5. [DOI] [PubMed] [Google Scholar]
  8. Flanegan J. B., Baltimore D. Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A). Proc Natl Acad Sci U S A. 1977 Sep;74(9):3677–3680. doi: 10.1073/pnas.74.9.3677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fraenkel-Conrat H. RNA polymerase from tobacco necrosis virus infected and uninfected tobacco. Purification of the membrane-associated enzyme. Virology. 1976 Jul 1;72(1):23–32. doi: 10.1016/0042-6822(76)90308-1. [DOI] [PubMed] [Google Scholar]
  10. Hardy S. F., German T. L., Loesch-Fries L. S., Hall T. C. Highly active template-specific RNA-dependent RNA polymerase from barley leaves infected with brome mosaic virus. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4956–4960. doi: 10.1073/pnas.76.10.4956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ikegami M., Fraenkel-Conrat H. Characterization of double-stranded ribonucleic acid in tobacco leaves. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3637–3640. doi: 10.1073/pnas.76.8.3637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ikegami M., Fraenkel-Conrat H. Characterization of the RNA-dependent RNA polymerase of tobacco leaves. J Biol Chem. 1979 Jan 10;254(1):149–154. [PubMed] [Google Scholar]
  13. Ikegami M., Fraenkel-Conrat H. RNA-dependent RNA polymerase of tobacco plants. Proc Natl Acad Sci U S A. 1978 May;75(5):2122–2124. doi: 10.1073/pnas.75.5.2122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kumarasamy R., Symons R. H. Extensive purification of the cucumber mosaic virus-induced RNA replicase. Virology. 1979 Jul 30;96(2):622–632. doi: 10.1016/0042-6822(79)90118-1. [DOI] [PubMed] [Google Scholar]
  15. Landers T. A., Blumenthal T., Weber K. Function and structure in ribonucleic acid phage Q beta ribonucleic acid replicase. The roles of the different subunits in transcription of synthetic templates. J Biol Chem. 1974 Sep 25;249(18):5801–5808. [PubMed] [Google Scholar]
  16. May J. T., Symons R. H. Specificity of the cucumber mosaic virus-induced RNA polymerase for RNA and polynucleotide templates. Virology. 1971 Jun;44(3):517–526. doi: 10.1016/0042-6822(71)90365-5. [DOI] [PubMed] [Google Scholar]
  17. Romaine C. P., Zaitlin M. RNA-dependent RNA polymerases in uninfected and tobacco mosaic virus-infected tabacco leaves: viral induced stimulation of a host polymerase activity. Virology. 1978 May 1;86(1):241–253. doi: 10.1016/0042-6822(78)90024-7. [DOI] [PubMed] [Google Scholar]
  18. Stussi-Garaud C., Lemius J., Fraenkel-Conrat H. RNA polymerase from tobacco necrosis virus-infected and uninfected tobacco. II. Properties of the bound and soluble polymerases and the nature of their products. Virology. 1977 Sep;81(2):224–236. doi: 10.1016/0042-6822(77)90139-8. [DOI] [PubMed] [Google Scholar]
  19. Sänger H. L. Functions of the two particles of tobacco rattle virus. J Virol. 1969 Mar;3(3):304–312. doi: 10.1128/jvi.3.3.304-312.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Takanami Y., Fraenkel-Conrat H. Comparative studies on ribonucleic acid dependent RNA polymerases in cucumber mosaic virus infected cucumber and tobacco and uninfected tobacco plants. Biochemistry. 1982 Jun 22;21(13):3161–3167. doi: 10.1021/bi00256a020. [DOI] [PubMed] [Google Scholar]
  21. Van Dyke T. A., Flanegan J. B. Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase. J Virol. 1980 Sep;35(3):732–740. doi: 10.1128/jvi.35.3.732-740.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES