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. 1983 Jan;80(2):477–481. doi: 10.1073/pnas.80.2.477

Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.

D J Jolly, H Okayama, P Berg, A C Esty, D Filpula, P Bohlen, G G Johnson, J E Shively, T Hunkapillar, T Friedmann
PMCID: PMC393401  PMID: 6300847

Abstract

We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.

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Selected References

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