Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Feb;80(3):712–715. doi: 10.1073/pnas.80.3.712

Sterol synergism in yeast.

M Ramgopal, K Bloch
PMCID: PMC393449  PMID: 6338497

Abstract

Sterol synergism as previously observed [Dahl, C.E., Dahl, J.S. & Bloch, K. (1980) Biochemistry 19, 1462-1467] and defined as a greater-than-additive growth response to pairs of sterols by Mycoplasma capricolum [Dahl, J.S., Dahl, C.E. & Bloch, K. (1981) J. Biol. Chem. 256, 87-91] is now demonstrated in the yeast mutant GL7, which is auxotrophic for sterol and unsaturated fatty acid. Mutant cells growing poorly when provided with cholesterol and oleic acid respond to ergosterol supplements (ergosterol-to-cholesterol ratio, 1:3) by a pronounced increase in growth rates and cell yields. Stigmasterol also elicits a significant synergistic effect, and 7-dehydrocholesterol, a smaller one. Evidence for a metabolic role of ergosterol in yeast membranes is presented. Cells raised on a 1:3 mixture of ergosterol to cholesterol up to midlogarithmic phase subsequently incorporate [1-14C]oleic acid at significantly faster rates into phospholipids than do cells grown on cholesterol alone.

Full text

PDF
712

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDREASEN A. A., STIER T. J. Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. J Cell Physiol. 1954 Jun;43(3):271–281. doi: 10.1002/jcp.1030430303. [DOI] [PubMed] [Google Scholar]
  2. Ariga N., Katsuki H. Sterol-content lowering action of o-chlorobenzylchloride in yeast. J Biochem. 1980 Jul;88(1):97–102. [PubMed] [Google Scholar]
  3. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  4. Buttke T. M., Bloch K. Comparative responses of the yeast mutant strain GL7 to lanosterol, cycloartenol, and cyclolaudenol. Biochem Biophys Res Commun. 1980 Jan 15;92(1):229–236. doi: 10.1016/0006-291x(80)91543-0. [DOI] [PubMed] [Google Scholar]
  5. Buttke T. M., Bloch K. Utilization and metabolism of methyl-sterol derivatives in the yeast mutant strain GL7. Biochemistry. 1981 May 26;20(11):3267–3272. doi: 10.1021/bi00514a044. [DOI] [PubMed] [Google Scholar]
  6. Buttke T. M., Jones S. D., Bloch K. Effect of sterol side chains on growth and membrane fatty acid composition of Saccharomyces cerevisiae. J Bacteriol. 1980 Oct;144(1):124–130. doi: 10.1128/jb.144.1.124-130.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DULANEY E. L., STAPLEY E. O., SIMPF K. Studies on ergosterol production by yeasts. Appl Microbiol. 1954 Nov;2(6):371–379. doi: 10.1128/am.2.6.371-379.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dahl C. E., Dahl J. S., Bloch K. Effect of alkyl-substituted precursors of cholesterol on artificial and natural membranes and on the viability of Mycoplasma capricolum. Biochemistry. 1980 Apr 1;19(7):1462–1467. doi: 10.1021/bi00548a031. [DOI] [PubMed] [Google Scholar]
  9. Dahl J. S., Dahl C. E., Bloch K. Effect of cholesterol on macromolecular synthesis and fatty acid uptake by Mycoplasma capricolum. J Biol Chem. 1981 Jan 10;256(1):87–91. [PubMed] [Google Scholar]
  10. Dahl J. S., Dahl C. E., Bloch K. Sterols in membranes: growth characteristics and membrane properties of Mycoplasma capricolum cultured on cholesterol and lanosterol. Biochemistry. 1980 Apr 1;19(7):1467–1472. doi: 10.1021/bi00548a032. [DOI] [PubMed] [Google Scholar]
  11. Dorling P. R., Le Page R. N. A rapid high yield method for the preparation of rat liver cell plasma membranes. Biochim Biophys Acta. 1973 Aug 9;318(1):33–40. doi: 10.1016/0005-2736(73)90333-7. [DOI] [PubMed] [Google Scholar]
  12. Durán A., Bowers B., Cabib E. Chitin synthetase zymogen is attached to the yeast plasma membrane. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3952–3955. doi: 10.1073/pnas.72.10.3952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gollub E. G., Liu K. P., Dayan J., Adlersberg M., Sprinson D. B. Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene. J Biol Chem. 1977 May 10;252(9):2846–2854. [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Merkel G. J., Naider F., Becker J. M. Amino acid uptake by Saccharomyces cerevisiae plasma membrane vesicles. Biochim Biophys Acta. 1980;595(1):109–120. doi: 10.1016/0005-2736(80)90252-7. [DOI] [PubMed] [Google Scholar]
  16. Nelson G. J. Composition of neutral lipids from erythrocytes of common mammals. J Lipid Res. 1967 Jul;8(4):374–379. [PubMed] [Google Scholar]
  17. Nes W. R., Sekula B. C., Nes W. D., Adler J. H. The functional importance of structural features of ergosterol in yeast. J Biol Chem. 1978 Sep 10;253(17):6218–6225. [PubMed] [Google Scholar]
  18. Nurminen T., Taskinen L., Suomalainen H. Distribution of membranes, especially of plasma-membrane fragments, during zonal centrifugations of homogenates from glucose-repressed Saccharomyces Cerevisiae. Biochem J. 1976 Mar 15;154(3):751–763. doi: 10.1042/bj1540751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Odriozola J. M., Waitzkin E., Smith T. L., Bloch K. Sterol requirement of Mycoplasma capricolum. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4107–4109. doi: 10.1073/pnas.75.9.4107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rodriguez R. J., Taylor F. R., Parks L. W. A requirement for ergosterol to permit growth of yeast sterol auxotrophs on cholestanol. Biochem Biophys Res Commun. 1982 May 31;106(2):435–441. doi: 10.1016/0006-291x(82)91129-9. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES