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. 1983 Feb;80(3):830–834. doi: 10.1073/pnas.80.3.830

Anti-idiotypic antibodies to anti-HLA receptors induced by pregnancy

N Suciu-Foca *, E Reed *, C Rohowsky *, P Kung *,†, D W King *
PMCID: PMC393474  PMID: 6600840

Abstract

We have previously shown that lymphoblasts alloactivated in vitro acquire the capacity of stimulating the autologous mixed lymphocyte response. This response is anti-idiotypic in nature because lymphocytes so primed display accelerated memory responses only when restimulated by autologous lymphoblasts that have been alloactivated against the same HLA-DR antigen. Based on this observation we have postulated that the absence of HLA antibodies in alloimmunized human subjects may be due to the development of autoantibodies that react with the anti-HLA receptors expressed by primed lymphocytes or by anti-HLA antibodies or both. This hypothesis has been confirmed in the present investigations which show that sera from parous women react with autologous T lymphoblasts primed in 5-day mixed lymphocyte culture against their husband—i.e., with lymphoblasts expressing receptors for the immunizing donor. Anti-HLA receptors expressed by T and B lymphocytes seem to share serologic determinants because sera that bind to autologous alloactivated lymphoblasts are also capable of inhibiting the anti-HLA activity of autologous and homologous sera. Auto-anti-idiotypic antibodies inhibit the autologous mixed lymphocyte response to autologous alloactivated lymphoblasts, a phenomenon whose in vivo correlate may reside in autoinhibition of anti-HLA antibody formation and of allograft immunity. Because auto-anti-idiotypic antibodies were found in sera from all parous women tested, the hypothesis that nonresponsiveness to alloantigens exists as a state per se is not likely. The passive transfer of antireceptor (idiotype) immunity by use of antibodies from pregnant women's sera may provide a powerful tool for specific suppression of allograft rejection.

Keywords: T-cell receptors, allogeneic reactivity

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bellgrau D., Wilson D. B. Immunological studies of T-cell receptors. II. Limited polymorphism of idiotypic determinants on T-cell receptors specific for major histocompatibility complex alloantigens. J Exp Med. 1979 Jan 1;149(1):234–243. doi: 10.1084/jem.149.1.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Binz H., Wigzell H. Antigen-binding, idiotypic receptors from T lymphocytes: an analysis of their biochemistry, genetics, and use as immunogens to produce specific immune tolerance. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):275–284. doi: 10.1101/sqb.1977.041.01.033. [DOI] [PubMed] [Google Scholar]
  3. Goyert S. M., Shively J. E., Silver J. Biochemical characterization of a second family of human Ia molecules, HLA-DS, equivalent to murine I-A subregion molecules. J Exp Med. 1982 Aug 1;156(2):550–566. doi: 10.1084/jem.156.2.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hoffman R. A., Kung P. C., Hansen W. P., Goldstein G. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4914–4917. doi: 10.1073/pnas.77.8.4914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Indiveri F., Ng A. K., Russo C., Quaranta V., Pellegrino M. A., Ferrone S. Isolation of Ia-like antigen-bearing cells from human peripheral lymphocytes through the use of a monoclonal antibody to framework determinants of Ia-like antigens. J Immunol Methods. 1980;39(4):343–354. doi: 10.1016/0022-1759(80)90234-3. [DOI] [PubMed] [Google Scholar]
  6. Infante A. J., Infante P. D., Gillis S., Fathman C. G. Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones. J Exp Med. 1982 Apr 1;155(4):1100–1107. doi: 10.1084/jem.155.4.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lafferty K. J., Talmage D. W. Theory of allogeneic reactivity and its relevance to transplantation biology. Transplant Proc. 1976 Sep;8(3):349–353. [PubMed] [Google Scholar]
  8. Opelz G., Terasaki P. I. Studies on the strength of HLA antigens in related donor kidney transplants. Transplantation. 1977 Aug;24(2):106–111. doi: 10.1097/00007890-197708000-00003. [DOI] [PubMed] [Google Scholar]
  9. Rajewsky K., Eichmann K. Antigen receptors of T helper cells. Contemp Top Immunobiol. 1977;7:69–112. doi: 10.1007/978-1-4684-3054-7_3. [DOI] [PubMed] [Google Scholar]
  10. Salvatierra O., Jr, Iwaki Y., Vincenti F., Amend W., Terasaki P., Garovoy M., Duca R., Hopper S., Feduska N. Update of the University of California at San Francisco experience with donor-specific blood transfusions. Transplant Proc. 1982 Jun;14(2):363–366. [PubMed] [Google Scholar]
  11. Suciu-Foca N., Buda J., McManus J., Thiem T., Reemtsma K. Impaired responsiveness of lymphocytes and serum-inhibitory factors in patients with cancer. Cancer Res. 1973 Oct;33(10):2373–2377. [PubMed] [Google Scholar]
  12. Suciu-Foca N., Reed E., Khan R., Coburn C., Lewison A., Hassanali R., Rohowsky C., Susinno E., Reemtsma K. HLA antigens in Asian Indians: HLA-D-DR relationships. Hum Immunol. 1981 Nov;3(3):261–270. doi: 10.1016/0198-8859(81)90022-7. [DOI] [PubMed] [Google Scholar]
  13. Suciu-Foca N., Rohowsky C., Kung P., King D. W. Idiotype-like determinants on human T lymphocytes alloactivated in mixed lymphocyte culture. J Exp Med. 1982 Jul 1;156(1):283–288. doi: 10.1084/jem.156.1.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wylie D. E., Sherman L. A., Klinman N. R. Participation of the major histocompatibility complex in antibody recognition of viral antigens expressed on infected cells. J Exp Med. 1982 Feb 1;155(2):403–414. doi: 10.1084/jem.155.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]

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