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. 1983 Mar;80(5):1212–1216. doi: 10.1073/pnas.80.5.1212

Stability of erythrocyte ghosts: a gamma-ray perturbed angular correlation study.

C A Kruse, G W Tin, J D Baldeschwieler
PMCID: PMC393564  PMID: 6572379

Abstract

The structural integrity of erythrocyte ghosts made by the preswell and slow-dialysis techniques has been studied in vitro by use of gamma-ray perturbed angular correlation (PAC) techniques and also by standard in vitro leakage methods employing sequestered labeled markers. Complexes of 111In3+ and nitrilotriacetate were encapsulated in ghosts made from human, rabbit, rat, and mouse erythrocytes, and their leakage was monitored by both methods. In addition, 125I-labeled bovine serum albumin was encapsulated, and ghost integrity was monitored by conventional leakage measurements. With the PAC technique the percentage of material released from human ghosts was determined quantitatively, and the results were equivalent to those obtained by the conventional method. In addition, at various times after intravenous injection, tissue distribution of the ghosts in the mouse was studied. The percent injected dose per gram of tissue of the labeled surface proteins of erythrocyte ghosts in circulation approximated that of the entrapped labeled albumin. This suggests that the ghost membrane and contents are strongly associated in vivo. Large 125I-labeled bovine serum albumin molecules and small 111In3+-nitrilotriacetate complexes were delivered in high quantitites to the lung initially, and to the liver and spleen. Because erythrocyte ghosts have the ability to entrap a wide range of substances and deliver them to specific organs, ghosts may be preferable to other drug carriers or drug therapy for treatment of certain disorders.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fiddler M. B., Hudson L. D., White J. G., Desnick R. J. Enzyme therapy XIV. Comparison of methods for enzyme entrapment in human erythrocytes. J Lab Clin Med. 1980 Aug;96(2):307–317. [PubMed] [Google Scholar]
  2. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  3. Hubbard A. R., Sprandel U., Chalmers R. A. Organic-acid transport in resealed haemoglobin-containing human erythrocyte 'ghosts'. Biochem J. 1980 Sep 15;190(3):653–658. doi: 10.1042/bj1900653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hwang K. J., Mauk M. R. Fate of lipid vesicles in vivo: a gamma-ray perturbed angular correlation study. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4991–4995. doi: 10.1073/pnas.74.11.4991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Isomaa B., Paatero G. Shape and volume changes in rat erythrocytes induced by surface-active alkyltrimethylammonium salts and sodium dodecyl sulphate. Biochim Biophys Acta. 1981 Oct 2;647(2):211–222. doi: 10.1016/0005-2736(81)90248-0. [DOI] [PubMed] [Google Scholar]
  6. Jausel-Hüsken S., Deuticke B. General and transport properties of hypotonic and isotonic preparations of resealed erythrocyte ghosts. J Membr Biol. 1981;63(1-2):61–70. doi: 10.1007/BF01969446. [DOI] [PubMed] [Google Scholar]
  7. Kitao T., Hattori K. Erythrocyte entrapment of daunomycin by amphotericin B without hemolysis. Cancer Res. 1980 Apr;40(4):1351–1353. [PubMed] [Google Scholar]
  8. Kruse C. A., Popják G. Fractionation of arginase-loaded erythrocyte ghosts with Percoll density gradients. Biochem Biophys Res Commun. 1981 Sep 16;102(1):258–264. doi: 10.1016/0006-291x(81)91515-1. [DOI] [PubMed] [Google Scholar]
  9. Kruse C. A., Spector E. B., Cederbaum S. D., Wisnieski B. J., Popják G. Microinjection of arginase into enzyme-deficient cells with the isolated glycoproteins of Sendai virus as fusogen. Biochim Biophys Acta. 1981 Jul 20;645(2):339–345. doi: 10.1016/0005-2736(81)90205-4. [DOI] [PubMed] [Google Scholar]
  10. Mauk M. R., Gamble R. C., Baldeschwieler J. D. Vesicle targeting: timed release and specificity for leukocytes in mice by subcutaneous injection. Science. 1980 Jan 18;207(4428):309–311. doi: 10.1126/science.7350660. [DOI] [PubMed] [Google Scholar]
  11. Mauk M. R., Gamble R. C. Preparation of lipid vesicles containing high levels of entrapped radioactive cations. Anal Biochem. 1979 Apr 15;94(2):302–307. doi: 10.1016/0003-2697(79)90364-6. [DOI] [PubMed] [Google Scholar]
  12. Mauk M. R., Gamble R. C. Stability of lipid vesicles in tissues of the mouse: a gamma-ray perturbed angular correlation study. Proc Natl Acad Sci U S A. 1979 Feb;76(2):765–769. doi: 10.1073/pnas.76.2.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meares C. F., Sundberg M. W., Baldeschwieler J. D. Perturbed angular correlation study of a haptenic molecule. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3718–3722. doi: 10.1073/pnas.69.12.3718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Romero P. J. Active calcium transport in red cell ghosts resealed in dextran solutions. Biochim Biophys Acta. 1981 Dec 7;649(2):404–418. doi: 10.1016/0005-2736(81)90430-2. [DOI] [PubMed] [Google Scholar]
  15. Updike S. J., Wakamiya R. T., Lightfoot E. N., Jr Asparaginase entrapped in red blood cells: action and survival. Science. 1976 Aug 20;193(4254):681–683. doi: 10.1126/science.821145. [DOI] [PubMed] [Google Scholar]
  16. Zimmermann U., Pilwat G., Esser B. The effect of encapsulation in red blood cells on the distribution of methotrexate in mice. J Clin Chem Clin Biochem. 1978 Feb;16(2):135–144. doi: 10.1515/cclm.1978.16.2.135. [DOI] [PubMed] [Google Scholar]

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