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Colon organoids were created from 500 crypt bottoms and plated in triplicate in 24-well plates. Crypt bottoms were isolated on separate days from 5 of each, age, gender and littermate matched Apc+/+ Kcnq1−/− and Apc+/+Kcnq1+/+ mice (one matched pair per day). Organoids were examined daily and counted at 5 days post-plating. * P <0.05.
