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. 1983 Mar;80(5):1401–1405. doi: 10.1073/pnas.80.5.1401

Indirect stimulation of genetic recombination

Efim I Golub 1, K Brooks Low 1
PMCID: PMC393605  PMID: 6219392

Abstract

Recombination between lacZ alleles in crosses of λlacZ1- × λlacZ2- and F-lacZ1- × λlacZ2- in Escherichia coli (λ) can be stimulated manyfold by UV irradiation of one of the λlacZ phages [Porter, R. D., McLaughlin, T. & Low, B. (1979) Cold Spring Harbor Symp. Quant. Biol. 43, 1043-1048]. Analogous stimulation has now been observed by coinfection of the cells by UV-irradiated λ phage which carries no lac region. This indirect stimulation is not dependent on induction of the SOS system. The bacterial uvr system can effectively remove the damages on the λ DNA which cause the indirect stimulation. Among a number of mutations tested, only ssb-1 was found to cause a drastic decrease in the indirect stimulation. Indirect stimulation was caused only by using phage that had a region of homology with the recombining phage. The homologous region can be separated from the recombining region by an extended nonhomologous region (>7.9 × 103 base pairs). This implies that damages to the DNA molecule, which stimulate recombination, can be located very far from the recombining region of the molecule.

Keywords: DNA damage, UV irradiation, λ crosses, uvrA gene, ssb gene

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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