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. 1983 Mar;80(5):1440–1444. doi: 10.1073/pnas.80.5.1440

Primed lymphocyte testing specificity of alloreactive lymphocyte clones for HLA-B locus determinants.

A Zeevi, C Scheffel, K Annen, R J Duquesnoy
PMCID: PMC393613  PMID: 6187013

Abstract

We have generated two alloreactive T-lymphocyte clones that recognize the products of the HLA-B locus. The primed lymphocyte testing (PLT) reactivity of clone HJ21 was monospecific for the HLA-B antigen Bw49, a subtype specificity of HLA-Bw21. The PLT specificity of clone RD3 was strongly associated with HLA-Bw21. Several monoclonal antibodies against class I HLA molecules inhibited the PLT responses of these clones whereas class II monoclonal antibodies had no effect. These observations provide experimental evidence that class I products of the HLA-B locus may directly be involved in lymphocyte alloactivation.

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Selected References

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  1. Bijnen A. B., Schreuder I., Volkers W. S., Parlevliet J., van Rood J. J. The lymphocyte activating influence of the HLA-A region. J Immunogenet. 1977 Feb;4(1):1–5. doi: 10.1111/j.1744-313x.1977.tb00608.x. [DOI] [PubMed] [Google Scholar]
  2. Brodsky F. M., Bodmer W. F., Parham P. Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979 Jul;9(7):536–545. doi: 10.1002/eji.1830090709. [DOI] [PubMed] [Google Scholar]
  3. Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979;47:3–61. doi: 10.1111/j.1600-065x.1979.tb00288.x. [DOI] [PubMed] [Google Scholar]
  4. Brodsky F. M., Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J Immunol. 1982 Jan;128(1):129–135. [PubMed] [Google Scholar]
  5. Duquesnoy R. J. Immunogenetic aspects of the relationship between HLA-DR and the MB and MT system. Transplant Proc. 1981 Dec;13(4):1804–1807. [PubMed] [Google Scholar]
  6. Duquesnoy R. J., Marrari M., Annen K. Identification of an HLA-DR-associated system of B-cell alloantigens. Transplant Proc. 1979 Dec;11(4):1757–1760. [PubMed] [Google Scholar]
  7. Duquesnoy R. J., Zeevi A., Marrari M., Halim K. Immunogenetic analysis of the HLA-D region: serological and cellular detection of the MB system. Clin Immunol Immunopathol. 1982 May;23(2):254–268. doi: 10.1016/0090-1229(82)90112-x. [DOI] [PubMed] [Google Scholar]
  8. Eijsvoogel V. P., van Rood J. J., Du Toit E. D., Schellekens P. T. [Position of a locus determining mixed lymphocyte reaction distinct from the known HL-A loci]. Eur J Immunol. 1972 Oct;2(5):413–418. doi: 10.1002/eji.1830020506. [DOI] [PubMed] [Google Scholar]
  9. Goulmy E., Termijtelen A., Bradley B. A., van Rood J. J. HLA restriction of non-HLA--A, --B, --C and --D cell mediated lympholysis (CML). Tissue Antigens. 1976 Nov;8(5):317–326. doi: 10.1111/j.1399-0039.1976.tb00583.x. [DOI] [PubMed] [Google Scholar]
  10. Goyert S. M., Silver J. Isolation of I-A subregion-like molecules from subhuman primates and man. Nature. 1981 Nov 19;294(5838):266–268. doi: 10.1038/294266a0. [DOI] [PubMed] [Google Scholar]
  11. Grumet F. C., Colombe B. W., Goodfellow P. Bw21 partition and crossreactivity of the components. Tissue Antigens. 1978 Jan;11(1):39–44. doi: 10.1111/j.1399-0039.1978.tb01220.x. [DOI] [PubMed] [Google Scholar]
  12. Grunnet N., Kristensen T., Kissmeyer-Nielsen F. Cell mediated lympholysis in man. Patterns of killing power in relation to the HL-A system. Tissue Antigens. 1975 Oct;6(4):205–220. doi: 10.1111/j.1399-0039.1975.tb00635.x. [DOI] [PubMed] [Google Scholar]
  13. Hartzman R. J., Pappas F., Romano P. J., Johnson A. H., Ward F. E., Amos D. B. Dissociation of HLA-D and HLA-DR using primed LD typing. Transplant Proc. 1978 Dec;10(4):809–812. [PubMed] [Google Scholar]
  14. Inouye H., Hank J. A., Chardonnens X., Segall M., Alter B. J., Bach F. H. Cloned primed-lymphocyte-test reagents in the dissection of HLA-D. J Exp Med. 1980 Aug 1;152(2 Pt 2):143s–155s. [PubMed] [Google Scholar]
  15. Karr R. W., Kannapell C. C., Stein J. A., Gebel H. M., Mann D. L., Duquesnoy R. J., Fuller T. C., Rodey G. E., Schwartz B. D. Molecular relationships of the human B cell alloantigens, MT2, MB3, MT4, and DR5. J Immunol. 1982 Apr;128(4):1809–1818. [PubMed] [Google Scholar]
  16. Klein J., Nagy Z. Physiology of alloreactivity: an interpretation. Transplant Proc. 1981 Mar;13(1 Pt 2):918–923. [PubMed] [Google Scholar]
  17. Kornbluth J., Silver D. M., Dupont B. Cloning and characterization of primary alloreactive human T-lymphocytes. Immunol Rev. 1981;54:111–155. doi: 10.1111/j.1600-065x.1981.tb00436.x. [DOI] [PubMed] [Google Scholar]
  18. Lamb J. R., Eckels D. D., Phelan M., Lake P., Woody J. N. Antigen-specific human T lymphocyte clones: viral antigen specificity of influenza virus-immune clones. J Immunol. 1982 Mar;128(3):1428–1432. [PubMed] [Google Scholar]
  19. Lublin D. M., Grumet F. C. Mechanisms of the CYNAP phenomenon: evidence in the Bw49/Bw50 model for epitopes with different spatial orientation of antibody. Hum Immunol. 1982 Apr;4(2):137–145. doi: 10.1016/0198-8859(82)90013-1. [DOI] [PubMed] [Google Scholar]
  20. Malissen B., Kristensen T., Goridis C., Madsen M., Mawas C. Clones of human cytotoxic T lymphocytes derived from an allosensitized individual: HLA specificity and cell surface markers. Scand J Immunol. 1981 Sep;14(3):213–224. doi: 10.1111/j.1365-3083.1981.tb00558.x. [DOI] [PubMed] [Google Scholar]
  21. Mann D. L., Kaufman J., Orr H., Robb R., Strominger J. Serologic and structural studies of DR-related antigens. Transplant Proc. 1979 Mar;11(1):668–673. [PubMed] [Google Scholar]
  22. Markert M. L., Cresswell P. Polymorphism of human B-cell alloantigens: evidence for three loci within the HLA system. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6101–6104. doi: 10.1073/pnas.77.10.6101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. McMichael A. J., Ting A., Zweerink H. J., Askonas B. A. HLA restriction of cell-mediated lysis of influenza virus-infected human cells. Nature. 1977 Dec 8;270(5637):524–526. doi: 10.1038/270524a0. [DOI] [PubMed] [Google Scholar]
  24. Morling N., Jakobsen B. K., Platz P., Ryder L. P., Svejgaard A., Thomsen M. Correlation between HLA-D/DR associated primed lymphocyte typing (PLT) defined DP-antigens, HLA-D and HLA-DR antigens. Tissue Antigens. 1981;17(4):421–427. doi: 10.1111/j.1399-0039.1981.tb00723.x. [DOI] [PubMed] [Google Scholar]
  25. Parham P., Barnstable C. J., Bodmer W. F. Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C, antigens. J Immunol. 1979 Jul;123(1):342–349. [PubMed] [Google Scholar]
  26. Parham P., McLean J. Characterization, evolution, and molecular basis of a polymorphic antigenic determinant shared by HLA-A and B products. Hum Immunol. 1980 Sep;1(2):131–139. doi: 10.1016/0198-8859(80)90100-7. [DOI] [PubMed] [Google Scholar]
  27. Pawelec G., Wernet P. Restimulation properties of human alloreactive cloned T-cell lines. Dissection of HLA-D-region alleles in population studies and in family segregation analysis. Immunogenetics. 1980;11(5):507–519. doi: 10.1007/BF01567819. [DOI] [PubMed] [Google Scholar]
  28. Quaranta V., Pellegrino M. A., Ferrone S. Serologic and immunochemical characterization of the specificity of four monoclonal antibodies to distinct antigenic determinants expressed on subpopulations of human Ia-like antigens. J Immunol. 1981 Feb;126(2):548–552. [PubMed] [Google Scholar]
  29. Quaranta V., Tanigaki N., Ferrone S. Distribution of antigenic determinants recognized by three monoclonal antibodies (Q2/70, Q5/6 and Q5/13) on human Ia-like alloantigens and on their subunits. Immunogenetics. 1981;12(1-2):175–182. doi: 10.1007/BF01561660. [DOI] [PubMed] [Google Scholar]
  30. Sheehy M. J., Sondel P. M., Bach M. L., Wank R., Bach F. H. HL-A LD (lymphocyte defined) typing: a rapid assay with primed lymphocytes. Science. 1975 Jun 27;188(4195):1308–1310. doi: 10.1126/science.124948. [DOI] [PubMed] [Google Scholar]
  31. Tanigaki N., Tosi R. The genetic control of human Ia alloantigens: a three-loci model derived from the immunochemical analysis of 'supertypic' specificities. Immunol Rev. 1982;66:5–37. doi: 10.1111/j.1600-065x.1982.tb00432.x. [DOI] [PubMed] [Google Scholar]
  32. Termijtelen A., van Leeuwen A., van Rood J. J. HLA-linked lymphocyte activating determinants. Immunol Rev. 1982;66:79–101. doi: 10.1111/j.1600-065x.1982.tb00435.x. [DOI] [PubMed] [Google Scholar]
  33. Termijtelen A., van Rood J. J. Complexity of stimulation in MLC and the influence of matching for HLA-A and -B. Scand J Immunol. 1981 Nov;14(5):459–466. [PubMed] [Google Scholar]
  34. Wank R., Schendel D. J., Blanco M. E., Dupont B. Secondary MLC responses of primed lymphocytes after selective sensitization to non-HLA-D determinants. Scand J Immunol. 1979;9(6):499–505. doi: 10.1111/j.1365-3083.1979.tb03277.x. [DOI] [PubMed] [Google Scholar]
  35. Widmer M. B., Bach F. H. Antigen-driven helper cell-independent cloned cytolytic T lymphocytes. Nature. 1981 Dec 24;294(5843):750–752. doi: 10.1038/294750a0. [DOI] [PubMed] [Google Scholar]
  36. Yunis E. J., Amos D. B. Three closely linked genetic systems relevant to transplantation. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3031–3035. doi: 10.1073/pnas.68.12.3031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Zeevi A., Chiu K. M., Duquesnoy R. J. Functional differences between cells from MLR colonies grown in soft agar cultures. J Immunol. 1980 Sep;125(3):1130–1135. [PubMed] [Google Scholar]
  38. Zeevi A., Chiu K. M., Duquesnoy R. J. Heterogeneity of cytolytic and suppressor clones of alloactivated cells generated from soft agar colonies. Hum Immunol. 1982 Oct;5(2):107–122. doi: 10.1016/0198-8859(82)90056-8. [DOI] [PubMed] [Google Scholar]
  39. Zeevi A., Scheffel C., Annen K., Bass G., Marrari M., Duquesnoy R. J. Association of PLT specificity of alloreactive lymphocyte clones with HLA-DR, MB and MT determinants. Immunogenetics. 1982;16(3):209–218. doi: 10.1007/BF00343310. [DOI] [PubMed] [Google Scholar]

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