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. 1983 Mar;80(6):1660–1664. doi: 10.1073/pnas.80.6.1660

Multiple mutations in the T region of the Agrobacterium tumefaciens tumor-inducing plasmid.

L W Ream, M P Gordon, E W Nester
PMCID: PMC393662  PMID: 6300864

Abstract

Three genetic loci affecting tumor morphology lie within pTiA6NC T-DNA: tms, tmr, and tml. Using deletions and multiple transposon insertions, we constructed tumor-inducing (Ti) plasmids representing every possible double and triple mutant combination. tms tmr and tms tmr tml mutants did not incite tumors on most plants and produced a very weak response on a few other hosts but tms tml and tmr tml mutants were virulent. Thus, either tms+ or tmr+ alone can promote significant tumor growth but tml+ by itself is not sufficient. On hosts where tms mutants induce tumors accompanied by shoot proliferation, addition of a tml mutation reduces or eliminates shoot proliferation, suggesting that tml+ promotes shoot development. The small calli incited by tms tmr and tms tmr tml mutants contain agropine, an indication that these plant cells incorporate T-DNA in the absence of substantial tumor growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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