Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Apr;80(7):1955–1959. doi: 10.1073/pnas.80.7.1955

Novel B family sequence from an early chorion cDNA library of Bombyx mori.

R Lecanidou, T H Eickbush, G C Rodakis, F C Kafatos
PMCID: PMC393730  PMID: 6572954

Abstract

A cDNA library has been constructed from transcripts present during the early stages of choriogenesis in the silk-moth, Bombyx mori. Clones representing novel sequences were selected by screening the library with (i) homotypic cDNA, (ii) rDNA and typical chorion probes of the A and B multigene families and (iii) selected clones from the library, which were positive in step (i) but negative in step (ii); this third step identified clones that hybridized to distinct subsets of the library. The sequence of one such clone was determined and shown to represent a novel subfamily of the B chorion multigene family, exclusively expressed during the early choriogenic period.

Full text

PDF
1955

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bock S. C., Tiemeier D. C., Mester K., Wu M., Goldsmith M. R. Hybridization-selected translation of Bombyx mori high-cysteine chorion proteins in Xenopus laevis oocytes. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1032–1036. doi: 10.1073/pnas.79.4.1032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eickbush T. H., Kafatos F. C. A walk in the chorion locus of Bombyx mori. Cell. 1982 Jun;29(2):633–643. doi: 10.1016/0092-8674(82)90179-9. [DOI] [PubMed] [Google Scholar]
  4. Griffin-Shea R., Thireos G., Kafatos F. C. Organization of a cluster of four chorion genes in Drosophila and its relationship to developmental expression and amplification. Dev Biol. 1982 Jun;91(2):325–336. doi: 10.1016/0012-1606(82)90039-2. [DOI] [PubMed] [Google Scholar]
  5. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Iatrou K., Tsitilou S. G., Goldsmith M. R., Kafatos F. C. Molecular analysis of the GrB mutation in Bombyx mori through the use of chorion cDNA library. Cell. 1980 Jul;20(3):659–669. doi: 10.1016/0092-8674(80)90312-8. [DOI] [PubMed] [Google Scholar]
  7. Iatrou K., Tsitilou S. G., Kafatos F. C. Developmental classes and homologous families of chorion genes in Bombyx mori. J Mol Biol. 1982 May 25;157(3):417–434. doi: 10.1016/0022-2836(82)90469-7. [DOI] [PubMed] [Google Scholar]
  8. Jones C. W., Kafatos F. C. Accepted mutations in a gene family: evolutionary diversification of duplicated DNA. J Mol Evol. 1982;19(1):87–103. doi: 10.1007/BF02100227. [DOI] [PubMed] [Google Scholar]
  9. Jones C. W., Kafatos F. C. Structure, organization and evolution of developmentally regulated chorion genes in a silkmoth. Cell. 1980 Dec;22(3):855–867. doi: 10.1016/0092-8674(80)90562-0. [DOI] [PubMed] [Google Scholar]
  10. Jones C. W., Rosenthal N., Rodakis G. C., Kafatos F. C. Evolution of two major chorion multigene families as inferred from cloned cDNA and protein sequences. Cell. 1979 Dec;18(4):1317–1332. doi: 10.1016/0092-8674(79)90242-3. [DOI] [PubMed] [Google Scholar]
  11. Manning R. F., Samols D. R., Gage L. P. The genes for 18S, 5.8S and 28S ribosomal RNA of Bombyx mori are organized into tandem repeats of uniform length. Gene. 1978 Oct;4(2):153–166. doi: 10.1016/0378-1119(78)90027-6. [DOI] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nadel M. R., Kafatos F. C. Specific protein synthesis in cellular differentiation. IV. The chorion proteins of Bombyx mori and their program of synthesis. Dev Biol. 1980 Mar;75(1):26–40. doi: 10.1016/0012-1606(80)90141-4. [DOI] [PubMed] [Google Scholar]
  14. Otsuka A. Recovery of DNA fragments inserted by the "tailing" method: regeneration of PstI restriction sites. Gene. 1981 May;13(4):339–346. doi: 10.1016/0378-1119(81)90013-5. [DOI] [PubMed] [Google Scholar]
  15. Regier J. C., Kafatos F. C., Goodfliesh R., Hood L. Silkmoth chorion proteins: sequence analysis of the products of a multigene family. Proc Natl Acad Sci U S A. 1978 Jan;75(1):390–394. doi: 10.1073/pnas.75.1.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rodakis G. C., Kafatos F. C. Origin of evolutionary novelty in proteins: how a high-cysteine chorion protein has evolved. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3551–3555. doi: 10.1073/pnas.79.11.3551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rodakis G. C., Moschonas N. K., Kafatos F. C. Evolution of a multigene family of chorion proteins in silkmoths. Mol Cell Biol. 1982 May;2(5):554–563. doi: 10.1128/mcb.2.5.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sim G. K., Kafatos F. C., Jones C. W., Koehler M. D., Efstratiadis A., Maniatis T. Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families. Cell. 1979 Dec;18(4):1303–1316. doi: 10.1016/0092-8674(79)90241-1. [DOI] [PubMed] [Google Scholar]
  19. Tsitilou S. G., Regier J. C., Kafatos F. C. Selection and sequence analysis of a cDNA clone encoding a known chorion protein of the A family. Nucleic Acids Res. 1980 May 10;8(9):1987–1997. doi: 10.1093/nar/8.9.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES