Table 1.
Strains and plasmids.
| Relevant characteristics1 | References | |
|---|---|---|
| Strain | ||
| Xanthomonas citri ssp. citri | IBSBF 1594; formerly known as Xanthomonas axonopodis pv. citri strain 306 (Xac); ApR | da Silva et al. (2002), Schaad et al. (2005, 2006) |
| E. coli DH10B | Cloning strain | Invitrogen |
| E. coli BL21(DE3) | Protein expression strain (pET system) | Novagen |
| Xac amy::pAPU1 | pHF5Ca-parB integrated into the amy locus of Xac; ApR KmR | This work |
| Xac parB::pAPU1 | pHF5Ca-parB integrated in parB; ApR KmR | |
| Xac parB::pAPU2 | pCR-2.1-TOPO-parB124-769 integrated in parB; ApR KmR | |
| Xac parB::pAPU3 | pPM7g-parB integrated in parB; ApR KmR | |
| Plasmids | ||
| pPM2a and pPM7g | GFP expression vectors; xylR pxyl gfpmut1 bla neo | Martins et al. (2010) |
| pHF5Ca | TAP-tag expression vector; xylR pxyl tap1479 bla neo | This work |
| pAPU1 | pHF5Ca-parB: xylR pxyl parB-tap1479 bla neo | |
| pAPU2 | Derivative of pCR-2.1-TOPO (Invitrogen) carrying parB124-769; bla neo | |
| pAPU3 | pPM7g-parB: xylR pxyl parB-gfpmut1 bla neo | |
1
ApR and KmR, ampicillin and kanamycin resistance, respectively; bla and neo, genes for β-lactamase and Neomycin phosphotransferase, respectively.