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Deletion within vfr eliminated the effect of serum on lasI/lasR expression at late but not early phase. PAO1 and PAOΔvfr were grown in either LB or LB/S to either early phase (A and C) or late phase (B and D). Samples were collected at each phase and the level of lasI (A and C) and lasR (B and D) expression was determined by qRT-PCR analysis. Values represent the means of three independent experiments ± SEM; ***P < 0.001.
