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. 1996 May 14;93(10):4913–4918. doi: 10.1073/pnas.93.10.4913

DNA analysis and diagnostics on oligonucleotide microchips.

G Yershov 1, V Barsky 1, A Belgovskiy 1, E Kirillov 1, E Kreindlin 1, I Ivanov 1, S Parinov 1, D Guschin 1, A Drobishev 1, S Dubiley 1, A Mirzabekov 1
PMCID: PMC39379  PMID: 8643503

Abstract

We present a further development in the technology of sequencing by hybridization to oligonucleotide microchips (SHOM) and its application to diagnostics for genetic diseases. A robot has been constructed to manufacture sequencing "microchips." The microchip is an array of oligonucleotides immobilized into gel elements fixed on a glass plate. Hybridization of the microchip with fluorescently labeled DNA was monitored in real time simultaneously for all microchip elements with a two-wavelength fluorescent microscope equipped with a charge-coupled device camera. SHOM has been used to detect beta-thalassemia mutations in patients by hybridizing PCR-amplified DNA with the microchips. A contiguous stacking hybridization technique has been applied for the detection of mutations; it can simplify medical diagnostics and enhance its reliability. The use of multicolor monitoring of contiguous stacking hybridization is suggested for large-scale diagnostics and gene polymorphism studies. Other applications of the SHOM technology are discussed.

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Selected References

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