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. 1983 Apr;80(8):2231–2234. doi: 10.1073/pnas.80.8.2231

Long-wavelength-absorbing forms of bacteriochlorophyll a in solutions of Triton X-100

J Gottstein 1, H Scheer 1,*
PMCID: PMC393792  PMID: 16578768

Abstract

At least three forms of Triton X-100-solubilized bacteriochlorophyll a (BChl a) have been characterized by UV/visible/near-IR absorption and CD spectra. One, absorbing at 770 nm, is similar to a monomeric solution in methanol. The two others have strongly red-shifted absorption peaks (860 nm and 930, 835 nm) and intense and complex CD bands in this region, indicative of strong interaction of at least two and three molecules of BChl a, respectively.

Keywords: photosynthesis, circular dichroism, chlorophyll micelle, chlorophyll aggregation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballshmiter K., Katz J. J. Long wavelength forms of chlorophyll. Nature. 1968 Dec 21;220(5173):1231–1233. doi: 10.1038/2201231a0. [DOI] [PubMed] [Google Scholar]
  2. COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
  3. Clayton R. K., Clayton B. J. B850 pigment-protein complex of Rhodopseudomonas sphaeroides: Extinction coefficients, circular dichroism, and the reversible binding of bacteriochlorophyll. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5583–5587. doi: 10.1073/pnas.78.9.5583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evans T. A., Katz J. J. Evidence for 5- and 6-coordinated magnesium in bacterio-chlorophyll a from visible absorption spectroscopy. Biochim Biophys Acta. 1975 Sep 8;396(3):414–426. doi: 10.1016/0005-2728(75)90147-4. [DOI] [PubMed] [Google Scholar]
  5. Feher G., Hoff A. J., Isaacson R. A., Ackerson L. C. ENDOR experiments on chlorophyll and bacteriochlorophyll in vitro and in the photosynthetic unit. Ann N Y Acad Sci. 1975 Apr 15;244:239–259. doi: 10.1111/j.1749-6632.1975.tb41534.x. [DOI] [PubMed] [Google Scholar]
  6. Feick R., Drews G. Isolation and characterization of light harvesting bacteriochlorophyll.protein complexes from Rhodopseudomonas capsulata. Biochim Biophys Acta. 1978 Mar 13;501(3):499–513. doi: 10.1016/0005-2728(78)90117-2. [DOI] [PubMed] [Google Scholar]
  7. Fong F. K. Molecular basis for the photosynthetic primary process. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3692–3695. doi: 10.1073/pnas.71.9.3692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayashi H., Nozawa T., Hatano M., Morita S. Circular dichroism of bacteriochlorophyll a in light harvesting bacteriochlorophyll protein complexes from Chromatium vinosum. J Biochem. 1981 Jun;89(6):1853–1861. doi: 10.1093/oxfordjournals.jbchem.a133387. [DOI] [PubMed] [Google Scholar]
  9. JACOBS E. E., HOLT A. S., KROMHOUT R., RABINOWITCH E. Spectroscopic properties of crystals and monolayers of chlorophyll and related compounds. Arch Biochem Biophys. 1957 Dec;72(2):495–511. doi: 10.1016/0003-9861(57)90225-4. [DOI] [PubMed] [Google Scholar]
  10. JACOBS E. E., VATTER A. E., HOLT A. S. Crystalline chlorophyll and bacteriochlorophyll. Arch Biochem Biophys. 1954 Nov;53(1):228–238. doi: 10.1016/0003-9861(54)90248-9. [DOI] [PubMed] [Google Scholar]
  11. KOMEN J. G. Observations on the infrared absorption spectrum of bacteriochlorophyll. Biochim Biophys Acta. 1956 Oct;22(1):9–15. doi: 10.1016/0006-3002(56)90216-5. [DOI] [PubMed] [Google Scholar]
  12. KRASNOVSKII A. A., VOINOVSKAIA K. K., KOSOBUTSKAIA L. M. Priroda ostestvennogo sostoianiia bakteriokhlorofilla s sviazi s spektral'nymi svoistvami ego kolloidnykh rastvorov i tverdykh planok. Dokl Akad Nauk SSSR. 1952 Jul 11;85(2):389–392. [PubMed] [Google Scholar]
  13. Katz J. J., Norris J. R., Shipman L. L., Thurnauer M. C., Wasielewski M. R. Chlorophyll function in the photosynthetic reaction center. Annu Rev Biophys Bioeng. 1978;7:393–434. doi: 10.1146/annurev.bb.07.060178.002141. [DOI] [PubMed] [Google Scholar]
  14. Massini P., Voorn G. Optical and photochemical properties of chlorophyll a solubilized in aqueous solutions of surfactants. Biochim Biophys Acta. 1968 Apr 2;153(3):589–601. doi: 10.1016/0005-2728(68)90187-4. [DOI] [PubMed] [Google Scholar]
  15. Norris J. R., Scheer H., Katz J. J. Models for antenna and reaction center chlorophylls. Ann N Y Acad Sci. 1975 Apr 15;244:260–280. doi: 10.1111/j.1749-6632.1975.tb41535.x. [DOI] [PubMed] [Google Scholar]
  16. Pearlstein R. M., Davis R. C., Ditson S. L. Giant circular dichroism of high molecular weight chlorophyllide-apomyoglobin complexes. Proc Natl Acad Sci U S A. 1982 Jan;79(2):400–402. doi: 10.1073/pnas.79.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reinach P., Aubrey B. B., Brody S. S. Monomolecular films of bacteriochlorophyll and derivatives at an air-water interface: surface and spectral properties. Biochim Biophys Acta. 1973 Sep 26;314(3):360–371. doi: 10.1016/0005-2728(73)90120-5. [DOI] [PubMed] [Google Scholar]
  18. Sato N., Murata N. Preparation of chlorophyll a, chlorophyll b and bacteriochlorophyll a by means of column chromatography with diethylaminoethylcellulose. Biochim Biophys Acta. 1978 Jan 11;501(1):103–111. doi: 10.1016/0005-2728(78)90099-3. [DOI] [PubMed] [Google Scholar]
  19. Terpstra W. Interaction of a spinach protein factor with bacteriochlorophyll. Biochim Biophys Acta. 1968 Apr 2;153(3):675–684. doi: 10.1016/0005-2728(68)90194-1. [DOI] [PubMed] [Google Scholar]
  20. Wasielewski M. R., Norris J. R., Shipman L. L., Lin C. P., Svec W. A. Monomeric chlorophyll a enol: Evidence for its possible role as the primary electron donor in photosystem I of plant photosynthesis. Proc Natl Acad Sci U S A. 1981 May;78(5):2957–2961. doi: 10.1073/pnas.78.5.2957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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