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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 May;80(9):2437–2441. doi: 10.1073/pnas.80.9.2437

Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase.

P Walter, J Gangloff, J Bonnet, Y Boulanger, J P Ebel, F Fasiolo
PMCID: PMC393840  PMID: 6341994

Abstract

The sequence of a 5-kilobase DNA insert containing the structural gene for yeast cytoplasmic methionyl-tRNA synthetase has been determined and a unique open reading frame of 2,253 nucleotides encoding a polypeptide chain of 751 amino acids (Mr, 85,500) has been characterized. The data obtained on the purified enzyme (subunit size, amino acid composition, and COOH-terminal sequence) are consistent with the gene structure. The protein sequence deduced from the nucleotide sequence reveals no obvious internal repeats. This protein sequence shows a high degree of homology with that of Escherichia coli methionyl-tRNA synthetase within a region that forms the putative methionyl adenylate binding site. This strongly suggests that both proteins derive from a common ancestor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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