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(A, B) Transient transfection assays were performed using promoter-luciferase constructs containing a serial of 5'-deletions (A) or mutations (B). (C) EMSA assays were performed using in vitro transcribed/translated proteins. Wild-type (WT) and mutant (MUT) oligos were used in the competition assays. (D) Chromatin immunoprecipitation (ChIP) assays were performed using liver lysates (n=3 per group). Akr1b7 serves as a positive control.
