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. 1983 May;80(9):2676–2680. doi: 10.1073/pnas.80.9.2676

Mutations in lambda repressor's amino-terminal domain: implications for protein stability and DNA binding.

M H Hecht, H C Nelson, R T Sauer
PMCID: PMC393890  PMID: 6221342

Abstract

The DNA binding properties of 52 different single-amino acid substitutions in lambda repressor's amino-terminal domain have been characterized. Seven proteins bearing mutations that change solvent-exposed side chains have been purified. The amino-terminal domains of these mutant repressors are folded and are comparable to the wild-type amino-terminal domain in thermal stability. In contrast, a purified mutant repressor bearing a substitution in a buried side chain contains an amino-terminal domain with decreased thermal stability. We argue that mutations that alter solvent-exposed wild-type side chains define residues that form the operator DNA binding surface of lambda repressor whereas completely or partially buried mutations exert their effect by decreasing protein stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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