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. 1983 May;80(10):2899–2901. doi: 10.1073/pnas.80.10.2899

Enzymic synthesis and cofactor activity of 3'-pyrophosphocoenzyme A.

J I Mukai, J Sy, F Lipmann
PMCID: PMC393940  PMID: 6574458

Abstract

The 3'-pyrophosphate derivative of CoA was synthesized by using the excreted 5'-to-3' pyrophosphoryl-transferring enzyme from Streptomyces adephospholyticus and ATP as donor and dephospho-CoA as acceptor. Cofactor activity of this new coenzyme A derivative was tested with Clostridium kluyveri phosphotransacetylase and hog heart succinic thiokinase. With the phosphotransacetylase, 3'-pyrophospho-CoA was found to be twice as active as CoA whereas dephospho-CoA was inactive. However, succinic thiokinase utilized all three types of CoA equally well. Adenosine 5'-monophosphate 3'-pyrophosphate also was synthesized and used as an analog of adenosine 5'-monophosphate 3'-monophosphate in the dog liver's sulfotransferase-catalyzed sulfate transfer from p-nitrophenyl sulfate to phenol. In contrast to the pyrophospho derivative of coenzyme A, adenosine 5'-monophosphate 3'-pyrophosphate was inactive as a cofactor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHA S., PARKS R. E., Jr SUCCINIC THIOKINASE. I. PURIFICATION OF THE ENZYME FROM PIG HEART. J Biol Chem. 1964 Jun;239:1961–1967. [PubMed] [Google Scholar]
  2. Cha S., Cha C. J., Parks R. E., Jr Succinic thiokinase. IV. Improved method of purification, arsenolysis of guanosine triphosphate, succinate-dependent guanosine triphos- phatase activity, and some other properties of the enzyme. J Biol Chem. 1967 Jun 10;242(11):2577–2581. [PubMed] [Google Scholar]
  3. Fehr S., Godt F., Isono K., Richter D. A ribosome-independent stringent factor from Bacillus stearothermophilus and a low molecular weight substance inhibitory to its activity. FEBS Lett. 1979 Jan 1;97(1):91–95. doi: 10.1016/0014-5793(79)80059-9. [DOI] [PubMed] [Google Scholar]
  4. GREGORY J. D., LIPMANN F. The transfer of sulfate among phenolic compounds with 3',5'-diphosphoadenosine as coenzyme. J Biol Chem. 1957 Dec;229(2):1081–1090. [PubMed] [Google Scholar]
  5. Gallant J. A. Stringent control in E. coli. Annu Rev Genet. 1979;13:393–415. doi: 10.1146/annurev.ge.13.120179.002141. [DOI] [PubMed] [Google Scholar]
  6. Grindey G. B., Nichol C. A. Micro procedure for determination of pyrophosphate and orthophosphate. Anal Biochem. 1970 Jan;33(1):114–119. doi: 10.1016/0003-2697(70)90444-6. [DOI] [PubMed] [Google Scholar]
  7. Moyer R. H., Smith R. A. Succinic thiokinase: evidence of a bound 32P-3'-dephosphocoenzyme A. Biochem Biophys Res Commun. 1966 Mar 8;22(5):603–609. doi: 10.1016/0006-291x(66)90318-4. [DOI] [PubMed] [Google Scholar]
  8. Mukai J. I., Razzaque A., Hanada Y., Murao S., Nishino T. Synthesis of deoxynucleoside-5'-tri-3'-diphosphates by Streptomyces adephospholyticus ATP:nucleotide pyrophosphokinase. Anal Biochem. 1980 May 1;104(1):136–140. doi: 10.1016/0003-2697(80)90288-2. [DOI] [PubMed] [Google Scholar]
  9. Mukai J., Kukita T., Murao S., Nishino T. Acceptor specificity of ATP:nucleoside-5'-phosphate pyrophosphotransferase from Streptomyces adephospholyticus. Synthesis of the 3'-pyrophosphates of pyrimidine nucleotides, some oligoribonucleotides, 5'-diphosphonucleosidic coenzymes and mG-5'-ppp-5'-Am. J Biochem. 1978 Apr;83(4):1209–1212. doi: 10.1093/oxfordjournals.jbchem.a132014. [DOI] [PubMed] [Google Scholar]
  10. NOSE Y., LIPMANN F. Separation of steroid sulfokinases. J Biol Chem. 1958 Dec;233(6):1348–1351. [PubMed] [Google Scholar]
  11. Razzaque A., Hanada Y., Mukai J. I., Murao S., Nishino T. NAD- and FAD-3'-pyrophosphates--enzymic synthesis and inertness. FEBS Lett. 1978 Sep 15;93(2):335–338. doi: 10.1016/0014-5793(78)81134-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SCHLESINGER M. J., COON M. J. Hydrolysis of nucleoside diand triphosphates by crystalline preparations of yeast inorganic pyrophosphatase. Biochim Biophys Acta. 1960 Jun 17;41:30–36. doi: 10.1016/0006-3002(60)90365-6. [DOI] [PubMed] [Google Scholar]
  13. Sy J., Akers H. Purification and properties of guanosine 5', 3'-polyphosphate synthetase from Bacillus brevis. Biochemistry. 1976 Oct 5;15(20):4399–4403. doi: 10.1021/bi00665a008. [DOI] [PubMed] [Google Scholar]
  14. Sy J., Lipmann F. Identification of the synthesis of guanosine tetraphosphate (MS I) as insertion of a pyrophosphoryl group into the 3'-position in guanosine 5'-diphosphate. Proc Natl Acad Sci U S A. 1973 Feb;70(2):306–309. doi: 10.1073/pnas.70.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]

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