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. 1983 May;80(10):3010–3014. doi: 10.1073/pnas.80.10.3010

Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.

A Razzaque, H Mizusawa, M M Seidman
PMCID: PMC393963  PMID: 6304690

Abstract

A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative frequency of colonies with plasmids but without galactokinase activity was determined. This assay showed that 1% of the plasmids were defective after passage in the mammalian cells. Individual mutant plasmids were examined and found to contain deletions, duplications, point mutations, and insertions of cell DNA.

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Selected References

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