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. 1983 May;80(10):3050–3053. doi: 10.1073/pnas.80.10.3050

Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells.

B L Hempstead, C W Parker, A Kulczycki Jr
PMCID: PMC393971  PMID: 6190168

Abstract

Purified rat serosal mast cells were sensitized with mouse immunoglobulin E (IgE) anti-2,4-dinitrophenyl antibody, partially depleted of phosphate, labeled with [32P]orthophosphate, and stimulated with dinitrophenylated bovine serum albumin or control protein. After 15-120 seconds at 37 degrees C, the cells were extracted with nonionic detergent. IgE receptors were purified by repetitive affinity chromatography and were analyzed by NaDodSO4/polyacrylamide gel electrophoresis and radioautography. Antigenic stimulation of intact rat mast cells produced a rapid and marked increase in the phosphorylation of the surface-exposed alpha component of the IgE receptor. However, phosphorylation of the 33,000 Mr beta component of the IgE receptor was not altered significantly by antigen stimulation. This suggests that the selective increase in phosphorylation of the IgE receptor alpha component may be part of the physiologic mediator secretion process triggered by antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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