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. 1983 May;80(10):3091–3095. doi: 10.1073/pnas.80.10.3091

Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence.

G W Both, J S Mattick, A R Bellamy
PMCID: PMC393980  PMID: 6304692

Abstract

Cloned DNA copies of the double-stranded RNA genomic segments of simian 11 rotavirus have been used to determine the coding assignment for VP7, the type-specific antigen of this virus. Translation of hybrid-selected mRNAs in an in vitro system supplemented with canine pancreatic microsomes permitted VP7 to be assigned to segment 9 and the two nonstructural viral proteins NCVP4 and NCVP3, to segments 7 and 8, respectively. Hybridization of cloned DNA probes for segments 7-9 with the corresponding segments of human rotavirus Wa confirmed these assignments. The complete nucleotide sequence of gene 9 has been determined. The deduced amino acid sequence reveals VP7 to be 326 amino acids in length with two NH2-terminal hydrophobic regions and a single glycosylation site at residues 69-71.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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