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. 1983 Jun;80(11):3163–3167. doi: 10.1073/pnas.80.11.3163

Complete nucleotide sequence of the 26S rRNA gene of Physarum polycephalum: its significance in gene evolution.

T Otsuka, H Nomiyama, H Yoshida, T Kukita, S Kuhara, Y Sakaki
PMCID: PMC394000  PMID: 6304693

Abstract

The complete nucleotide sequences of the 5.8S and 26S rRNA genes of Physarum polycephalum and the transcribed spacer between them were determined. Comparison of the sequences with those of the Escherichia coli 23S rRNA and yeast 26S rRNA genes showed that there are 16 highly homologous regions in the sequences of Physarum and E. coli and that eukaryotes have some eukaryote-specific extra sequences. The sequence immediately following the 5.8S-like region of E. coli 23S rRNA was found to be highly homologous to the 5' terminus of Physarum 26S rRNA, indicating that the eukaryote-specific 5.8S rRNA gene is derived from the 5'-terminal region of the prokaryote large rRNA gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allet B., Rochaix J. D. Structure analysis at the ends of the intervening DNA sequences in the chloroplast 23S ribosomal genes of C. reinhardii. Cell. 1979 Sep;18(1):55–60. doi: 10.1016/0092-8674(79)90353-2. [DOI] [PubMed] [Google Scholar]
  2. Brosius J., Dull T. J., Noller H. F. Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jan;77(1):201–204. doi: 10.1073/pnas.77.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cox R. A., Kelly J. M. Mature 23 SrRNA of prokaryotes appears homologous with the precursor of 25--28 rRNA of eukaryotes: comments on the evolution of 23--28 rRNA. FEBS Lett. 1981 Jul 20;130(1):1–6. doi: 10.1016/0014-5793(81)80652-7. [DOI] [PubMed] [Google Scholar]
  4. Edwards K., Kössel H. The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA. Nucleic Acids Res. 1981 Jun 25;9(12):2853–2869. doi: 10.1093/nar/9.12.2853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eperon I. C., Anderson S., Nierlich D. P. Distinctive sequence of human mitochondrial ribosomal RNA genes. Nature. 1980 Jul 31;286(5772):460–467. doi: 10.1038/286460a0. [DOI] [PubMed] [Google Scholar]
  6. Financsek I., Mizumoto K., Muramatsu M. Nucleotide sequence of the transcription initiation region of a rat ribosomal RNA gene. Gene. 1982 May;18(2):115–122. doi: 10.1016/0378-1119(82)90109-3. [DOI] [PubMed] [Google Scholar]
  7. Georgiev O. I., Nikolaev N., Hadjiolov A. A., Skryabin K. G., Zakharyev V. M., Bayev A. A. The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisae. Nucleic Acids Res. 1981 Dec 21;9(24):6953–6958. doi: 10.1093/nar/9.24.6953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gourse R. L., Gerbi S. A. Fine structure of ribosomal RNA. III. Location of evolutionarily conserved regions within ribosomal DNA. J Mol Biol. 1980 Jun 25;140(2):321–339. doi: 10.1016/0022-2836(80)90109-6. [DOI] [PubMed] [Google Scholar]
  9. Hall L., Braun R. The organisation of genes for transfer RNA and ribosomal RNA in amoebae and plasmodia of Physarum polycephalum. Eur J Biochem. 1977 Jun 1;76(1):165–174. doi: 10.1111/j.1432-1033.1977.tb11582.x. [DOI] [PubMed] [Google Scholar]
  10. Jacq B. Sequence homologies between eukaryotic 5.8S rRNA and the 5' end of prokaryotic 23S rRNa: evidences for a common evolutionary origin. Nucleic Acids Res. 1981 Jun 25;9(12):2913–2932. doi: 10.1093/nar/9.12.2913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelly J. M., Cox R. A. The nucleotide sequence at the 3'-end of Neurospora crassa 25S-rRNA and the location of a 5.8S-rRNA binding site. Nucleic Acids Res. 1981 Mar 11;9(5):1111–1121. doi: 10.1093/nar/9.5.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Komiya H., Takemura S. The nucleotide sequence of 5S ribosomal RNA from slime mold Physarum polycephalum. J Biochem. 1981 Dec;90(6):1577–1581. doi: 10.1093/oxfordjournals.jbchem.a133631. [DOI] [PubMed] [Google Scholar]
  13. Kukita T., Sakaki Y., Nomiyama H., Otsuka T., Kuhara S., Takagi Y. Structure around the 3' terminus of the 26S ribosomal RNA gene of Physarum polycephalum. Gene. 1981 Dec;16(1-3):309–315. doi: 10.1016/0378-1119(81)90086-x. [DOI] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Nazar R. N. A 5.8 S rRNA-like sequence in prokaryotic 23 S rRNA. FEBS Lett. 1980 Oct 6;119(2):212–214. doi: 10.1016/0014-5793(80)80254-7. [DOI] [PubMed] [Google Scholar]
  16. Nomiyama H., Kuhara S., Kukita T., Otsuka T., Sakaki Y. Nucleotide sequence of the ribosomal RNA gene of Physarum polycephalum: intron 2 and its flanking regions of the 26S rRNA gene. Nucleic Acids Res. 1981 Nov 11;9(21):5507–5520. doi: 10.1093/nar/9.21.5507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nomiyama H., Sakaki Y., Takagi Y. Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1376–1380. doi: 10.1073/pnas.78.3.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Otsuka T., Nomiyama H., Sakaki Y., Takagi Y. Nucleotide sequence of Physarum polycephalum 5.8S rRNA gene and its flanking regions. Nucleic Acids Res. 1982 Apr 10;10(7):2379–2385. doi: 10.1093/nar/10.7.2379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sakaki Y., Nakamura M., Nomiyama H., Takagi Y. Cloning and characterization of ribosomal RNA gene of Physarum polycephalum. J Biochem. 1981 Jun;89(6):1693–1698. doi: 10.1093/oxfordjournals.jbchem.a133369. [DOI] [PubMed] [Google Scholar]
  20. Sollner-Webb B., Reeder R. H. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis. Cell. 1979 Oct;18(2):485–499. doi: 10.1016/0092-8674(79)90066-7. [DOI] [PubMed] [Google Scholar]
  21. Staden R. Sequence data handling by computer. Nucleic Acids Res. 1977 Nov;4(11):4037–4051. doi: 10.1093/nar/4.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Veldman G. M., Klootwijk J., de Regt V. C., Planta R. J., Branlant C., Krol A., Ebel J. P. The primary and secondary structure of yeast 26S rRNA. Nucleic Acids Res. 1981 Dec 21;9(24):6935–6952. doi: 10.1093/nar/9.24.6935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wild M. A., Sommer R. Sequence of a ribosomal RNA gene intron from Tetrahymena. Nature. 1980 Feb 14;283(5748):693–694. doi: 10.1038/283693a0. [DOI] [PubMed] [Google Scholar]

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