Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Jun;80(11):3470–3474. doi: 10.1073/pnas.80.11.3470

A monoclonal antibody-enzyme immunoassay for serum carcinoembryonic antigen with increased specificity for carcinomas.

A Hedin, L Carlsson, A Berglund, S Hammarström
PMCID: PMC394066  PMID: 6344085

Abstract

A two-site monoclonal antibody-enzyme immunoassay (MEIA) for carcinoembryonic antigen (CEA) was developed that uses two monoclonal anti-CEA antibodies, which recognize two different epitopes in the peptide moiety of CEA. The assay was sensitive to 0.5 micrograms/liter and had a measuring range of 0.5-200 micrograms of CEA per liter. It was highly specific inasmuch as none of three known CEA-related substances, "nonspecific crossreacting antigens 1 and 2" (NCA-1 and NCA-2) and biliary glycoprotein I (BGP I), reacted in the assay. NCA-2 (meconium antigen) is very similar to CEA. None of five commercially available CEA assays were able to differentiate between CEA and NCA-2. With one exception (colon), normal tissue extracts did not react in the MEIA even when tested at very high concentrations. Sera from a total of 180 healthy individuals and patients with malignant and nonmalignant disease were analyzed for CEA levels by using the MEIA and in parallel a conventional radioimmunoassay. A significant increase in specificity for carcinomas was obtained with the MEIA. This was essentially due to a decrease of MEIA CEA values in sera from patients with nonmalignant disease. The CEA values in the group of carcinoma patients (colon, pancreas, lung, and breast) were the same in the two assays.

Full text

PDF
3470

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Accolla R. S., Carrel S., Mach J. P. Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines. Proc Natl Acad Sci U S A. 1980 Jan;77(1):563–566. doi: 10.1073/pnas.77.1.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buchegger F., Phan M., Rivier D., Carrel S., Accolla R. S., Mach J. P. Monoclonal antibodies against carcinoembryonic antigen (CEA) used in a solid-phase enzyme immunoassay. First clinical results. J Immunol Methods. 1982 Mar 12;49(2):129–139. doi: 10.1016/0022-1759(82)90271-x. [DOI] [PubMed] [Google Scholar]
  3. Burtin P., Chavanel G., Hirsch-Marie H. Characterization of a second normal antigen that cross-reacts with CEA. J Immunol. 1973 Dec;111(6):1926–1928. [PubMed] [Google Scholar]
  4. Carlsson H. E., Lindberg A. A., Hammarström S. Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay. Infect Immun. 1972 Nov;6(5):703–708. doi: 10.1128/iai.6.5.703-708.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carlsson J., Drevin H., Axén R. Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. Biochem J. 1978 Sep 1;173(3):723–737. doi: 10.1042/bj1730723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Egan M. L., Pritchard D. G., Todd C. W., Go V. L. Isolation and immunochemical and chemical characterization of carcinoembryonic antigen-like substances in colon lavages of healthy individuals. Cancer Res. 1977 Aug;37(8 Pt 1):2638–2643. [PubMed] [Google Scholar]
  7. Fritsche R., Mach J. P. Isolation and characterization of carcinoembryonic antigen (CEA) extracted from normal human colon mucosa. Immunochemistry. 1977 Feb;14(2):119–127. doi: 10.1016/0019-2791(77)90290-7. [DOI] [PubMed] [Google Scholar]
  8. Hedin A., Hammarström S., Larsson A. Specificities and binding properties of eight monoclonal antibodies against carcinoembryonic antigen. Mol Immunol. 1982 Dec;19(12):1641–1648. doi: 10.1016/0161-5890(82)90275-9. [DOI] [PubMed] [Google Scholar]
  9. Kupchik H. Z., Zurawski V. R., Jr, Hurrell J. G., Zamcheck N., Black P. H. Monoclonal antibodies to carcinoembryonic antigen produced by somatic cell fusion. Cancer Res. 1981 Sep;41(9 Pt 1):3306–3310. [PubMed] [Google Scholar]
  10. Kuroki M., Koga Y., Matsuoka Y. Purification and characterization of carcinoembryonic antigen-related antigens in normal adult feces. Cancer Res. 1981 Feb;41(2):713–720. [PubMed] [Google Scholar]
  11. Lindgren J., Hång B., Hurme M., Mäkelä O. Monoclonal antibodies to carcinoembryonic antigen (CEA): characterization and use in a radioimmunoassay for CEA. Acta Pathol Microbiol Immunol Scand C. 1982 Jun;90(3):159–162. doi: 10.1111/j.1699-0463.1982.tb01433.x. [DOI] [PubMed] [Google Scholar]
  12. Mach J. P., Pusztaszeri G. Carcinoembryonic antigen (CEA): demonstration of a partial identity between CEA and a normal glycoprotein. Immunochemistry. 1972 Oct;9(10):1031–1034. doi: 10.1016/0019-2791(72)90113-9. [DOI] [PubMed] [Google Scholar]
  13. Matsuoka Y., Kuroki M., Koga Y., Kuriyama H., Mori T., Kosaki G. Immunochemical differences among carcinoembryonic antigen in tumor tissues and related antigens in meconium and adult feces. Cancer Res. 1982 May;42(5):2012–2018. [PubMed] [Google Scholar]
  14. Rogers G. T., Rawlins G. A., Bagshawe K. D. Somatic-cell hybrids producing antibodies against CEA. Br J Cancer. 1981 Jan;43(1):1–4. doi: 10.1038/bjc.1981.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rogers G. T., Rawlins G. A., Keep P. A., Cooper E. H., Bagshawe K. D. Application of monoclonal antibodies to purified CEA in clinical radioimmunoassay of human serum. Br J Cancer. 1981 Sep;44(3):371–380. doi: 10.1038/bjc.1981.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Svenberg T., Hammarström S., Hedin A. Purification and properties of biliary glycoprotein I (BGP I). Immunochemical relationship to carcinoembryonic antigen. Mol Immunol. 1979 Apr;16(4):245–252. doi: 10.1016/0161-5890(79)90063-4. [DOI] [PubMed] [Google Scholar]
  17. von Kleist S., Chavanel G., Burtin P. Identification of an antigen from normal human tissue that crossreacts with the carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2492–2494. doi: 10.1073/pnas.69.9.2492. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES