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. 2014 Feb 3;3(2):e85. doi: 10.1038/oncsis.2013.49

Figure 5.

Figure 5

Phosphomimetic Chk2 alleviates dysregulation of microtubule nucleation in DNA-PKcs−/− cells. (a) HCT116 and DNA-PKcs−/− cells were treated with nocodazole to disrupt microtubules. Microtubule nucleation and regrowth was monitored at the indicated time points after nocodazole removal. (b) The length of the microtubule emanating from the centrosomes was measured (n⩾50). (c) HeLa cells expressing wild-type Chk2 or T68A or T68D mutant Chk2 were transfected with control or DNA-PKcs-targeted siRNA. Forty-eight hours after transfection, cells were subjected to microtubule nucleation analysis (n⩾50). *P<0.05; **P<0.01.