Figure 4. Morpholino knockdown of Nv eve, Nv hairy, and Nv odd results in embryo patterning defects.
First instar larval cuticles are shown with anterior left and generally ventral denticle patterns are shown. (A, F, K) Wild-type larval cuticles. Yellow arrows indicate spiracles present on segments T2, A1, A2 and A3. Bright anterior labral appendages are apparent at the extreme anterior of the larva. (B–E) Unhatched larvae from Nv eve morpholino (MO)-injected embryos, in order of increasing phenotype severity. Red arrowheads indicate loss of midline cuticle. Blue dot indicates head open defect. Yellow arrowheads indicate position of spiracles. (G–J) Unhatched larvae from Nv odd morpholino (MO)-injected embryos, in order of increasing phenotype severity. Yellow arrows indicate position of spiracles, red arrows indicate A3/A4 fusion. X indicates naked cuticle from segment loss. Yellow line indicates multi-segment fusion. (L–O) Unhatched larvae from Nv hairy morpholino (MO)-injected embryos, in order of increasing phenotype severity. Yellow arrowheads indicate position of spiracles. Red or orange arrowheads indicate aberrantly positioned or missing spiracles. Yellow line indicates segment fusion.