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. 1983 Jun;80(12):3782–3786. doi: 10.1073/pnas.80.12.3782

Macrophage activation: priming activity from a T-cell hybridoma is attributable to interferon-gamma.

J L Pace, S W Russell, R D Schreiber, A Altman, D H Katz
PMCID: PMC394136  PMID: 6407020

Abstract

Antiviral and macrophage-priming activities in the supernatant medium of a subclone of a concanavalin A-stimulated mouse T-cell hybridoma were investigated. The two activities were associated with a molecular weight of approximately 50,000 and could not be separated by various approaches. Both activities were eliminated by a highly specific neutralizing antibody against mouse interferon-gamma, but not by antibody against interferon-alpha and -beta. The ratio of priming to antiviral activity in the hybridoma culture supernate was indistinguishable from the ratio obtained with mouse interferon-gamma prepared by recombinant DNA technology. It was concluded from these data that the priming activity in hybridoma culture supernates was attributable to interferon-gamma and that this mediator is one form of the lymphokine macrophage-activating factor. Interferon-gamma was greater than 800 times more efficient at priming mouse macrophages for tumor cell killing than was a mixture of interferon-alpha and -beta. This finding contributes to growing awareness that type II interferon may have greater immunoregulatory potential than type I interferons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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