Abstract
Quantitative autoradiography shows that there is a close relationship between [3H]imipramine binding sites and the distribution of 5-hydroxytryptamine (5-HT) neurons in the rat brain. High labeling is observed in the midbrain raphe nuclei, the areas of the dopamine cell groups of the substantia nigra and of the ventral tegmental area of Tsai, the ventral amygdaloid nucleus, the midline thalamic area, and parts of the hypothalmus. Thus, antidepressant drugs that have high affinity for [3H]imipramine binding sites can exert an influence at the 5-HT cell body as well as at the 5-HT nerve terminal level. The present results underline the possibility that the 5-HT and dopamine hypotheses for the mechanism of action of antidepressant drugs are not mutually exclusive, because both 5-HT and dopamine neurons can be regulated by large numbers of [3H]imipramine binding sites.
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- Alexander G. M., Schwartzman R. J., Bell R. D., Yu J., Renthal A. Quantitative measurement of local cerebral metabolic rate for glucose utilizing tritiated 2-deoxyglucose. Brain Res. 1981 Oct 26;223(1):59–67. doi: 10.1016/0006-8993(81)90806-4. [DOI] [PubMed] [Google Scholar]
- Fuxe K., Hökfelt T., Olson L., Ungerstedt U. Central monoaminergic pathways with emphasis on their relation to the so called 'extrapyramidal motor system'. Pharmacol Ther B. 1977;3(2):169–210. doi: 10.1016/0306-039x(77)90032-0. [DOI] [PubMed] [Google Scholar]
- Fuxe K., Ungerstedt U. Histochemical studies on the distribution of catecholamines and 5-hydroxytryptamine after intraventricular injections. Histochemie. 1968;13(1):16–28. doi: 10.1007/BF00303872. [DOI] [PubMed] [Google Scholar]
- Ogren S. O., Fuxe K., Agnati L. F., Gustafsson J. A., Jonsson G., Holm A. C. Reevaluation of the indoleamine hypothesis of depression. Evidence for a reduction of functional activity of central 5-HT systems by antidepressant drugs. J Neural Transm. 1979;46(2):85–103. doi: 10.1007/BF01250331. [DOI] [PubMed] [Google Scholar]
- Raisman R., Briley M., Langer S. Z. High-affinity 3H-imipramine binding in rat cerebral cortex. Eur J Pharmacol. 1979 Mar 1;54(3):307–308. doi: 10.1016/0014-2999(79)90092-x. [DOI] [PubMed] [Google Scholar]
- Steinbusch H. W. Distribution of serotonin-immunoreactivity in the central nervous system of the rat-cell bodies and terminals. Neuroscience. 1981;6(4):557–618. doi: 10.1016/0306-4522(81)90146-9. [DOI] [PubMed] [Google Scholar]
- Unnerstall J. R., Niehoff D. L., Kuhar M. J., Palacios J. M. Quantitative receptor autoradiography using [3H]ultrofilm: application to multiple benzodiazepine receptors. J Neurosci Methods. 1982 Jul;6(1-2):59–73. doi: 10.1016/0165-0270(82)90016-4. [DOI] [PubMed] [Google Scholar]