Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Jul;80(13):3953–3957. doi: 10.1073/pnas.80.13.3953

Partial mRNA sequences for human A alpha, B beta, and gamma fibrinogen chains: evolutionary and functional implications.

J A Kant, S T Lord, G R Crabtree
PMCID: PMC394177  PMID: 6575389

Abstract

Using rat cDNA and genomic probes to screen a human liver cDNA library, we have isolated clone of 2,274, 855, and 736 base pairs (bp) coding for the A alpha, B beta and gamma chains of human fibrinogen. Sequence analysis reveals a hitherto unrecognized extension of 15 amino acids at the carboxyl terminus of the A alpha chain, the terminal residue of which is proline. This brings the known length of the human A alpha chain to 625 amino acids. The 13-amino-acid repeated region in the midportion of the A alpha chain clearly has arisen through an 8-fold duplication of a 39-bp genetic element, which itself appears to have been constructed from smaller 6-bp repeating units. Greater than 50% sequence homology between B beta- and gamma-chain coding regions confirms postulates that these genes have arisen by duplication and subsequent divergence of an ancestral gene. A comparison of human and rat gamma-chain cDNAs shows more than 88% sequence homology over the carboxyl-terminal 162 amino acids, implying strong selective pressures on these portions of the gamma-chain gene.

Full text

PDF
3953

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  2. Blombäck B., Hogg D. H., Gårdlund B., Hessel B., Kudryk B. Fibrinogen and fibrin formation. Thromb Res. 1976 May;8(2 Suppl):329–346. doi: 10.1016/0049-3848(76)90075-x. [DOI] [PubMed] [Google Scholar]
  3. Crabtree G. R., Kant J. A. Molecular cloning of cDNA for the alpha, beta, and gamma chains of rat fibrinogen. A family of coordinately regulated genes. J Biol Chem. 1981 Sep 25;256(18):9718–9723. [PubMed] [Google Scholar]
  4. Crabtree G. R., Kant J. A. Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen. Cell. 1982 Nov;31(1):159–166. doi: 10.1016/0092-8674(82)90415-9. [DOI] [PubMed] [Google Scholar]
  5. Davidson J. M., McEneany L. S., Bornstein P. Intermediates in the limited proteolytic conversion of procollagen to collagen. Biochemistry. 1975 Nov 18;14(23):5188–5194. doi: 10.1021/bi00694a026. [DOI] [PubMed] [Google Scholar]
  6. Doolittle R. F. Similar amino acid sequences: chance or common ancestry? Science. 1981 Oct 9;214(4517):149–159. doi: 10.1126/science.7280687. [DOI] [PubMed] [Google Scholar]
  7. Doolittle R. F., Watt K. W., Cottrell B. A., Strong D. D., Riley M. The amino acid sequence of the alpha-chain of human fibrinogen. Nature. 1979 Aug 9;280(5722):464–468. doi: 10.1038/280464a0. [DOI] [PubMed] [Google Scholar]
  8. Eiferman F. A., Young P. R., Scott R. W., Tilghman S. M. Intragenic amplification and divergence in the mouse alpha-fetoprotein gene. Nature. 1981 Dec 24;294(5843):713–718. doi: 10.1038/294713a0. [DOI] [PubMed] [Google Scholar]
  9. Galanakis D. K., Mosesson M. W., Stathakis N. E. Human fibrinogen heterogeneities: distribution and charge characteristics of chains of A alpha origin. J Lab Clin Med. 1978 Sep;92(3):376–386. [PubMed] [Google Scholar]
  10. Henschen A., Lottspeich F., Hessel B. Amino acid sequence of human fibrin. Preliminary note on the completion of the intermediate part of the alpha-chain sequence. Hoppe Seylers Z Physiol Chem. 1979 Dec;360(12):1951–1956. [PubMed] [Google Scholar]
  11. Henschen A., Lottspeich F., Töpfer-Petersen E., Warbinek R. Primary structure of fibrinogen. Thromb Haemost. 1979 Jun 30;41(4):662–670. [PubMed] [Google Scholar]
  12. Kant J. A., Crabtree G. R. The rat fibrinogen genes. Linkage of the A alpha and gamma chain genes. J Biol Chem. 1983 Apr 25;258(8):4666–4667. [PubMed] [Google Scholar]
  13. Kloczewiak M., Timmons S., Hawiger J. Localization of a site interacting with human platelet receptor on carboxy-terminal segment of human fibrinogen gamma chain. Biochem Biophys Res Commun. 1982 Jul 16;107(1):181–187. doi: 10.1016/0006-291x(82)91686-2. [DOI] [PubMed] [Google Scholar]
  14. Lottspeich F., Henschen A. Amino acid sequence of human fibrin. Preliminary note on an internal peptide obtained by cleaving the gamma-chain at the arginyl bonds and showing sequence homology with the C-terminus. Hoppe Seylers Z Physiol Chem. 1977 Jun;358(6):703–707. [PubMed] [Google Scholar]
  15. Maizel J. V., Jr, Lenk R. P. Enhanced graphic matrix analysis of nucleic acid and protein sequences. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7665–7669. doi: 10.1073/pnas.78.12.7665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marguerie G. A., Ardaillou N., Cherel G., Plow E. F. The binding of fibrinogen to its platelet receptor. J Biol Chem. 1982 Oct 25;257(20):11872–11875. [PubMed] [Google Scholar]
  17. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  19. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mosesson M. W., Galanakis D. K., Finlayson J. S. Comparison of human plasma fibrinogen subfractions and early plasmic fibrinogen derivatives. J Biol Chem. 1974 Jul 25;249(14):4656–4664. [PubMed] [Google Scholar]
  21. Ohno S. Original domain for the serum albumin family arose from repeated sequences. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7657–7661. doi: 10.1073/pnas.78.12.7657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pustell J., Kafatos F. C. A high speed, high capacity homology matrix: zooming through SV40 and polyoma. Nucleic Acids Res. 1982 Aug 11;10(15):4765–4782. doi: 10.1093/nar/10.15.4765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Queen C. L., Korn L. J. Computer analysis of nucleic acids and proteins. Methods Enzymol. 1980;65(1):595–609. doi: 10.1016/s0076-6879(80)65062-9. [DOI] [PubMed] [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thayer R. E. An improved method for detecting foreign DNA in plasmids of Escherichia coli. Anal Biochem. 1979 Sep 15;98(1):60–63. doi: 10.1016/0003-2697(79)90705-x. [DOI] [PubMed] [Google Scholar]
  26. Wozney J., Hanahan D., Morimoto R., Boedtker H., Doty P. Fine structural analysis of the chicken pro alpha 2 collagen gene. Proc Natl Acad Sci U S A. 1981 Feb;78(2):712–716. doi: 10.1073/pnas.78.2.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yamada Y., Avvedimento V. E., Mudryj M., Ohkubo H., Vogeli G., Irani M., Pastan I., de Crombrugghe B. The collagen gene: evidence for its evolutinary assembly by amplification of a DNA segment containing an exon of 54 bp. Cell. 1980 Dec;22(3):887–892. doi: 10.1016/0092-8674(80)90565-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES