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. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

M D Biggin, T J Gibson, G F Hong
PMCID: PMC394179  PMID: 6575390

Abstract

Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. We also show that the use of deoxyadenosine 5'-(alpha-[35S]thio)triphosphate as the label incorporated in dideoxynucleotide sequence reactions increases the sharpness of the bands on an autoradiograph and so increases the resolution achieved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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