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Overexpression of RhoGDIα downregulates prostate-specific antigen (PSA) protein expression. C4-2, C4-2 neo, and C4-2 GDI-C2 cells overexpressing RhoGDIα were cultured in FBS or charcoal-stripped FBS in the presence or absence of 1 nM DHT. Supernatants were collected and subjected to enzyme-linked immunosorbent assay (ELISA) for PSA proteins. Bottom panel shows immunoblot of whole cell extracts with RhoGDIα and tubulin antibodies.
