Genetic and phenotypic variations of inherited retinal diseases in dogs: the power of within- and across-breed studies

Abstract

Considerable clinical and molecular variations have been known in retinal blinding diseases in man and also in dogs. Different forms of retinal diseases occur in specific breed(s) caused by mutations segregating within each isolated breeding population. While molecular studies to find genes and mutations underlying retinal diseases in dogs have benefited largely from the phenotypic and genetic uniformity within a breed, within- and across-breed variations have often played a key role in elucidating the molecular basis. The increasing knowledge of phenotypic, allelic, and genetic heterogeneities in canine retinal degeneration has shown that the overall picture is rather more complicated than initially thought. Over the past 20 years, various approaches have been developed and tested to search for genes and mutations underlying genetic traits in dogs, depending on the availability of genetic tools and sample resources. Candidate gene, linkage analysis, and genome-wide association studies have so far identified 24 mutations in 18 genes underlying retinal diseases in at least 58 dog breeds. Many of these genes have been associated with retinal diseases in humans, thus providing opportunities to study the role in pathogenesis and in normal vision. Application in therapeutic interventions such as gene therapy has proven successful initially in a naturally occurring dog model followed by trials in human patients. Other genes whose human homologs have not been associated with retinal diseases are potential candidates to explain equivalent human diseases and contribute to the understanding of their function in vision.

Introduction

The past 20 years have seen a virtual explosion in gene discovery of inherited retinal diseases (RDs) in humans (Fig. 1). In man, these are clinically heterogeneous, characterized by defects in different retinal cell types, variable in age of onset, and diverse degrees of severity. These inherited diseases, subdivided by aspects of the clinical phenotype observed, include retinitis pigmentosa (RP), cone–rod dystrophy (CRD), and Leber congenital amaurosis (LCA), among others.

Fig. 1.

Accumulation of the numbers of mapped loci (light gray) and identified genes (dark gray) in human RDs from 1980 to 2011. Reprinted from RetNet, http://www.sph.uth.tmc.edu/RetNet, with permission (Stephen P. Daiger, PhD, and the University of Texas Health Science Center at Houston)

Since the mapping of an autosomal dominant RP locus to the RHO (rhodopsin) gene (Farrar et al. 1990; McWilliam et al. 1989), at least 213 retinal disease loci/172 genes have been independently associated with human RDs (RetNet, http://www.sph.uth.tmc.edu/RetNet/) (Supplementary Fig. 1). In some of these genes, over 100 mutations have been identified. Such extensive genetic and allelic heterogeneities likely reflect the observed clinical heterogeneity.

Dogs also suffer from various forms of naturally occurring RDs causing blindness, with the most common known disease class in the veterinary field being progressive retinal atrophy (PRA). Over 100 breeds are affected with PRA, and all affected dogs show clinically recognizable abnormalities and behavioral signs indicative of visual deficits at various ages and with different degrees of progression. Since PRA was identified as a clinical entity by Magnusson in the early 20th century, it was recognized as the homolog to RP in humans, and the diseases in both species shared genetic, clinical, and pathological features (Magnusson 1909, 1910, 1911, 1917). Remarkable early progress in establishing the dog as a model for RD research was accomplished by Parry in a series of seminal studies published in the mid-20th century (Parry 1953a, b, 1954a, b; Parry et al. 1953, 1955), and the history and the evolution of research in this field has been summarized more recently (Aguirre and Acland 2006).

Pedigree dog breeds have unique breeding histories over the past 100–300 years, with strong effects from popular sires as well as breed barriers. As a result, most dogs of the same breed are relatively uniform genetically, just like certain isolated human populations in which many sequence variants are shared across the population. This within-breed genetic uniformity has been advantageous in studies of canine genetic traits, including RDs. While human RDs encompass a broad phenotypic and genetic spectrum making it difficult to isolate and dissect each form, the diseases within a dog breed often are phenotypically and genetically uniform and thus more tractable. With canine RDs considered homologs to those of man, molecular studies in dogs have continued to contribute to the understanding of the human diseases.

The within-breed phenotypic and genetic uniformities have contributed much to genetic studies of canine RDs and, at the same time, served to highlight heterogenic characteristics suggestive of additional diseases in a breed. For example, genetic heterogeneity is seen in Golden retrievers which were thought to be affected with progressive rod–cone degeneration (prcd) only, but at least two other forms of PRA, including GR_PRA1, are recognized now in the breed (Downs et al. 2011). However, the phenotypic and genetic heterogeneities within the same RD form, as well as within a breed, are not as extensive or complex as in human populations, making it possible to carry out gene discovery studies once the phenotypes and genetics are properly ascertained.

In this article we review the molecular participants involved in the complex retinal disease system, classify the clinically distinct forms of canine RDs, and trace the different approaches developed and applied for disease gene discovery. We dissect the heterogenic nature that has become increasingly evident in canine RDs, showing examples of its effective application to gene discovery.

Structural and functional elements of the retina: a key to understanding diseases and pathways

The retina

Dogs are born with an undeveloped retina that reaches maturity at 3–6 weeks postnatally (Aguirre et al. 1972; Parry 1953a), whereas humans are born with a retina that is much further developed and undergoes further postnatal differentiation, particularly of the fovea and macula (Abramov et al. 1982). Nevertheless, the structure of the mature canine retina is broadly similar to that of man, consisting of layers of specialized cells (Fig. 2a, b). The outermost layer, comprising the light-sensitive rod and cone photoreceptors, abuts and is nourished by the retinal pigment epithelium (RPE). Each photoreceptor has outer (OS) and inner (IS) segments. Phototransduction is initiated on the stacked disks of the OS while the biosynthetic functions are concentrated in the IS. The connecting cilium, bridging the OS and the IS, has an important role in intraflagellar transport (see Webvision for further details: http://webvision.med.utah.edu/). Of the photoreceptor cells, cones are responsible for day and color vision, whereas rods confer night or dim light vision. In both humans and dogs, rods and cones are unevenly distributed across the retina and the number of rods by far exceeds the number of cones (Curcio et al. 1990; Mowat et al. 2008).

Fig. 2.

The structural and functional elements of the retina and the localization of selected genes involved in canine retinal degeneration. a Histologic section of the canine retina showing the highly ordered lamination from the outer (top) to the inner (bottom) retina. The rod and cone photoreceptors that form the outermost layer receive the light stimuli and are nourished by the retinal pigment epithelium (RPE). The outer nuclear layer (ONL) contains the nuclei and cell bodies of the photoreceptors. The inner nuclear layer (INL) contains the nuclei of the secondary neurons (i.e., bipolar, amacrine, and horizontal cells) and the glial Müller cells. The innermost ganglion cell layer (GCL) receives the input from photoreceptors via the bipolar and amacrine cells and transmit the signals to the brain. b Localization of selected genes associated with RDs in dogs. *Indicates the cone photoreceptors. Canine RD genes with unknown retinal localization or ubiquitous expression are not displayed. OS outer segment, IS inner segment. c The retinoid cycle recycles the light-absorbing chromophore. Absorption of a photon (hv) converts 11-cis-retinal bound to opsin (Rho) into all-trans-retinal initiating phototransduction. All-trans-retinal is reduced by photoreceptor retinol dehydrogenase (RDH) to all-trans-retinol and exported to the RPE where it is esterified by lecithin retinol acyltransferase (LRAT), converted to 11-cis-retinol by RPE65, and oxidized to 11-cis-retinal by NAD and a cis-specific retinol dehydrogenase (cis-RDH). 11-cis-retinal is exported back to the OS to again bind to opsin. CRALBP cellular retinaldehyde-binding protein, CRBP cellular retinol-binding protein, IRBP interphotoreceptor matrix retinoid-binding protein. d The phototransduction cascade converts light stimuli to electrical signal. Activated opsin (R*) in the disk membrane activates transducin (G) to G* which in turn activates phosphodies-terase (PDE) to PDE**. PDE** hydrolyzes cGMP, reducing its cytoplasmic concentration which results in closure of cGMP-gated channels in the plasma membrane. This causes hyperpolarization of the photoreceptors leading to signals sent to the downstream neurons. The schematics are modified and reprinted with permission from John Wiley and Sons (Nawrot et al. 2006) (c) and Elsevier Limited (Leskov et al. 2000; Pugh 1999) (d)

Two critical pathways involved in vision are the retinoid cycle (Fig. 2c) and phototransduction (Fig. 2d). The phototransduction cascade is triggered by the absorption of light photons and generates an amplified chemical signal. All photoreceptor OSs contain an opsin bound to a light-absorbing chromophore, 11-cis-retinal, the precursor of which is all-trans-retinol (vitamin A). The opsins in rods (rhodopsin) and cones (photopsin) differ significantly in their spectral sensitivity, i.e., the range of wavelengths to which they are most sensitive. The retinoid cycle recycles all-trans-retinol to 11-cis-retinal in the RPE and replenishes the chromophore for rod and cone opsin pigments. Mutations in genes participating in the activation or deactivation of phototransduction as well as the retinoid cycle are causally associated with RDs. Many of the known genes underlying canine retinal diseases code for proteins localized to the outer retina where these functions take place (Fig. 2b).

Genes associated with RDs in man and dogs

Although mutations in phototransduction or visual cycle genes have repeatedly been identified as causes of RDs, they represent but a small part of the suite of disease-associated genes that cause blindness. Human RDs are known for extensive phenotypic and genetic heterogeneities (Supplementary Table 1) resulting in disease categories such as RP, CRD, and LCA. RP is associated with initial night blindness and subsequent progressive loss of peripheral visual fields. In contrast, the cones and central vision are initially more affected in CRD, while LCA manifests in childhood, with severe visual impairment or total blindness. Syndromic forms of RD (e.g., sensorineural blindness and deafness represented by Usher syndrome disease class) involve mutations in genes common to ciliated sensory hair cells and photoreceptors (van Wijk et al. 2006, 2009). While canine RDs have been identified in the RP, CRD, and LCA disease classes, syndromic RDs have not been identified (Supplementary Table 1). It is possible that lack of recognition could result in the different expression of the auditory/vestibular and retinal phenotypes with mutations in Usher syndrome genes, as occurs in mice where mutations in almost all of the Usher syndrome genes cause vestibular and auditory abnormalities but minimal or no retinal phenotypes.

The clinical manifestations and the underlying genes vary substantially within each disease category. On the other hand, the same gene may be associated with multiple forms of RD showing genotypic as well as phenotypic overlap among them. For example, recessive mutations in GUCY2D cause LCA, whereas dominant mutations in the same gene result in CRD (Booij et al. 2005; Hunt et al. 2010). In accordance with the homology in the retinal system, almost all known canine genes underlying different forms of RD have human disease counterparts (outlined or underlined in Supplementary Table 1). Also, all the genes associated with human RDs are potential candidates for the equivalent canine condition.

Inherited retinal diseases in dogs: diverse phenotypic groups

Retinal diseases in dogs can be classified as progressive, stationary, or developmental (Table 1). While the majority of canine RDs are autosomal recessive, other forms of Mendelian inheritance are known: an autosomal dominant PRA (ADPRA) is known to be in the Bullmastiff and English mastiff dogs with a missense mutation in (RHO) gene (Kijas et al. 2002, 2003). X-linked forms of PRA in Samoyeds and Siberian Huskies (XLPRA1) and in mixed breeds (XLPRA2) (Acland et al. 1994; Zhang et al. 2002) are known to be caused by nonallelic mutations in the RPGR (retinitis pigmentosa GTPase regulator) gene.

Table 1. Examples of canine retinal diseases with clinical and/or molecular characterization.

Disease name	Disease symbol	Breed	Gene	Gene function	Mutation	Approach	References
Progressive disorders
Progressive retinal atrophy (PRA)
Rod–cone dysplasia 1	rcdl	Irish setter	PDE6B	Phototransduction	G2426A (W807X)	Candidate gene	Aguirre et al. 1978; Clements et al. 1993; Farber et al. 1992; Parry 1953a, b; Ray et al. 1994; Suber et al. 1993
Rod–cone dysplasia la	rcdla	Sloughi	PDE6B	Phototransduction	8-bp ins, exon 21	Candidate gene	Dekomien et al. 2000
Rod–cone dysplasia 2	rcd2	Collie	RD3	Phototransduction	22-bp ins, exon 4	LA (colony)	Acland et al. 1989; Kukekova et al. 2006, 2009; Woodford et al. 1982
Rod–cone dysplasia 3	rcd3	Cardigan Welsh corgi	PDE6A	Phototransduction	1-bp del, exon 15	Candidate gene	Petersen-Jones et al. 1999
Rod–cone dysplasia 4	rcd4	Gordon/Irish setter	–	–	–	GWAS (pet)	Mellersh, personal communication
Rod dysplasiaa	rd	Norwegian elkhound	N.D.	–	–	–	Aguirre 1978
Early retinal degeneration	erd	Norwegian elkhound	STK38L	N.D.	SINE ins, exon 4	LA (colony)	Acland and Aguirre 1987; Acland et al. 1989, 1999; Goldstein et al. 2010b
Progressive rod–cone degeneration	prcd	22 breedsb	PRCD	N.D.	G5A (C2Y)	LA (colony)	Acland et al. 1998; Aguirre and Acland 1988; Aguirre et al. 1982a; Goldstein et al. 2006; Sidjanin et al. 2003; Zangerl et al. 2006
Golden retriever PRA 1	GR_PRA1	Golden retriever	SLC4A3	Anion exchange	1-bp ins, exon 16	GWAS (pet)	Downs et al. 2011
X–linked PRA 1	XLPRA1	Samoyed, Siberian Husky	RPGR	Cilia trafficking	5-bp del, exon ORF15	LA (colony)	Acland et al. 1994; Zeiss et al. 1999; Zhang et al. 2000, 2001, 2002
X–linked PRA 2	XLPRA2	Mongrel	RPGR	Cilia trafficking	2-bp del, exon ORF15	LA (colony)	Zhang et al. 2002
Autosomal dominant PRA	ADPRA	Bullmastiff, English mastiff	RHO	Phototransduction	C147G (T4R)	Candidate gene	Kijas et al. 2002, 2003
Generalized PRAc	(gPRASPD)	Schapendoes	CCDC66	N.D.	1-bp ins, exon 6	LA (colony)	Dekomien et al. 2010; Lippmann et al. 2007
Cone–rod dystrophy (CRD)
Cone–rod dystrophy	crdl	American Staffordshire terrier	N.D.	–	–	–	Kijas et al. 2004
Cone-rod dystrophy	crd2	American pit bull terrieir	N.D.	–	–	–	Kijas et al. 2004
Cone–rod dystrophy	crd3	Glen of Imaal	ADAM9	N.D.	23-kb del (removal of exons 15-16)	GWAS (pet, colony)	Goldstein et al. 2010c; Kropatsch et al. 2010
Cone-rod dystrophy	cordl (crd4)	Miniature longhaired dachshund	RPGRIP1	Cilia trafficking	44-bp ins, exon 2	LA (colony) GWAS (pet)	Barnett 1965; Curtis and Barnett 1993; Mellersh et al. 2006; Miyadera et al. 2010; Turney et al. 2007
Cone–rod dystrophy	(CRDSWHD)	Standard wirehaired dachshund	NPHP4	Cilia trafficking	180-bp del, exon/intron 5	GWAS (colony)	Ropstad et al. 2007, 2008; Wiik et al. 2008
Stationary disorders
Achromatopsia (cone degeneration, hemeralopia)	cdAMAL	3 breedsd	CNGB3	Phototransduction	140-kb del (removal of all exons)	LA (colony)	Rubin et al. 1967; Sidjanin et al. 2002
Achromatopsia (cone degeneration, hemeralopia)	cdGSPT	German shorthaired pointer	CNGB3	Phototransduction	G808A (D262N)	LA (colony)	Sidjanin et al. 2002
Canine LCA (congenital stationary night blindness)	cLCA (csnb)	Briard	RPE65	Visual cycle	4-bp del, exon 5	Candidate gene	Aguirre et al. 1998; Narfström et al. 1989; Veske et al. 1999; Wrigstad et al. 1994
Developmental disorders
Oculoskeletal dysplasia 1	osdl (drdl)	Labrador retriever	COL9A3	Collagen formation	1-bp ins, exon 1	LA (colony)	Acland and Aguirre 1995; Carrig et al. 1988; Goldstein et al. 2010a
Oculoskeletal dysplasia 2	osd2 (drd2)	Samoyed	COL9A2	Collagen formation	1,267-bp del (removal of exon 1)	LA (colony)	Acland and Aguirre 1995; Goldstein et al. 2010a; Meyers et al. 1983
Collie eye anomaly	cea	9 breedse	NHEJ1	N.D.	7.8-kb del, intron 4	LA (colony)	Lowe et al. 2003; Parker et al. 2007
Others
Canine multifocal retinopathy 1	cmrl	9 breedsf	BEST1	Epithelial transport	C73T (R25X)	Candidate gene	Guziewicz et al. 2007
Canine multifocal retinopathy 2	cmr2	Coton de Tulear	BEST1	Epithelial transport	G482A (G161D)	Candidate gene	Guziewicz et al. 2007
Canine multifocal retinopathy 3	cmr3	Lapponian herder	BEST1	Epithelial transport	1-bp del, exon 10; G1466T (G489V)	Candidate gene	Zangerl et al. 2010

Disease symbols shown in parentheses are either alias symbols or diseases that the symbol names have not been described previously. The Approach column shows the method used to identify the chromosomal location and/or the gene and the type of sample population used

LA linkage analysis, N.D. not determined, GWAS genome-wide association study

^aThe original colony of rd dogs is extinct

^bBreeds affected with prcd: American cocker spaniel, American Eskimo dog, Australian cattle dog, Australian shepherd, Australian stumpy tail cattle dog, Chesapeake Bay retriever, Chinese crested, English cocker spaniel, Entlebucher mountain dog, Finnish Lapphund, Golden retriever, Karelian bear dog, Kuvasz, Labrador retriever, Lapponian herder, Norwegian elkhound, Poodle (miniature and toy), Nova Scotia duck tolling retriever, Portuguese water dog, Spanish water dog, Swedish Lapphund, Yorkshire terrier

^cGeneralized PRA (gPRA) is a collective term previously used to describe inherited diseases affecting the dog neural retina. This was used to differentiate the diseases from central PRA (cPRA) which is likely a nutritional defect and affects the RPE

^dBreeds affected with cd^AMAL: Alaskan Malamute, Miniature Australian shepherd, Siberian Husky

^eBreeds affected with cea: Australian shepherd, Boykin spaniel, Border collie, Lancashire heeler, Nova Scotia duck tolling retriever, Rough and Smooth collie, Shetland sheepdog, Silken windhound, Longhaired whippet

^fBreeds affected with cmrl: Australian shepherd, Cane Corso, Dogue de Bordeaux, Great Pyrenees, Bullmastiff, English mastiff, Perro de Presa Canario, and English and American bulldog

Progressive disorders

Progressive forms of canine RDs affect primarily the photoreceptor cells and are classified as PRA and CRD. The progressive designation is based on serial clinical and functional assessments that show a continuous loss of retinal structure over time accompanied by loss of electroretinogram (ERG) rod and/or cone function. The rate of progression is variable and gene/mutation- and breed-dependent.

PRA, homologous to RP in humans, is characterized by initial degeneration of rod photoreceptors. The mutations can affect rods only (e.g., PDE6B in rcd1), or both rods and cones (e.g., RPGR in XLPRA1 and 2), but earlier and more severe loss of rods is the salient PRA phenotype (Fig. 3a). Consequently, cone loss can be secondary and the result of the rod degeneration, or it can be a primary event that occurs later and progresses more slowly than the rod disease. The dominance of rod involvement in PRA causes night blindness, commonly the initial clinical finding that progresses to severe visual dysfunction under both dim and bright light. Associated with these defects, affected dogs show characteristic fundus changes that are visible with an ophthalmoscope—hyperreflectivity of the tapetum, attenuation of the retinal blood vessels, and pale optic discs. Some dogs may develop secondary cataracts in the late stages of the disease.

Fig. 3.

ERG of dogs affected with PRA and CRD. Rod, rod/cone, and cone ERG recordings obtained by dark-adapted responses to blue, red, and white flashes, respectively. Rod and cone flicker recordings were obtained by low- (5 Hz) and high-intensity [5 Hz (a), 30 Hz (b)] white flashes, respectively. Onset of stimuli for the rod and cone flicker responses are indicated by the upward deflection of square wave pulses below the responses (a) or short vertical arrows under the responses (b). a Absence of rod responses with subsequent and progressive deterioration of the cone function in a Norwegian elkhound affected with rd (reprinted from Aguirre 1978). b Cone dysfunction detected by 1.2 years, followed by further deterioration of both cone and rod function in Glen of Imaal terrier dogs affected with crd3. At all ages, the loss of cone function is more prominent than that for rods (reprinted with permission from Goldstein et al. 2010c)

In contrast, CRD is a disorder predominantly of cones, with rods being affected later or to a lesser extent, at least initially. Particularly in early-onset forms, this can cause the initial clinical signs to be defects in photopic (day) visual function. Many cases, however, are referred when there is already extensive impairment of visual function under both bright and dim light, and the ophthalmoscopic findings are similar to PRA. Hence, the identification of a cone–led abnormality relies on ERG testing at the early stage of disease pathology (Kijas et al. 2004; Turney et al. 2007) (Fig. 3b). For this reason, CRD may be indistinguishable from PRA at the clinical level, thus often being referred to as PRA, although it is a distinct disorder by itself. Most of the currently recognized CRD forms, e.g., crd1 in American Staffordshire terriers, crd2 in American pit bull terriers, cord1 (crd4) in Miniature longhaired dachshunds, and CRD in Standard wirehaired dachshunds, show extensive retinal degeneration before 1 year of age (Curtis and Barnett 1993; Kijas et al. 2004; Ropstad et al. 2007, 2008), while crd3 in Glen of Imaal terriers manifests later in life with slower progression (Goldstein et al. 2010c; Kropatsch et al. 2010).

Clinical studies of PRA/CRD have shown a broad age-of-onset range in various breeds. Although affected dogs show similar ophthalmoscopic abnormalities at the end stage of the disease, the age when the first changes become evident and the rate of progression vary, and is mostly breed-specific (Supplementary Table 2). Retinal dysfunction detected by reduction in ERG amplitudes has long been the only available tool to detect subclinical PRA/ CRD, as ophthalmoscopic abnormalities may not be detectable until at least 70% of the photoreceptor cells have been lost (Parshall et al. 1991).

Early-onset PRA/CRD manifests during the postnatal retinal differentiation period (2–6 weeks) and results from abnormal or arrested retinal development or progressive degeneration that commences during or immediately after retinogenesis. The early onset of these diseases typically results in relatively rapid progression toward end-stage retinal degeneration and can be clinically evident in young adult or preadult dogs (Supplementary Table 2).

In contrast, late-onset PRA/CRD is characterized by pathological changes that first become evident after normal development and maturation of the retina. As such, they represent defects in pathways critical for the long-term maintenance of normal photoreceptor function and viability. Visual–behavioral abnormalities may not become apparent until well after reproductive maturity. Typically, further progression toward end-stage retinal degeneration is much slower than in early-onset PRA/CRD and may not become clinically evident until very late in life.

Stationary disorders

In contrast to the progressive ophthalmoscopic changes and visual–behavioral problems observed in PRA/CRD, certain forms of RD show either extremely slow or no further abnormalities or deterioration in vision from the time of initial diagnosis in young animals. Both canine achromatopsia, previously known as cone degeneration and canine hemeralopia (Aguirre and Rubin 1974, 1975), in the Alaskan Malamute (cd^AMAL) and the German shorthaired pointer (cd^GSPT) (Sidjanin et al. 2003), and canine LCA (cLCA), previously known as congenital stationary night blindness (csnb) (Narfström et al. 1989) and retinal dystrophy (Wrigstad 1994) in the Briard, are essentially stationary disorders. Cones alone are affected in cd^AMAL and cd^GSPT because of a mutation in CNGB3, a cone–specific gene, whereas both rods and cones are dysfunctional in cLCA because a mutation in RPE65, a RPE-specific isomerohydrolase, impairs the retinoid cycle and 11-cis-retinal is not made (Fig. 2c). While the achromatopsia-affected retina shows no further deterioration with age in vision or retinal abnormalities, old cLCA-affected dogs show retinal thinning, both clinically and histologically, an indication that the stationary classification may be a misnomer.

Developmental disorders

Developmental disorders affect retinal development and are considered nondegenerative retinal diseases. Retinal dysplasia is a developmental disorder characterized by a defect in retinal differentiation. Nonsyndromic forms have been reported in several breeds [e.g., Labrador retrievers (Barnett et al. 1970) and Bedlington terriers (Rubin 1968)], but the underlying genetic causes are unknown. On the other hand, syndromic retinal dysplasia in Labrador retrievers (osd1) and Samoyeds (osd2) are nonallelic oculoskeletal disorders that model autosomal recessive Stickler syndrome in man; they are caused by mutations in COL9A3 and COL9A2, respectively, both of which code for components of the type IX collagen fibril (Goldstein et al. 2010a; Meyers et al. 1983; Nelson and MacMillan 1983).

Another developmental disorder is Collie eye anomaly (cea) which affects the retina–choroid–scleral complex and serves as a model for macular coloboma in man. Disease phenotype is variable, ranging from choroidal hypoplasia, scleral coloboma, and retinal detachment. Because of the high disease prevalence of cea in Collies on a worldwide basis (70–90%), it is the most common inherited canine retinal disorder, although most affected dogs are not visually impaired (Barnett and Stades 1979; Roberts 1969). Shared ancestry among Collie breeds, including the Rough and Smooth collie, Australian shepherd, Border collie, and Shetland sheepdog, allowed the fine mapping of a region in the canine chromosome 37 (CFA37) to a common disease haplotype, and identified a homozygous deletion in NHEJ1 that spans a highly conserved binding domain (Lowe et al. 2003; Parker et al. 2007).

Searching for genes and mutations that cause canine retinal degenerations

The approaches used to search for genes and mutations underlying inherited traits of the dog have evolved with the availability of canine genomic resources which initially were negligible; for the most part, no canine-specific resources were available prior to 1997. Initial attempts focused on candidate genes, with the shift to linkage mapping and positional cloning strategies (Fig. 4). More recently, the microarray-based genome-wide association study (GWAS) with SNPs has been used as it became available.

Fig. 4.

Discovery approaches for canine RD loci and genes. The cumulative numbers of loci and mutations identified as causing canine RD are shown, classified according to the discovery approach used. Note that the chromosomal location and the mutation were reported concurrently for cd, cord1, osd1, and osd2. The disease symbols correspond to those shown in Table 1

The initial tools for mapping were provided by the canine meiotic linkage map (Mellersh et al. 1997), dog– rodent somatic hybrid cell lines (Langston et al. 1997), low- and high-resolution radiation hybrid maps (Guyon et al. 2003; Priat et al. 1998) that were integrated with single-locus probes (Breen et al. 2001), and BAC library (Li et al. 1999). Following the publication of a 1.5× genome sequence of the poodle (Kirkness et al. 2003), the 7.5× sequence of the Boxer dog became available in 2005 along with a dense map of single nucleotide polymorphisms (SNPs) across breeds (Lindblad-Toh et al. 2005).

Among the different canine diseases, RDs may have had the most comprehensive variety of approaches used to identify the underlying genes and mutations. The approaches developed and tested are described below in chronological order.

Candidate gene analysis

Early studies relied on a candidate gene approach largely because of the lack of alternative canine genomic tools. Candidate genes were most commonly selected for evaluation based on a priori knowledge of the biochemical pathway or defect involved, or on information from other species such as humans and mice. Novel candidates were also identified by the use of subtraction techniques to clone genes differentially expressed between normal and mutant retinas (Zangerl et al. 2002; Zhang et al. 1998).

The first mutation responsible for PRA was found by screening PDE6B as a candidate gene for rcd1 in Irish setters (Clements et al. 1993; Suber et al. 1993), because this canine disease was phenotypically and biochemically similar to rd in mice resulting from a PDE6B mutation (Bowes et al. 1990; Farber and Lolley 1974). In rcd1, the activity of retinal cyclic guanosine monophosphate phosphodiesterase (cGMP-PDE) was impaired (Aguirre et al. 1978, 1982b), and the mRNA levels for PDE6B were selectively decreased as in rd mice (Farber et al. 1992). Sequencing of canine PDE6B identified a stop mutation in exon 21 (Clements et al. 1993; Ray et al. 1994; Suber et al. 1993). A candidate gene approach was also used to successfully identify a different mutation in PDE6B for rcd1a in the Sloughi (Dekomien et al. 2000), PDE6A for rcd3 in the Cardigan Welsh corgi (Petersen-Jones et al. 1999), and RHO for ADPRA in the Bullmastiff and English mastiff (Kijas et al. 2002).

However, many canine RDs were tested for mutations in many different candidate genes related to retinal structure or function without success. Examples include PDE6A (Wang et al. 1999), PDE6D and PDE6G (Dekomien and Epplen 2003; Wang et al. 1999), arrestin (Dekomien and Epplen 2002c), peripherin/rds (Ray et al. 1996; Runte et al. 2000), ROM1 (Gould et al. 1997; Klein et al. 1998), phosducin (Dekomien and Epplen 2002a; Lin et al. 1998; Zhang et al. 1998), opsin (Gould et al. 1995; Ray et al. 1999), and recoverin (Dekomien and Epplen 2002b). As mutations could be identified in only 3.4% of the candidate gene studies of canine RDs, there was considerable pessimism as to the applicability and efficacy of this approach (Aguirre-Hernandez and Sargan 2005). Given that many negative results for these studies may have never been published, the success rate could be even lower.

Nonetheless, the “classic” candidate gene approach has recently proven successful in identifying the mutations in BEST1 associated with cmr1, cmr2, and cmr3 where a phenotype-directed candidate gene approach was used (Guziewicz et al. 2007; Zangerl et al. 2010). Canine multifocal retinopathy (cmr) shows initial multifocal areas of retinal elevation that closely resemble those of the vitelliform or yolk-like lesion in Best macular dystrophy (BMD) human patients. Both canine and human forms eventually progress into retinal atrophy with concomitant histopathologic RPE abnormalities. Screening of BEST1, known to be associated with BMD, promptly identified mutations in cmr dogs, underscoring the clinical, pathologic, and molecular resemblances between the homologous canine and human diseases.

Linkage mapping and positional cloning strategies

Unlike candidate gene studies, which limit the genomic region of inspection, a whole-genome linkage analysis (LA) mapping interrogates the entire genome, including unknown genes, using informative families containing affected and unaffected dogs. Cosegregation of genetic markers and phenotype is analyzed to identify recombination, or lack thereof, between the disease phenotype and marker alleles. Subsequently, the region of interest is fine-mapped using additional markers to increase the resolution, and positional candidate genes in the narrowed interval are analyzed.

To date, 11 chromosomal loci have been mapped by LA leading to the identification of 12 mutations corresponding to different forms of RD. In many cases, identification of the actual gene/mutation took some time after mapping of the chromosomal location. This was due to the long linkage disequilibrium (LD) that had facilitated the mapping process but then ironically hindered narrowing of the critical interval and the number of genes within. One example is the prcd-PRA locus mapped on CFA9 using multiple informative pedigrees (Acland et al. 1998). Genetic markers linked to the disease-causing mutation in the mapped interval were used to perform a “linked” DNA test until the corresponding mutation was identified by positional cloning of a novel gene PRCD (Zangerl et al. 2006).

In LA mapping, large and complete multigenerational canine pedigrees with phenotypic data are required. In theory, the dog is suitable for LA mapping in that detailed pedigree records, large-sized families, and condensed life span facilitate sample collection from multiple generations. In practice, however, accessing the sample population to ascertain phenotypes and eliminate this variable, together with access to all necessary DNA samples, is often a daunting task. For that reason, many of the mapping studies have relied on purpose-bred research colonies developed from naturally occurring cases and expanded by back-crossing and/or outcrossing. Not only can the phenotype be ascertained using multiple testing modalities, but samples from the target and other tissues are readily obtained for analysis to validate gene expression, positional candidates, and phenotype–genotype correlation (for review see Aguirre and Acland 2006).

Genome-wide association studies

As part of the project that developed the 7.5× canine genome reference sequence, over 2.5 million SNPs were identified across the canine genome (Lindblad-Toh et al. 2005). These markers are more abundant than microsatellites and sufficiently densely and broadly placed across the genome to enable association studies. Furthermore, genotyping microarrays have become available, making high-throughput SNP analysis feasible in both humans and domestic animals (Andersson 2009). For the dog, SNP platforms that yield 22,362 (22 k) (Illumina), 49,663 (50 k) (Affymetrix), 127 k (Affymetrix), and 170 k (Illumina) SNP genotypes are available.

A decided advantage of case-control GWAS over LA is that the former does not necessarily require the large, multigenerational pedigrees which, in most cases, require purpose-bred research colonies. Based on the long within-breed LD (0.5–5 Mb, depending on the breed) (Gray et al. 2009; Sutter et al. 2004), 10,000–30,000 SNPs are considered sufficient to map a Mendelian trait with high penetrance and no phenocopies. With this marker density, 20 cases and 20 controls and 50 cases and 50 controls are considered more than sufficient to map autosomal recessive and dominant traits, respectively (Lindblad-Toh et al. 2005).

Since the early successful GWAS, which identified the hair ridge locus in Rhodesian ridgebacks (Karlsson et al. 2007; Salmon Hillbertz et al. 2007) and the white spotting locus in Boxers and other breeds (Karlsson et al. 2007), over 25 genetic traits ranging from ocular (Kuchtey et al. 2011), neurologic (Awano et al. 2009; Drogemuller et al. 2010; Farias et al. 2011; Zeng et al. 2011), dermatologic/immunologic (Drogemuller et al. 2008; Olsson et al. 2011; Wang et al. 2011; Wilbe et al. 2010; Wood et al. 2009), orthopedic (Drogemuller et al. 2009; Parker et al. 2009; Zhou et al. 2010), cardiologic (Meurs et al. 2010), and behavioral disorders (Dodman et al. 2010) to morphology (Bannasch et al. 2010; Boyko et al. 2010) and coat types (Cadieu et al. 2009) have been mapped by GWAS. For canine RDs, three disease forms have been mapped by GWAS to date validating this approach.

Autosomal recessive CRD in the Standard wirehaired dachshund was mapped using an array-based genome-wide sibling transmission disequilibrium test (sibTDT) approach with 13 discordant sib-pairs from a purpose-bred research colony (Wiik et al. 2008). Using 50 k SNP chips, the CRD^SWHD locus was mapped to a 6.5-Mb interval on CFA5 containing <70 genes. Fine mapping using 36 informative offspring could not narrow the critical interval. Resequencing of positional candidate genes identified a 180-bp deletion in exon/intron 5 of NPHP4 (nephronophthisis 4). RNA analysis of NPHP4 showed that skipping exon 5 resulted in a truncated protein lacking the domain interacting with RPGRIP1 in the retina but retention of the domain interacting with NPHP1 in the kidney. As mutations in NPHP4 had been associated with simultaneous eye and kidney disease in humans and mice (Mollet et al. 2002; Otto et al. 2002), the lack of the RPGRIP1-interacting domain and retention of the NPHP1-interacting domain explain the retina-specific clinical signs in CRD^SWHD.

A second example is the autosomal recessive crd3 in the Glen of Imaal terrier (Goldstein et al. 2010c). Mostly client-owned and chosen to be the least related, 20 affected cases and 22 unaffected controls were selected for a case-control GWAS using 127 k SNP chips. The crd3 locus was mapped to a 2.74-Mb region of homozygosity on CFA16. Sequencing of positional candidate genes identified a large genomic deletion of approximately 23 kb, including exons 15 and 16 of ADAM9. The identification of an ADAM9 mutation established crd3 as the canine ortholog of CORD9, a form of human CRD (Parry et al. 2009).

The most recent demonstration of GWAS in a canine RD was GR_PRA1 in the Golden retriever (GR) (Downs et al. 2011). RD caused by the canine prcd mutation is known to segregate in GRs, but identification of PRA-affected cases lacking the prcd mutation prompted an association study. GWAS using 22 k SNPs on 27 cases (RD-affected, non-prcd) and 19 controls from the GR pet population mapped the GR_PRA1 locus to a 644-kb interval of homozygosity on CFA37. Sequencing positional candidate genes identified a 1-bp insertion in SLC4A3 (solute carrier family 4, anion exchanger, member 3) that resulted in a premature stop codon. Although the mutation cosegregated with the PRA phenotype in certain lineages with autosomal recessive mode of inheritance, there was substantial genotype–phenotype discordance in the overall GR population studied suggesting the presence of one or more additional PRA loci. Although SLC4A3 seems to have a role in retinal function, it has not previously been associated with RD in human patients or naturally occurring mouse strains, making GR_PRA1 a unique model for further investigation.

Effective use of isolated subpopulations

prcd in different breed populations

The current approach of choice to identify the genes and the mutations underlying canine RDs is a SNP chip-based GWAS followed by fine mapping and sequencing of positional candidates. However, narrowing of the mapped critical region to a manageable interval remains challenging. While the extensive within-breed LD facilitates initial mapping of a chromosomal region, moving to the target gene may be hindered by the same phenomenon, with LD extending for megabases and spanning over 100 genes. The prcd locus was initially mapped by LA to a 1.9-Mb interval on CFA9 and contained about 50 genes. Identification of rare recombinant dogs and generation of a unique disease-specific haplotype using SNPs from BAC clones reduced the disease interval. Moreover, as the disease was shown to be present in many different breeds, ancestral recombinations were identified in different breeds (i.e., genetically isolated populations) to establish a narrower disease-specific LD region. Fine mapping eventually narrowed the LD interval to 87 kb containing three genes (Goldstein et al. 2006) leading to the identification of a point mutation in a novel gene, PRCD (Zangerl et al. 2006). This effort was complemented by a parallel approach, exploiting retinal expressed sequence tag (EST) characterization that mapped an EST clone to the disease interval (Zangerl et al. 2009) that proved to be part of the PRCD transcript.

cord1 in miniature longhaired dachshunds: differences in research and pet populations

Even if a particular form of RD is apparently restricted to a single breed, artificially and/or geographically isolated subpopulations of that breed can be exploited to aid in genetic analyses. The autosomal recessive, early-onset (<1 year) form of RD in Miniature longhaired dachshunds (MLHDs) was characterized using a research colony developed in the UK (Curtis and Barnett 1993). The disease was subsequently shown to be CRD and termed cord1 (cone–rod dystrophy 1) (Mellersh et al. 2006; Turney et al. 2007).

Affected cases and obligate carriers from the research colony were used to map the cord1 locus, and a whole-genome scan based on homozygosity and subsequent LA identified a 14.15-Mb interval on CFA15 (Mellersh et al. 2006). The human syntenic region contained a strong candidate gene, RPGRIP1 (RPGR-interacting protein 1), which had been associated with LCA and CRD in humans (Dryja et al. 2001; Gerber et al. 2001; Hameed et al. 2003). Sequencing of RPGRIP1 revealed a homozygous 44-bp insertion (RPGRIP1^−/−) in affected dogs resulting in a frameshift, and a premature stop codon that truncated most of the protein's functional domain. The mutation segregated completely with cord1 in the research colony.

Meanwhile, Miniature dachshunds had enjoyed an explosive popularity in Japan, with over one million new registrations between 1999 and 2008 according to the Japan Kennel Club. The sudden growth of the breed's population from a relatively small number of founders led to frequent manifestation of recessive diseases, including RDs. When samples from MLHD with RD collected in Japan were examined, it was found that the age of onset ranged broadly from 4 months to 15 years (mean ± SD = 4.6 ± 3.4 years), a striking difference from the UK research dogs that showed a uniform age of onset (Miyadera et al. 2009). There was also significant discordance between the retinal phenotype and the RPGRIP1 genotype in the broader MLHD population: 20% of blind cases were not RPGRIP1^−/−, while 16% of the apparently normal dogs were, in fact, homozygous mutant for the 44-bp insertion. Nonetheless, a significantly reduced cone ERG response was observed in RPGRIP1^−/− pet dogs that were clinically normal (Busse et al. 2011), suggesting that the locus mapped in the original research colony could still be pathogenic in the pet population but with a phenotype that was milder or not clinically apparent in some individuals.

To account for the genotype–phenotype discordance in the Japanese pet MLHDs, possible involvement of additional genetic loci was explored. GWAS using 80 RPGRIP1^−/− dogs showing variable phenotypes was performed. A single modifier locus that was homozygous in almost all the early-onset cases was identified and is now under investigation (Miyadera et al. 2012).

It seems likely that the dogs in the UK research colony were all homozygous or “fixed” for the second locus mapped using the Japanese pet dogs. Hence, only by studying the more genetically and clinically heterogeneous pet population could the second locus which modifies the disease onset be identified. In hindsight, the two-step mapping utilizing different subpopulations of MLHD facilitated the identification of two loci involved in cord1.

Dissecting the heterogeneous nature of canine retinal degenerations

Two aspects of genetic heterogeneity among canine RDs have recently emerged. In one situation, breed-specific phenotypes of canine RDs have been shown to arise from the same gene/mutation in different breeds. This is particularly evident in the breed-specific age of onset and rate of progression of the clinical phenotype and is broadly presumed to result from differences of breed-specific “genetic background.” On the other hand, some RDs that are phenotypically similar or even indistinguishable can result from distinct mutations in different genes in different populations. Research in our laboratories and by colleagues elsewhere has shown that both situations occur.

Phenotypic heterogeneity: variable clinical features

Intrafamilial variability has been observed among human RP patients harboring the same mutation (Berson et al. 1991). In contrast to most human populations, dogs have a more uniform genetic background which contributes to consistent disease phenotypes within a breed. However, some canine RD forms are known for substantial phenotypic variability among affected dogs of the same breed.

Among the affected male dogs from the XLPRA1 research colony, extensive variations in the age of onset and disease severity were observed (Zeiss et al. 1999). Because the affected dogs were known to harbor a stable, common mutant RPGR allele, the heterogeneous genetic background due to outcrossing was considered responsible for the phenotypic variation (Zeiss et al. 1999). Haplotypes of six candidate genes known to interact with RPGR or RPGRIP1 were examined for disease-severity association, but no gene-specific haplotypes were found to cosegregate with the moderate or severe phenotype. Also, no significant difference in retinal expression levels of the candidate genes was observed between normal and affected dogs (Guyon et al. 2007). The search for the modifying locus (loci) of XLPRA1 is ongoing.

Significant interbreed variation in the age of onset has been seen among the multiple breeds affected with prcd. The within-breed phenotype is uniform in most affected breeds, and the breed-characteristic age of clinical disease onset ranges from 3 to 12 years (Supplementary Table 2). However, in prcd-affected American Eskimo dog, Nova Scotia duck tolling retriever, Australian cattle dog, and English cocker spaniel, the age of onset varies extensively among PRCD^−/− dogs of the same breed. Based on the variable phenotypes observed among affected dogs in a common research facility, genetic rather than environmental factors appear to be the major modifiers.

Variable ages of onset and disease severity has been observed in the ADPRA-affected English mastiffs with a mutation in RHO (Kijas et al. 2002, 2003). In this case, environmental factors such as exposure to light were identified as modifiers, as the mutation that abolished the first consensus glycosylation site rendered the chromophore-free mutant protein extremely unstable (Cideciyan et al. 2005; Gu et al. 2007; Zhu et al. 2004).

Allelic heterogeneity: one gene, different mutations

Different RD forms may arise from different mutations in the same gene, a phenomenon termed allelic heterogeneity. The earliest example in canine RDs is the point mutation and an 8-bp insertion mutation each in exon 21 of PDE6B causing rcd1 and rcd1a, respectively (Dekomien et al. 2000; Suber et al. 1993).

Two distinct forms of X-linked PRA show marked allelic heterogeneity with mutation-specific phenotypes (Zhang et al. 2002). The 5-bp deletion in RPGRORF15 present in XLPRA1 results in a premature stop and truncation of the C-terminus of the protein. The mutation affects only the photoreceptors, and these degenerate in young adults after developing and functioning normally (Zeiss et al. 1999). In contrast, XLPRA2 is an early-onset developmental disease resulting from a 2-bp deletion of RPGRORF15. This deletion causes a frameshift and replacement of a glutamic acid-rich domain by arginine residues before truncation of the C-terminal end of the protein (Zhang et al. 2002). This leads to a toxic gain of function that severely compromises the photoreceptors in the early stages of postnatal development (Beltran et al. 2006).

Allelic heterogeneity is also found in canine achromatopsia and canine multifocal retinopathy. In the former, a 140-kb deletion and a missense mutation in CNGB3 occur in Alaskan Malamutes (cd^AMAL), and in German shorthaired pointers (cd^GSPT), respectively. The two forms are allelic, and a dog heterozygous for cd^AMAL and cd^GSPT was found to be achromatopsic (Sidjanin et al. 2002). More recently, distinct breed-specific mutations in the BEST1 gene have been associated with the cmr1, cmr2, and cmr3 forms of canine multifocal retinopathy (Guziewicz et al. 2007; Zangerl et al. 2010).

For some autosomal recessive canine disorders with multiple identified allelic mutations (e.g., rcd1, rcd1a, achromatopsia, and cmr), the phenotype resulting from each allelic series is highly consistent (i.e., regardless of the specific mutant allele), suggesting that the mutations compromise critical functional domains, and the phenotypes represent a loss of function. In contrast, the XLPRA phenotypes represent, in one case, a loss and, in the other case, a toxic gain of function resulting from changes in the size and/or charge of the ORF15 domain, and the phenotypes are markedly different. We would anticipate that yet-to-be-recognized mutations in canine RHO that do not affect consensus glycosylation sites similarly would result in very different phenotypes as in humans (Berson et al. 1991).

Allelism of autosomal recessive forms of early- and late-onset PRA in different breeds has been tested by interbreed crosses. Production of normal progeny from matings among rcd1, rcd2, and erd-affected dogs confirmed non-allelism of these diseases (Acland et al. 1989; Wang et al. 1999). Similarly, nonallelism between erd and prcd (Ray et al. 1996) and rd and prcd (Aguirre 1976) was established. In contrast, allelism for prcd in multiple breeds was demonstrated and was instrumental in LA and positional cloning of the novel retinal disease gene (Aguirre and Acland 1988, 2006).

Genetic heterogeneity: one breed, different forms of RDs

Often, only one form of RD caused by a single mutation segregates in a breed leading to a uniform phenotype. However, in some breeds, more than one form seems to be present (Table 2). Although we suspect that these represent cases of genetic heterogeneity based on molecular testing of known mutations in retinal disease genes, the possibility of allelic heterogeneity cannot be excluded until the causative mutations are identified or time-consuming cross-breeding studies are carried out.

Table 2. Examples of breeds affected with more than one form of retinal disease.

Breed	Disease 1	Disease 2	Disease 3	Disease 4
Irish setter	rcd1	rcd4	N.D. (late-onset)	–
Australian shepherd	prcd	cea	cmr1	cdAMAL
Golden retriever	prcd	GR_PRA1	N.D. (late-onset)	–
Lapponian herder	prcd	cmr3	Non-prcd PRA	–
Norwegian elkhound	prcd	rd	erd	–
Labrador retriever	prcd	osd1	–	–
Australian (stumpy tail) cattle dog	prcd	N.D.	–	–
Chinese crested	prcd	N.D.	–	–
Nova Scotia duck tolling retriever	prcd	cea	–	–
Toy/Miniature poodle	prcd	N.D.	–	–
Gordon setter	rcd4	N.D.	–	–
Miniature longhaired dachshund	cord1	N.D.	–	–
Bullmastiff/English mastiff	ADPRA	cmr1	–	–
Samoyed	XLPRA1	osd2	–	–
Shetland sheepdog	cea	SPRa	–	–

N.D. presence of at least one extra form of RD of which the disease form has not been determined

^aSPR (slowly progressive retinopathy) is a recently described rod–cone degeneration with/without ERG abnormality and showing ophthalmoscopic changes similar to the early stages of PRA. Visual impairment may not be obvious. prcd has been excluded by molecular testing in a subset of affected dogs (Karlstam et al. 2011)

For example, of the more than 22 breeds affected with prcd, Australian cattle dog, Australian stumpy tail cattle dog, Chinese crested, Golden retriever (GR), Labrador retriever, Norwegian elkhound, and Miniature/Toy poodle are now known to each segregate another (non-prcd) form of PRA which remains to be characterized molecularly (Optigen, http://www.optigen.com). In some breeds, DNA tests are currently available for two forms of RDs: examples include prcd and GR_PRA1 in GR, rcd1 and rcd4 in Irish setters, ADPRA and cmr in English mastiff, prcd and osd1 in Labrador retrievers, and XLPRA1 and osd2 in Samoyeds.

Common founder: one disease, multiple breeds

Many canine RDs are breed-specific and each RD is known to segregate in only one breed. However, some RDs are more broadly distributed, caused by a single mutant allele that is identical by descent (IBD). In some cases, breeds segregating an RD allele that is IBD share well-recognized recent common ancestry origin or have experienced interbreed gene flow during breed establishment. In other cases, however, the common ancestry is less obvious whether it is recent or much more ancient.

For example, the BEST1 stop mutation responsible for cmr1 occurs in several Molossus-type (Mastiff-derived) breeds, including the English mastiff, Bullmastiff, Great Pyrenees (Guziewicz et al. 2007), Cane Corso, Dogue de Bordeaux, English and American bulldogs, and Perro de Presa Canario (Guziewicz and Zangerl, personal communication) (Supplementary Fig. 1). Based on historical and morphological relatedness, IBD is easily understood and may represent descent from a relatively recent common ancestor. However, cmr1 phenotypes with the mutation identical to that in the Mastiff breeds was recently identified in the Australian shepherd (Hoffmann, Guziewicz, and Zangerl, personal communication), a breed considered to be genetically distant from the Mastiff breeds (Supplementary Fig. 1). Ongoing studies are exploring further the ancestry and possible gene flow regarding this outlier.

In the case of cea, an identical disease haplotype segregates not only in several Collie-related herding breeds with historic common ancestry, but also in less obviously related nonherding breeds (e.g., Boykin spaniel and Nova Scotia duck tolling retriever), suggesting the possibility of past or recent gene flow from the herding group.

A situation similar to that for cea pertains to canine achromatopsia with an IBD cd^AMAL allele segregating in the Alaskan Malamute, Siberian Husky, and Miniature Australian shepherd breeds (Goldstein, personal communication; Yeh and Komaromy, personal communication), suggesting again either a much more ancient origin of the mutant allele or a more recent but unsuspected gene flow between breeds.

In contrast, the IBD mutant PRCD allele is shared by at least 22 breeds of very diverse origin (Fig. 5). It is likely to be an ancient mutation arising in a common founder before the breeds became isolated breeding populations. This founder mutation is not recognized to segregate in the cluster of “ancient” breeds that trace their ancestry to Asia or Africa (e.g., Akita, Chinese shar-pei, Siberian Husky, Basenji, or Afghan hound), emphasizing the distinctly more ancient origin of these breeds (Parker et al. 2004; Vonholdt et al. 2010).

Fig. 5.

Diverse breeds share a common identical-by-descent mutation in PRCD. Genetic diversity of 17 of 22 dog breeds that segregate the C2Y mutation in PRCD is shown in a neighbor-joining tree of domestic dogs and gray wolves (reprinted and modified with permission from Vonholdt et al. 2010). The names of breeds that were included in the study by Vonholdt et al. (2010) are outlined. For other breeds, images are placed at a hypothetical position based on breed history and previously reported structural analysis of canine breeds (Parker et al. 2004). Dog images are from Wikipedia (http://en.wikipedia.org/) and not to scale

Phenocopies: nongenetic retinopathies

Acquired forms of RDs such as sudden acquired retinal degeneration (SARD) may complicate the diagnosis and ascertainment of RDs (Acland et al. 1984; Cullen and Grahn 2002; Keller et al. 2006; Miller et al. 1998; O'Toole et al. 1992; Vainisi et al. 1983; van der Woerdt et al. 1991). SARD appears clinically similar to PRA/CRD except that the onset of total blindness is sudden whereas the vision loss is progressive in PRA/CRD. To clearly differentiate SARD from PRA/CRD, it is essential that examination be done at the onset of blindness by ophthalmoscopy (normal fundus) and ERG testing (nonrecordable ERG). At the end stage, both disorders are indistinguishable.

DNA testing

Almost all mutations that underlie RDs can be screened for by tests that examine a DNA sample for the presence or the absence of an RD mutation known or suspected to be segregating in the respective breed. Because of extensive genetic and allelic heterogeneities, molecular diagnoses of sporadic human RD patients requires screening of hundreds of genes and thousands of mutations, often using micro-array chips (Stone 2007; Zernant et al. 2005). In contrast, DNA testing of only the known mutation(s) associated with the respective canine breed is often sufficient for the molecular diagnosis of RDs.

A DNA test has advantages over other clinical diagnostic methods in that it can detect unaffected carriers as well as subclinical cases prior to the onset of disease. This allows selection of healthy reproductive pairs suitable for breeding. However, if there are other unidentified mutations causing other forms of RD in the breed, the currently available DNA test alone will not be able to exclude all forms of inherited RD in the breed.

Even when there is good correlation between the DNA test result and the retinal phenotype, a broader perspective is needed when applying the DNA test results to breeding programs. Nonretinal traits that cannot yet be tested for molecularly should also be taken into account to assess the overall health and genetic potential of the dog. Conservation of the within-breed genetic diversity also requires consideration when there is selective pressure by DNA testing, particularly if the mutation frequency is high. A sound breeding strategy will aim to prevent producing affected offspring and more gradually eliminate the RD mutant allele from the population, while at the same time maintaining the genetic diversity of a breed.

Prospects of canine retinal disease research

Study population

More than a dozen of canine RD research colonies have been developed for mapping, phenotype characterization, investigation of the disease pathology, and for gene or other therapies. While successful mapping of many diseases has used purpose-bred research colonies, some studies benefitted from recruiting cases and controls from client-owned pet dogs. Using samples and clinical data from client-owned dogs, the chromosomal location of prcd in the American Eskimo and Finish Lapphund breeds was independently mapped by homozygosity mapping using only 13 (Moody et al. 2005) and 12 (Aguirre-Hernandez et al. 2007) affected cases, respectively. The use of pet dogs eliminates the difficulties and expense of developing and maintaining a research colony. Sporadic cases from the pet population are ideal for case–control association studies as unrelated cases and controls will be studied without requiring prior assumptions about the mode of inheritance.

The drawbacks of studying pet dogs are the difficulties of collecting enough cases and suitable controls, particularly when the mutant allele frequency in the population is low. Also, the use of pet dogs may not be ideal for LA mapping where sufficiently large and informative pedigrees are required. Furthermore, as consistent diagnostic criteria are crucial for distinguishing different forms of RDs, standardization of phenotyping remains a challenge. Our experience in searching for the prcd locus defect indicated that approximately one third of the clinical records received were incomplete or inaccurate and could not be used in the homozygosity mapping studies used to reduce the critical interval. This problem is more serious when dealing with late- versus early-onset diseases because acquired retinal abnormalities are more common in older dogs and are a confounding variable.

Provided that rigorous phenotype ascertainment is obtained, the study population for GWAS can originate from the general pet population segregating the trait of interest, provided that there is sufficient cooperation of the owners, breeders, and referring veterinary clinicians. On the other hand, research colonies continue to play a critical role in providing adequate tissue resources to functionally evaluate positional candidate genes or changes in the DNA sequence.

Man to dog—dog to man

At least 24 mutations in 18 genes underlying canine RDs have been identified to date (Table 1). If we extrapolate from the human disease data, there may be more than 214 genes and loci critical for retinal function (Fig. 1) (Supplementary Table 1). While mutations in some of the human RD genes may not be associated with a recognizable phenotype in dogs (e.g., mutations in cone opsin genes that cause color vision deficits in humans without affecting visual acuity) (Nathans et al. 1986a, b), mutations in the majority of these genes would be expected to be associated with a recognizable phenotype in dogs.

On the other hand, genetic and pathologic findings derived from studies of canine RDs have contributed to the understanding of human disease. PRCD associated with prcd in multiple breeds was a novel gene first identified in dogs (Zangerl et al. 2006) and subsequently in humans. Strikingly, this led to the identification of an RP patient homozygous for the same G5A (C2Y) mutation that causes prcd in dogs, as well as other mutations in this gene (Nevet et al. 2010). Other examples of RD genes first described in the dog are CCDC66 and SLC4A3 underlying gPRA^SPD (Dekomien et al. 2010) and GR_PRA1 (Downs et al. 2011), respectively. Both loci were mapped by GWAS without presumption of the genes involved. CCDC66 was subsequently disrupted in a mouse model whose progressive rod–cone dysplasia phenotype reinforced the finding in the Schapendoes dog (Gerding et al. 2011).

Gene therapy

Identification of the genes and mutations underlying canine RDs have provided an excellent testing ground for gene replacement therapy, one of the approaches under active development to prevent, stabilize, or reverse the retinal degenerative process in man and animals.

Naturally occurring cLCA in Briards with a mutation in RPE65 has been treated successfully with subretinal injection of a recombinant adeno-associated virus 2 carrying wild-type RPE65 (Acland et al. 2001; Narfström et al. 2003). The single injection was safe, effective, and stable, with functional improvement up to 4 years post injection (Acland et al. 2005; Narfström et al. 2008). The treatment was subsequently applied to human LCA patients carrying RPE65 mutations and several trials have achieved significant restoration of visual function (Bainbridge et al. 2008; Hauswirth et al. 2008; Maguire et al. 2008).

Successful gene therapy using the dog model has also been achieved in CNGB3 achromatopsia (cd^AMAL, cd^GSPT) (Komaromy et al. 2010). Therapy directed specifically to cone photoreceptors led to the restoration of ERG cone function, day vision, and preservation of structure. Treatment was stable for the nearly 3-year study duration.

Conclusion

Since the first identification of the PDE6B mutation in 1993, there has been significant progress in the molecular investigation of canine RDs, benefiting from the rich genetic resources and tools developed over the years. Completion of the canine reference genome in 2005 further revolutionized the process of searching for genes and mutations underlying these and other diseases in dogs.

Canine RDs are true disease homologs for specific human disorders based on the phenotypic similarity and the common underlying gene defects, providing excellent models for analysis of the molecular mechanisms of these disorders. The number of canine RDs characterized at the molecular level will increase even further over the coming years, with GWAS being the current tool of choice and with rapid cost-effective whole-genome sequencing looming on the horizon.

Canine RDs have also been developed as an excellent model system to develop and test novel therapies applicable to humans. Because of the similarities in disease phenotype and the similar structure and function of the canine and human retinas, dogs are ideally suited for developing these therapies. Thus, the transition from gene discovery to gene therapy leading to the application to human medicine has been made possible.

The unique phenotypic and genetic uniformity as well as variability within and across dog breeds has been the key to the dramatic progress in the molecular understanding of RDs in dogs and will continue to be helpful in discovering retinal and other diseases in the future.