Abstract
Glucose dehydrogenase (GO) was found to be expressed during the pupal stage in both sexes of Drosophila melanogaster, but is limited to the male ejaculatory duct at the adult stage. During copulation GO is transferred from males to females. Mutational analysis of the Go locus indicates that a single structural gene encodes the pupal and ejaculatory duct GO. Thus an example of an enzyme structural gene switching from non-sex-limited to sex-limited expression has been found. Go mutants are recessive lethals exhibiting a late pupal effective lethal phase. These mutants can be rescued by excising the anterior end of the pupal case 0-2 days prior to the normal adult emergence time. It appears that the function of GO in pupae is to aid in the degradation of the puparium cuticle in preparation for the eclosion of the adult.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bak T. G. Studies on glucose dehydrogenase of Aspergillus oryzae. II. Purification and physical and chemical properties. Biochim Biophys Acta. 1967 Jul 11;139(2):277–293. doi: 10.1016/0005-2744(67)90032-0. [DOI] [PubMed] [Google Scholar]
- Cavener D. R., Clegg M. T. Temporal Stability of Allozyme Frequencies in a Natural Population of DROSOPHILA MELANOGASTER. Genetics. 1981 Jul;98(3):613–623. doi: 10.1093/genetics/98.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cavener D. R. Genetics of male-specific glucose oxidase and the identification of other unusual hexose enzymes in Drosophila melanogaster. Biochem Genet. 1980 Oct;18(9-10):929–937. doi: 10.1007/BF00500125. [DOI] [PubMed] [Google Scholar]
- Katzen H. M., Soderman D. D., Cirillo V. J. Tissue distribution and physiological significance of multiple forms of hexokinase. Ann N Y Acad Sci. 1968 Jun 14;151(1):351–358. doi: 10.1111/j.1749-6632.1968.tb11900.x. [DOI] [PubMed] [Google Scholar]
- Lewis R. A., Kaufman T. C., Denell R. E., Tallerico P. Genetic Analysis of the Antennapedia Gene Complex (Ant-C) and Adjacent Chromosomal Regions of DROSOPHILA MELANOGASTER. I. Polytene Chromosome Segments 84b-D. Genetics. 1980 Jun;95(2):367–381. doi: 10.1093/genetics/95.2.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madhavan K., Fox D. J., Ursprung H. Developmental genetics of hexokinase isozymes in Drosophila melanogaster. J Insect Physiol. 1972 Aug;18(8):1523–1530. doi: 10.1016/0022-1910(72)90231-4. [DOI] [PubMed] [Google Scholar]
- O'Brien S. J., Macintyre R. J. The -glycerophosphate in Drosophila melanogaster. II. Genetic aspects. Genetics. 1972 May;71(1):127–138. doi: 10.1093/genetics/71.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richmond R. C., Gilbert D. G., Sheehan K. B., Gromko M. H., Butterworth F. M. Esterase 6 and reproduction in Drosophila melanogaster. Science. 1980 Mar 28;207(4438):1483–1485. doi: 10.1126/science.6767273. [DOI] [PubMed] [Google Scholar]