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. 1983 Oct;80(20):6413–6417. doi: 10.1073/pnas.80.20.6413

Comparative localization of acetylcholinesterase and pseudocholinesterase during morphogenesis of the chicken brain.

P G Layer
PMCID: PMC394308  PMID: 6578516

Abstract

The histochemical localization of specific acetylcholinesterase (AcChoEase) and nonspecific cholinesterase (BtChoEase) is described during the early morphogenesis of the whole chicken head with main emphasis on the visual system. It is found that: (i) Expression of AcChoEase is an early differentiation event in the entire brain. Its pattern of first appearance on the external part of the neuroepithelium correlates with the general spatio-temporal pattern of differentiation. AcChoEase thus represents an early differentiation marker. (ii) The late pattern of AcChoEase (at E18), reflecting at least partially the distribution of synaptic AcChoEase shows no direct correlation to the distributions found at early stages when synapses are not yet formed. This argues for a nonsynaptic function of the early appearing AcChoEase. (iii) BtChoEase in early nervous tissue is diffusely localized on the ventricular side of the neural tube. At later stages it becomes concentrated on the ependymal layer as well as along fibers reaching from this inner layer outwards. Minor activities appear in specific external layers of tectum and retina. (iv) During the course of differentiation the enzymes express pronounced graded distributions within the areas where they are detectable. (v) Mesenchymal and epidermal BtChoEase is abundant in the entire head. Prominent amounts of activity are expressed on the rostral epidermis, along the eye cup next to the sclera, and on the rostro-dorsal surrounding of the optic nerves. The results are discussed in the light of possible morphogenetic functions of these enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fitzpatrick-McElligott S., Stent G. S. Appearance and localization of acetylcholinesterase in embryos of the leech Helobdella triserialis. J Neurosci. 1981 Aug;1(8):901–907. doi: 10.1523/JNEUROSCI.01-08-00901.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Graybiel A. M., Ragsdale C. W., Jr Pseudocholinesterase staining in the primary visual pathway of the macaque monkey. Nature. 1982 Sep 30;299(5882):439–442. doi: 10.1038/299439a0. [DOI] [PubMed] [Google Scholar]
  3. Henke H., Fonnum F. Topographical and subcellular distribution of choline acetyltransferase and glutamate decarboxylase in pigeon optic tectum. J Neurochem. 1976 Aug;27(2):387–391. doi: 10.1111/j.1471-4159.1976.tb12258.x. [DOI] [PubMed] [Google Scholar]
  4. KARNOVSKY M. J., ROOTS L. A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES. J Histochem Cytochem. 1964 Mar;12:219–221. doi: 10.1177/12.3.219. [DOI] [PubMed] [Google Scholar]
  5. Kusano K., Miledi R., Stinnakre J. Acetylcholine receptors in the oocyte membrane. Nature. 1977 Dec 22;270(5639):739–741. doi: 10.1038/270739a0. [DOI] [PubMed] [Google Scholar]
  6. LaVail J. H., Cowan W. M. The development of the chick optic tectum. II. Autoradiographic studies. Brain Res. 1971 May 21;28(3):421–441. [PubMed] [Google Scholar]
  7. Layer P. G., Kotz S. Asymmetrical developmental pattern of uptake of Lucifer Yellow into amacrine cells in the embryonic chick retina. Neuroscience. 1983 Aug;9(4):931–941. doi: 10.1016/0306-4522(83)90282-8. [DOI] [PubMed] [Google Scholar]
  8. Liu L., Layer P. G., Gierer A. Binding of FITC-coupled peanut-agglutinin (FITC-PNA) to embryonic chicken retinas reveals developmental spatio-temporal patterns. Brain Res. 1983 Jun;284(2-3):223–229. doi: 10.1016/0165-3806(83)90007-x. [DOI] [PubMed] [Google Scholar]
  9. Meedel T. H., Whittaker J. R. Development of acetylchilinesterase during embryogenesis of the ascidian Ciona intestinalis. J Exp Zool. 1979 Oct;210(1):1–10. doi: 10.1002/jez.1402100102. [DOI] [PubMed] [Google Scholar]
  10. Miki A., Mizoguti H. Proliferating ability, morphological development and acetylcholinesterase activity of the neural tube cells in early chick embryos. An electron microscopic study. Histochemistry. 1982;76(3):303–314. doi: 10.1007/BF00543953. [DOI] [PubMed] [Google Scholar]
  11. Nichols C. W., Koelle G. B. Comparison of the localization of acetylcholinesterase and non-specific cholinesterase activities in mammalian and avian retinas. J Comp Neurol. 1968 May;133(1):1–16. doi: 10.1002/cne.901330102. [DOI] [PubMed] [Google Scholar]
  12. RENNELS E. G. An experimental study of cytoplasmic inclusions in adrenal cortical cells of the immature rat. Anat Rec. 1952 Mar;112(3):509–527. doi: 10.1002/ar.1091120304. [DOI] [PubMed] [Google Scholar]
  13. SHEN S. C., GREENFIELD P., BOELL E. J. Localization of acetylcholinesterase in chick retina during histogenesis. J Comp Neurol. 1956 Dec;106(2):433–461. doi: 10.1002/cne.901060211. [DOI] [PubMed] [Google Scholar]
  14. Strudel G., Gateau G. Etude de l'action têratogène du sulfate de nicotine sur les stades jeunes de l'embryon de poulet. C R Acad Sci Hebd Seances Acad Sci D. 1971 May 10;272(19):2480–2483. [PubMed] [Google Scholar]
  15. Strudel G., Gateau G. Sur l'existence de récepteurs cholinergiques chez le très jeune embryon de poulet. C R Acad Sci Hebd Seances Acad Sci D. 1977 Feb 7;284(6):469–472. [PubMed] [Google Scholar]
  16. Villani L., Contestabile A. Cytochemical study of cholinesterases in the normal and retino-derived optic tectum of reptiles. J Hirnforsch. 1982;23(1):55–66. [PubMed] [Google Scholar]

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