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. 2014 Mar 6;4:33. doi: 10.3389/fcimb.2014.00033

Specific sulfation and glycosylation—a structural combination for the anticoagulation of marine carbohydrates

Vitor H Pomin 1,*, Paulo A S Mourão 1,*
PMCID: PMC3944403  PMID: 24639954

Abstract

Based on considered achievements of the last 25 years, specific combinations of sulfation patterns and glycosylation types have been proved to be key structural players for the anticoagulant activity of certain marine glycans. These conclusions were obtained from comparative and systematic analyses on the structure-anticoagulation relationships of chemically well-defined sulfated polysaccharides of marine invertebrates and red algae. These sulfated polysaccharides are known as sulfated fucans (SFs), sulfated galactans (SGs) and glycosaminoglycans (GAGs). The structural combinations necessary for the anticoagulant activities are the 2-sulfation in α-L-SGs, the 2,4-di-sulfation in α-L-fucopyranosyl units found as composing units of certain sea-urchin and sea-cucumber linear SFs, or as branching units of the fucosylated chondroitin sulfate, a unique GAG from sea-cucumbers. Another unique GAG type from marine organisms is the dermatan sulfate isolated from ascidians. The high levels of 4-sulfation at the galactosamine units combined with certain levels of 2-sulfation at the iduronic acid units is the anticoagulant structural requirements of these GAGs. When the backbones of red algal SGs are homogeneous, the anticoagulation is proportionally dependent of their sulfation content. Finally, 4-sulfation was observed to be the structural motif required to enhance the inhibition of thrombin via heparin cofactor-II by invertebrate SFs.

Keywords: algae, carbohydrate-based drug development, fucosylated chondroitin sulfate, sea cucumber, sea urchin, sulfated galactan, sulfated fucan

Introduction

Marine organisms represent a very special source of potential therapeutic molecules with unique structures. Among innumerous of these compounds, the sulfated polysaccharides (SPs) have awakened great interest in the scientific community. This happens mostly because of the fact that these compounds hold the characteristic of being naturally polyanionic. This feature makes the SPs suitable to interact with important functional proteins, especially those involved in the balance of health and disease. The nature and quality of the SP-protein interactions control and regulate the activity of these functional proteins in the body. Although the affinity of these molecular complexes are mostly driven by electrostatic interactions in which sulfation content of the SPs play a crucial role, the overall structural features of the SPs are still more influential in the process (Pomin, 2009). The structural features of the SPs involved into the quality of these molecular interactions are sulfation patterns and glycosylation. This latter includes anomeric and enantiomeric configurations, glycosidic linkage position, monosaccharide type and composition.

In the last 25 years, our group has made great efforts in scientific researches related with marine and medicinal glycobiology. As a consequence, many sulfated fucans (SFs), sulfated galactans (SGs) and glycosaminoglycans (GAGs) of new structures have been characterized and described (Vieira and Mourão, 1988; Mourão, 2004; Pomin and Mourão, 2008). We believe that we have fully characterized over 20 new structures of these marine sulfated polysaccharides (MSPs) in our studies (Vieira and Mourão, 1988; Mourão, 2004; Pomin and Mourão, 2008; Pomin, 2009, 2012a,b, 2014a,b). We have also submitted most of these new MSPs to in vitro experiments to assess their possible anticoagulant effects (Mourão, 2004; Pomin and Mourão, 2008; Pomin, 2009). Curiously, we noticed that even though bearing significant levels of sulfation, some of these MSPs have insignificant effects toward the coagulation system; while other MSPs, even carrying lower sulfation content, can show surprising levels of anticoagulant activity (Mourão, 2004; Pomin, 2009). Moreover, some SFs and SGs, even though exhibiting nearly equal sulfation levels, but within different sulfation patterns, have completely different anticoagulant effects. This observation has clearly proved the concept that sulfation and thus electronegative-charge density in marine carbohydrates are not the solely structural determinants for the resultant anticoagulant activities of these molecules.

Since we have characterized and used many different structures in our anticoagulant tests across the last years, we should be able to state by now some of the structural features of the MSPs necessary for their differential anticoagulant properties. Based on comparative and systematic analyses on the structure-anticoagulation relationships of certain MSPs, we noticed that to achieve a good anticoagulant response, certain structural combinations of sulfation and glycosylation are indeed required. In these analyses, we have particularly given more preference to examine SFs, SGs and GAGs from marine invertebrates or red algae, since these organisms can provide molecules of well-defined chemical structures (Mourão, 2004; Pomin, 2009, 2012c; Pomin and Mourão, 2012). This kind of structural regularity has facilitated interpretation and this in turn enables us to establish advanced structure-anticoagulation relationships (Pereira et al., 2002; Pomin, 2009, 2012c). Here, we describe based on data of some of our previous works, the major structural combinations of the invertebrate and red algal MSPs that have been proved to be necessary to make a satisfactory anticoagulant effect.

The role of SFs and SGs of well-defined structures: algal vs. invertebrate molecules

SFs are a class of SPs composed mostly of α-L-fucopyranosyl (Fucp) units. SFs can be extracted mostly from marine organisms, such as sea-urchins (echinoidea), sea-cucumbers (holothuroidea) and brown algae (phaeophyta). These molecules are also known as fucoidans when isolated from brown algae. Besides Fucp, the fucoidans are also composed of other sugars, such as xylose and uronic acids. This heterogeneous monosaccharide composition, associated with the lack or an unclear structural pattern of regularity and the presence of branching residues, makes the establishment of the structure-function relationships for algal molecules very hard (Pereira et al., 1999). On the other hand, the SFs isolated from invertebrates can reveal structures quite regular (Figures 1A–I). This type of structural pattern helps to achieve advanced structure-function correlations about their anticoagulant properties. Using the invertebrate SFs, we are able to understand which structural features are important for the anticoagulant activity of these molecules (Pereira et al., 1999; Pomin, 2009, 2012b,c). Below, some of these influential structural features on coagulation of the MSPs of well-defined chemical structures will be described.

Figure 1.

Figure 1

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Structural representation of the repetitive oligomeric units of the SFs (A–I) and SGs (J–O, inside the box) of well-defined chemical structures isolated from cell wall of sea cucumbers (A), from egg jelly coat of sea urchins (B–K), from tunic of ascidians (L,M), and from cell wall of red algae (N,O). Modified with permission from Pomin (2012b). These MSP are composed of α-L-fucopyranoses (α-l-Fucp) (A–I), α-L-galactopyranoses (α-L-Galp) (I, M-O), or β-D-galactopyranosyl units (β-D-Galp) (K,N,O). The species-specific structures vary in sulfation patterns (exclusively 2-O- and/or 4-O-, or 3-O-positions), in glycosidic linkages: α(1→3) (A–C,E,F,H–K), α(1→4) (D,G,L,M), β (1→3) (K), or alternating β (1→4) and α(1→3) (N,O); and in number of composing residues of the repetitive units: tetrasaccharides (A–D), trisaccharides (F), disaccharides (K,N,O), or monosaccharides (E,G–J,L), all in linear chains, except (M). The sulfation groups are highlighted by red dashed ellipses or rectangle when just percentage can be estimated. The structures are the following: (A) Ludwgothurea grisea [→3)-α-L-Fucp-2,4(OSO3−)-(1→3)-α-L-Fucp-(1→3)-α-L-Fucp-2(OSO3−)-(1→3)-α-L-Fucp-2(OSO3−)-(1→]n (Mulloy et al., 1994); (B) Lytechinus variegates I [→3)-α-L-Fucp-2,4(OSO3−)-(1→3)-α-L-Fucp-2(OSO3−)-(1→3)-α-L-Fucp-2(OSO3−)-(1→3)-α-L-Fucp-4(OSO3−)-(1→]n (Mulloy et al., 1994); (C) Strongylocentrotus pallidus [→3)-α-L-Fucp-4(OSO3−)-(1→3)-α-L-Fucp-4(OSO3−)-(1→3)-α-L-Fucp-2(OSO3−)-(1→3)-α-L-Fucp-2(OSO3−)-(1→]n (Vilela-Silva et al., 2002); (D) Arbacia lixula [→4)-α-L-Fucp-2(OSO3−)-(1→4)-α-L-Fucp-2(OSO3−)-(1→4)-α-L-Fucp-(1→4)-α-L-Fucp-(1→]n (Alves et al., 1997); (E) Strongylocentrotus purpuratus I ~80% [→3)-α-L-Fucp-2,4(OSO3−)-(1→]n and ~20% [→3)-α-L-Fucp-2(OSO3−)-(1→]n (Alves, Mulloy, Moy, Vacquier and Mourão, 1998) and (F) S. purpuratus II [→3)-α-L-Fucp-2,4(OSO3−)-(1→3)-α-L-Fucp-4(OSO3−)-(1→3)-α-L-Fucp-4(OSO3−)-(1→]n (Alves, Mulloy, Moy, Vacquier and Mourão, 1998);(G) Strongylocentrotus droebachiensis [→4)-α-L-Fucp-2(OSO3−)-(1→]n (Vilela-Silva et al., 2002); (H) Strongylocentrotus franciscanus [3)-α-L-Fucp-2(OSO3−)-(1→]n (Vilela-Silva et al., 1999); (I) L. varieagtus II [3)-α-L-Fucp-4(OSO3−)-(1→]n (Cinelli et al., 2007); (J) Echinometra lucunter [→3-α-L-Galp-2(OSO3−)-1→]n (Alves et al., 1997); (K) Glyptocidaris crenularis [→3-β-L-Galp-2(OSO3−)-1→3-β-L-Galp-1→]n (Castro et al., 2009); (L) Herdmania monus [→4)-α-L-Galp-3(SO3−)-(1→]n (Santos et al., 1992); (M) Styela plicata {→4)-α-L-Galp-2[→1)- α-L-Galp-3(OSO3−)]-3(OSO3−)-(1→}n (Mourão and Perlin, 1987); (N,O) both Botriocladia occidentalis and Gelidium crinale express structures of [3-β-D-Galp-1→4-α-Gal-1→]n with different sulfation contents (Farias et al., 2000; Pereira et al., 2005b).

SGs are a class of SPs composed of α-L-, α-D-, or β-D-galactopyranosyl (Galp) units. SGs can be found in green algae (clorophyta), red algae (rodophyta), sea-urchins and ascidians which are also known as tunicates (ascidiacea). Like SFs, algal SGs have structures more complex than the regular and simpler invertebrate counterparts. In green algae, for example, the sulfation pattern is usually complex, with additional substitutions like pyruvates, and the possibility of branches (Farias et al., 2008). In red algae, although the SGs show heterogeneous sulfation patterns, they are usually composed of disaccharide repeating units of 3-linked β-D-Galp and 4-linked α-Gal units in their backbones. Sometimes, the latter unit can be seen forming an extra carbon ring which results in an anhydro-sugar (Quinderé et al., 2014). This is an additional heterogeneity that enhances structural complexity in the red algal molecule. However, some red algal species can show very simple structures whose sulfation patterns vary accordingly to the species of extraction. Two examples of these structures are shown at panels N and O of Figure 1. Advanced structure-function correlations can be reached when these red algal SGs are used, as opposed to the more heterogeneous SGs from red or green algae. Conversely, the invertebrate SGs are very often composed of well-defined chemical structures (Figures 1J–M), which allow accurate structure-function correlations.

The information in the two previous paragraphs have made clear the advantages of the structures of the invertebrate SFs and SGs over the algal molecules, except few cases of red algal molecules (Figures 1N,O). These advantages come from the fact that the invertebrate molecules and the regular red algal SGs (Figure 1), which are composed of well-defined chemical structures, can be successfully used in structure-function relationship studies (Table 1). These studies allow prediction of the most influential structural combinations of the MSPs to achieve desirable anticoagulant responses. This knowledge is relevant to the development of these molecules as future therapeutic candidates.

Table 1.

Anticoagulant activities of MSPs of well-defined structures (Figure 1) measured by aPTTa and by IC50 for thrombin citation(IIa) and factor Xa inhibition in the presence of antithrombin citation(AT) or heparin cofactor II (HCII).

Polysaccharide Source Structure (Figure) APTTcitation(IU/mg) a IC50 citation(μg/mL)
IIa/AT IIa/HCII Xa/AT
3-linked sulfated α-L-fucans S. purpuratus I 1E 76 0.3 0.3 2
S. purpuratus II 1F 10 0.9 2 Ndb
S. pallidus 1C 18 >500 >500 >500
L. variegatus I 1B 3 >500 >500 >500
L. variegatus II 1I Nd Nd Nd Nd
S. franciscanus 1H ~2 >500 >500 250
L. grisea 1A <1 >500 >500 >500
4-linked sulfated α-L-fucans S. droebachiensis 1G <1 Nd Nd Nd
A. lixula 1D ~2 150 150 >500
sulfated α-L-galactans E. lucunter 1J 20 3 6 20
G. crenularis 1K Nd Nd Nd Nd
H. monus 1L ~2 >500 >500 >500
S. plicata 1M <1 >500 >500 >500
algal SGs B. occidentalis 1N 93 0.02 1.1 2.5
G. crinale 1O 65 0.02 25 1.5
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Modified with permission from Pomin (2009).

a

The activity is expressed as international units/mg using a parallel standard curve based on the International Heparin Standard citation(193 units/mg).

b

Not determined.

Marine GAGs have unique structures

Marine GAGs have different structures than those present in common mammal GAGs. For example, dermatan sulfate (DS) isolated from the ascidian species Styela plicata is composed of [→4)-α-L-IdoA-(2R1,3R2)-(1→3)-β-D-GalNAc-(4R3,6R4)-(1→]n, where IdoA is iduronic acid, GalNAc is N-acetyl galactosamine. The R1, R2, R3, and R4 are sulfate groups at 66, < 5, 94, and 6 percent, respectively (Pavão et al., 1998). The S. plicata is mostly 2-sulfated at the IdoA unit but largely 4-sulfated at the GalNAc unit. Conversely, the commonest mammalian DS is mostly composed of 2-sulfated IdoA units together with occasional C4 sulfation at GalNAc units. Another different GAG from marine invertebrates is the fucosylated chondroitin sulfate (FucCS) isolated from sea-cucumbers. These molecules are composed of the following structure {→3)-β-D-GalNAc-(1→4)-[α-L-Fucp-(1→3)]-β-D-GlcA-(1→}n (Figure 2) (Pomin, 2014a). The branching Fucp unit can be sulfated at the 2, and/or 3 and/or 4-positions within different percentages according to the species of occurrence (Table 2). Conversely, the commonest chondroitin sulfates (CSs) of mammals are made of the following structure [→4)-β-D-GlcA-(1→3)-β-D-GalNAc-(1→]n, in which the GalNAc units can be either mostly 4-sulfated (CS-A) or predominantly 6-sulfated (CS-C) (Pomin et al., 2012).

Figure 2.

Figure 2

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Structural representation of the holothurian FucCS. The monosaccharides are indicated by rectangles. They are α-l-fucopyranose (l-Fucp), β-d-glucuronic acid (d-GlcA), and N-acetyl β-d-galactosamine (d-GalNAc). The glycosidic linkage types are indicated in ellipses. Modified with permission from Pomin (2014a).

Table 2.

Sulfation patterns (proportions of the branching sulfated fucose units) and the anticoagulant potential (measured by aPTT) of FucCS from 12 sea cucumber species analyzed so far.

Species Fuc0S Fuc3S Fuc4S Fuc2S4S Fuc3S4S aPTT References
Ludwigothurea griseaa 0 - ~49 ~20 ~17 55b Mourão et al., 1996; Fonseca et al., 2009
Pearsonothuria graeffei - - 81.6 18.4 - 35c Chen et al., 2011
Holothuria vagabunda 25.6 - 50.2 15.8 8.4 42c Chen et al., 2011
Stichopus tremulus - - 24.8 22.4 52.8 135c Chen et al., 2011
Isostichopus badionotus - - 4.1 95.9 - 183c Chen et al., 2011
Thelenata ananas 0 ~25 ~22 ~53 0 348d Wu et al., 2010, 2012
Stichopus japonicuse 0 Ndf 11.1 55.6 33.3 Nsg Yoshida and Minami, 1992
Holothuria edulish - - Nd 18 Nd 89i Luo et al., 2013
Apostichopus japonicash - - Nd 45 Nd 116i Luo et al., 2013
Holothuria nobilisj - Nd Nd - Nd 59i Luo et al., 2013
Acaudina molpadioideak - - - - - Ncl Ye et al., 2012
Athyonidium chilensisk - - - - - Ncl Matsuhiro et al., 2012
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Reprint with permission from Pomin (2014a).

a

The CS backbone of FucCS from L. grisea has been extensively characterized. It is composed of GalNAc units with the following substitution percentages: 12% 4,6-di-sulfated, 53% 6-mono-sulfated, 4% 4-mono-sulfated, and 31% non-sulfated (Fonseca et al., 2009).

b

aPTT values expressed as international units/mg citation(IU/mg) using a parallel standard curve based on the International Heparin Standard (UFH) whose activity is 229 units/mg (Fonseca et al., 2009).

c

aPTT values expressed as international units/mg citation(IU/mg) using a parallel standard curve based on the International Heparin Standard citation(UFH) whose activity is 150 units/mg (Chen et al., 2011).

d

aPTT values expressed as international units/mg citation(IU/mg) using a parallel standard curve based on the International Heparin Standard citation(UFH) whose activity is 204 units/mg (Wu et al., 2012).

e

The CS backbone of this FucCS was mostly characterized as CS-E (Nagase et al., 1995), which is predominantly composed of 4,6-O-di-sulfated GalNAc units.

f

Not determined.

g

Not studied.

h

Although the mono-4S and di-3S4S fucosyl units have been assigned in the FucCS of H. edulis and A. japonicas in Luo et al. (2013), the amounts of these units were not provided therein.

i

aPTT values expressed as international units/mg citation(IU/mg) using a parallel standard curve based on the International Heparin Standard citation(UFH) whose activity is 212 units/mg (Luo et al., 2013).

j

The FucCS from H. nobilis was studied by NMR but the anomeric signals belonging to the fucose residues were rather to weak and broad to allow integration and further quantitation of their proportions. However, mono-3S, mono-4S, and di-3S4S fucosyl units were clearly observed (Luo et al., 2013).

k

Structures studied by Fourier transformed-infrared spectroscopy. Just a few structural features were raised. The sulfation patterns of FucCS from these two holothurian species are still an unknown.

l

Not clear. Although the aPTT assay was undertaken and values were measured for different FucCSs concentration, the final values in IU/mg in comparison with a standard UFH curve were not provided.

Anticoagulant mechanisms of action of the MSPs

The effects of MSPs on hemostasis are the mostly studied medical activities of these compounds. The mechanisms of action in this particular clinical activity reside basically on the inhibition of some coagulation proteases like thrombin citation(IIa) and factor Xa, via their natural inhibitors, named serpins citation(serine-protease inhibitors). The most common serpins of this system are antithrombin citation(AT) and heparin cofactor II citation(HCII). Although at different degrees of response, the majority of the MSPs described in this review, the ascidian DS (Pavão et al., 1998; Vicente et al., 2004; Kozlowski et al., 2011), the sea-cucumber FucCS (Mourão et al., 1996; Mourão, 2004), the algal SFs and SGs (Pereira et al., 1999; Farias et al., 2000; Mourão, 2004) and the invertebrate SFs and SGs (Pereira et al., 1999, 2002; Pomin, 2012c) have either negligible or impressive effects in this serpin-dependent mechanism. The resultant anticoagulant activities of these MSPs (Tables 1, 2) are intimately and ultimately dependent on their structural features, as described below.

Besides the common serpin-dependent anticoagulant mechanism, the FucCS from the sea-cucumber Ludwigothurea grisea, which its fucosyl unit is mostly 2,4-di-sulfated (Figure 2; Table 2), and the SG from the red alga Botryocladia occidentalis (Figure 1N) have also shown serpin-independent anticoagulant mechanism (Glauser et al., 2008, 2009). Initially, their anticoagulant actions were primarily attributed by their capacity in potentiate the inhibition of factors Xa and IIa, via either AT or HCII. However, the sea-cucumber FucCS and the red algal SG are also known to inhibit the generation of factor Xa and IIa by interfering in the formation of the blood cofactor complexes at the surface of the cells. Factor Xa is activated mainly by the intrinsic tenase complex, while IIa is converted from II by the prothrombinase complex. FucCS and SG were shown the ability to inhibit the activation of these tenase and prothrombinase complexes (Glauser et al., 2008, 2009). The formation of these complexes is a key step for the generation and amplification of the coagulation cofactors. This serpin-independent mechanism has also been reported for other types of SGs (Quinderé et al., 2014). Unfortunately, advanced structure-function correlations are yet to be established for this novel mechanism. Therefore, the structural requirements necessary to achieve the anticoagulant activity via the serpin-independent mechanism are still unknown. For this reason, we will keep this new anticoagulant mechanism out of discussion here.

The anticoagulant structural combinations

The anticoagulant structural features responsible for the anticoagulant activity of the MSPs can be determined from systematic analyses using the MSPs of well-defined chemical structures (Figure 1). On this way, comparing all the structures in Figure 1, one can discern structural similarities and differences in these molecules. For example, both the SF from Strongylocentrotus franciscanus (Figure 1H), and the SG from Echinometra lucunter (Figure 1J) present the same sulfation pattern (exclusive and entirely 2-sulfated), the same anomeric configuration (α-form), the same glycosidic linkage (1→3), and based on previous works (Pereira et al., 2005a), the same molecular mass (~100 kDa). Their single difference is the sugar type (Fucp or Galp). Interestingly, this single structural difference is itself enough to promote great changes in the anticoagulant outcomes of these homopolysaccharides. The 2-sulfated α-galactan from E. lucunter exhibits a significant anticoagulant activity monitored by the activated partial thromboplastin time (aPTT) method (Pomin, 2014b). The anticoagulant potential of this SG was determined to be 20 IU mg, although almost 10-fold less than unfractionated heparin (UFH) (Table 1). The specific anticoagulant assay with the purified proteases revealed that this SG enhances both IIa and factor Xa inhibition by either AT or HCII (Table 1). Conversely, the anticoagulant effect of 2-sulfated α-fucan from S. franciscanus is exclusively based on catalysis of AT inhibition over factor Xa, although it is 12.5-fold less active than the α-SG. This single effect on the Xa/AT system explains the much lower activity of the compound from S. franciscanus (aPTT of ~2 IU mg−1, 100-fold less active than UFH) since the anti-Xa activity has a relatively minor influence on the aPTT. This is an illustrative and typical example of a sugar-type-dependent biological effect of polysaccharides. The structural combination of 2-sulfation with 3-linked α-Galp units results therefore in the anticoagulant response.

Based on this same systematic and comparative analysis, the SGs from the red algae Botryocladia occidentalis and Gelidium crinale were seen to exhibit identical backbones, and the same chain sizes (Farias et al., 2000; Pereira et al., 2005b). However, there are slight differences in their sulfation patterns (Figures 1M,O). As summarized in Table 1, these structural differences account for the 30% difference in anticoagulant activity as observed by the aPTT values of these algal macromolecules and the even greater difference in catalytic effect of the SP from B. occidentalis on the HCII-mediated anti-IIA activity, which was approximately 25-fold more than the catalytic effect of the SP from G. crinale. When the backbones of the red algal SGs are identical but bearing sulfation as their main difference, the anticoagulant activity seems to be proportionally dependent of the sulfation content (Table 1).

The structural requirements for the interaction of the MSP of well-defined chemical structures with the coagulation cofactors and their target proteases and inhibitors were determined to be very stereospecific (Mourão, 2004; Pomin and Mourão, 2008; Pomin, 2009). The site of sulfation has a major impact on the activity. This can be illustrated by the fact that 2,4-di-sulfated units have an amplifying effect on the AT-mediated anticoagulant activity in the series of 3-linked α-L-fucans (Figure 1; Table 1). Specific sulfation sites are required for the interaction with plasma serine-protease inhibitors. Note the occurrence of the 4-sulfated unit content in the 3-linked α-L-fucans vs. their anticoagulant activities (Table 1). L. variegatus (a single 4-sulfated unit/tetrasaccharide, Figure 1B, has 3 IU/mg of activity), S. pallidus (a double 4-sulfated unit/tetrasaccharide, Figure 1C, has 18 IU/mg of activity), and S. purpuratus isotype II (a double 4-sulfated unit/trisaccharide, Figure 1F, has 76 IU/mg of activity). This 4-sulfation is the structural motif required to enhance the inhibition of IIa by HCII. In contrast, the presence of 2-sulfated residues seems to have a deleterious effect on HCII-mediated anti-IIA activity of the polysaccharides (Mourão, 2004).

In the studies using the ascidian DS, Pavão and co-authors demonstrated that 4-sulfation at GalNAc units together with some 2-sulfation at the IdoA units is a structural motif of anticoagulant properties in these molecules (Pavão et al., 1998). This was observed based on the fact that DS from the species Styela plicata and Halocynthia pyriformis have large amounts of 4-O-sulfated GalNac units (94 and 99%, respectively), together with the reasonable amounts of 66 and 70% of 2-sulfation at the IdoA units. Their anticoagulant actions were measured by aPTT and shown to be 11 and 8 IU/mg, respectively; while the activity of the DS from bovine mucosa was shown to be only 2 units/mg (Pavão et al., 1998). Unlike the ascidian DSs, the mammalian DS is largely 2-sulfated at the IdoA unit and much less sulfated at the 4-position of the GalNAc units, as above-mentioned.

The sulfated fucosyl branches in the sea-cucumber FucCS were shown to be essential to their anticoagulant activities. This statement is based on the fact that when these branching units are removed, for example, by mild acid hydrolysis, or desulfated, by desulfation reactions, their anticoagulant effects disappears (Pomin, 2014a). Besides the necessary existence of these branching units, their sulfation patterns are also influential to the levels of anticoagulant activity. This can be seen from the structures and aPTT values of Table 2. The 2,4-di-sulfation in the fucosyl branching units appears to be the best sulfation pattern to the anticoagulant activity of this class of SPs. The importance of the 2,4-di-sulfation in Fucp units was already pointed out in the work of Fonseca et al. (2009), as above for the AT-mediated anticoagulant activities of the series of 3-linked α-L-fucans (Figure 1, Table 1). In the work of Fonseca et al. (2009), the 2,4-di-sulfated Fucp units were reported to be crucial to the anticoagulant activity of both sea-cucumber FucCS molecules and the linear SFs from invertebrates (Figure 1, Table 1).

Major conclusions

Here, we have made clear the relevance of certain structural combinations of sulfation and glycosylation to the anticoagulant activity of the marine carbohydrates of well-defined chemical structures. For example, 2-sulfation together with 3-linked α-L-Galp units, as found in the SG from the sea-urchin E. lucunter (Figure 1J), represents one of these structural combinations. Another combination is the 2,4-di-sulfation with α-L-Fucp units. This combination can be found either in linear SFs of sea-urchins and sea-cucumbers (Figures 1A,B,F), or in the branched FucCS molecules (Figure 2, Table 2). The FucCS is a unique GAG found exclusively in sea-cucumbers. Another unique GAG from marine sources are the ascidian DS. For a good anticoagulant response of these GAGs, the 4-sulfation in GalNAc units combined with occasional 2-sulfation in IdoA units seems to be essential. When SGs from red algae exhibit simple backbones in which sulfation comprises the single modification and difference, sulfation content regardless the pattern seems to be relevant for the anticoagulant activity. In this case, the anticoagulant activity increases proportionally with the sulfation content. Finally, in invertebrate SFs, the 4-sulfation was observed to be a structural motif required to enhance the HCII-mediated inhibition of IIa. All these conclusions are summarized at Table 3 for a straightforward representation. Although we have clearly revised the relevance of sulfation and glycosylation to the anticoagulant activity of MSPs, molecular weights are also known to be influential to the biological activity (Melo et al., 2004; Quinderé et al., 2014). To ensure that our interpretation on the anticoagulant effects of MSPs have been based solely on sulfation and glycosylation patterns, our comparative analyses using the different MSP structures were performed on samples of similar molecular weights. This procedure disregards the contribution from differential molecular weights.

Table 3.

Summary of the structural requirements and effects in anticoagulation of the MSPs.

Structural requirement Outcome
2-sulfated 3-linked α-L-Galpa Enhance serpin (HCII and AT) inhibitory activity over the coagulation proteases (IIa and Xa)
2,4-disulfation in Fucpb
4-sulfated GalNAc + 2-sulfated IdoA in ascidian DSc
Sulfation content in red algal homogeneous SGsd
4-sulfation in invertebrate 3-linked SFse Enhance HCII-dependent IIa inhibition
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a

(Pereira et al., 2005a).

b

(Fonseca et al., 2009).

c

(Pavão et al., 1998).

d

(Pereira et al., 2005b).

e

(Pereira et al., 2002).

One of the major goals of research programs involved with drug development nowadays, especially those related with potential carbohydrate-based drug candidates, is to understand the structural requirements of the new compounds in their specific therapeutic functions. Here, we have presented in a clear and straightforward way some of these structural requirements of the invertebrate and red algal SPs for their anticoagulant properties.

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors acknowledge Prof. Eliana Barreto-Bergter for the kind invitation to contribute a paper to the Research Topic “Glycan diversity in fungi, bacteria and sea organisms” of Frontiers in Cellular and Infection Microbiology. Vitor H. Pomin and Paulo A. S. Mourão are also equally grateful to the Brazilian financial agencies FAPERJ and CNPq for their financial supports. The authors state that the content of this work is solely the responsibility of them and does not necessarily represent the official views of the funding agencies.

Glossary

Abbreviations

aPTT

activated partial thromboplastin time

AT

antithrombin

DS

dermatan sulfate

Fucp

L-fucopyranose

FucCS

fucosylated chondroitin sulfate

GAGs

glycosaminoglycans

GalNAc

N-acetyl D-galactosamine

Galp

galactopyranose

GlcA

D-glucuronic acid

HCII

heparin cofactor II

IdoA

L-iduronic acid

MSPs

marine sulfated polysaccharides

SGs

sulfated galactans

SFs

sulfated fucans

SPs

sulfated polysaccharides

UFH

unfractionated heparin

IIa

thrombin

XIIa

factor XII activated.

References

  1. Alves A.-P., Mulloy B., Diniz J. A., Mourão P. A. (1997). Sulfated polysaccharides from the egg jelly layer are species-specific inducers of acrosomal reaction in sperms of sea urchins. J. Biol. Chem. 272, 6965–6971 10.1074/jbc.272.11.6965 [DOI] [PubMed] [Google Scholar]
  2. Alves A.-P., Mulloy B., Moy G. W., Vacquier V. D., Mourão P. A. (1998). Females of the sea urchin Strongylocentrotus purpuratus differ in the structures of their egg jelly sulfated fucans. Glycobiology 8, 939–946 10.1093/glycob/8.9.939 [DOI] [PubMed] [Google Scholar]
  3. Castro M. O., Pomin V. H., Santos L. L., Vilela-Silva A.-C., Hirohashi N., Pol-Fachin L., et al. (2009). A unique 2-sulfated {beta}-galactan from the egg jelly of the sea urchin Glyptocidaris crenularis: conformation flexibility versus induction of the sperm acrosome reaction. J. Biol. Chem. 284, 18790–18800 10.1074/jbc.M109.005702 [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen S., Xue C., Yin L., Tang Q., Yu G., Chai W. (2011). Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers. Carbohydr. Polym. 83, 688–696 10.1016/j.carbpol.2010.08.04023313164 [DOI] [Google Scholar]
  5. Cinelli L. P., Castro M. O., Santos L. L., Garcia C. R., Vilela-Silva A.-C., Mourão P. A. (2007). Expression of two different sulfated fucans by females of Lytechinus variegatus may regulate the seasonal variation in the fertilization of the sea urchin. Glycobiology 17, 877–885 10.1093/glycob/cwm058 [DOI] [PubMed] [Google Scholar]
  6. Farias E. H., Pomin V. H., Valente A. P., Nader H. B., Rocha H. A., Mourão P. A. (2008). A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum. Glycobiology 18, 250–259 10.1093/glycob/cwm139 [DOI] [PubMed] [Google Scholar]
  7. Farias W. R., Valente A. P., Pereira M. S., Mourão P. A. (2000). Structure and anticoagulant activity of sulfated galactans. Isolation of a unique sulfated galactan from the red algae Botryocladia occidentalis and comparison of its anticoagulant action with that of sulfated galactans from invertebrates. J. Biol. Chem. 275, 29299–29307 10.1074/jbc.M002422200 [DOI] [PubMed] [Google Scholar]
  8. Fonseca R. J., Santos G. R., Mourão P. A. (2009). Effects of polysaccharides enriched in 2,4-disulfated fucose units on coagulation, thrombosis and bleeding. Practical and conceptual implications. Thromb. Haemost. 102, 829–836 10.1160/TH08-11-0773 [DOI] [PubMed] [Google Scholar]
  9. Glauser B. F., Pereira M. S., Monteiro R. Q., Mourão P. A. (2008). Serpin-independent anticoagulant activity of a fucosylated chondroitin sulfate. Thromb Haemost. 100, 420–428 10.1160/TH08-04-0210 [DOI] [PubMed] [Google Scholar]
  10. Glauser B. F., Rezende R. M., Melo F. R., Pereira M. S., Francischetti I. M., Monteiro R. Q., et al. (2009). Anticoagulant activity of a sulfated galactan: serpin-independent effect and specific interaction with factor Xa. Thromb Haemost. 102, 1183–1193 10.1160/TH09-04-0273 [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kozlowski E. O., Pavao M. S., Borsig L. (2011). Ascidian dermatan sulfates attenuate metastasis, inflammation and thrombosis by inhibition of P-selectin. J. Thromb. Haemost. 9, 1807–1815 10.1111/j.1538-7836.2011.04401.x [DOI] [PubMed] [Google Scholar]
  12. Luo L., Wu M., Xu L., Lian W., Xiang J., Lu F., et al. (2013). Comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers. Mar. Drugs 11, 399–417 10.3390/md11020399 [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matsuhiro B., Osorio-Román I. O., Torres R. (2012). Vibrational spectrsocopy characterization and anticoagulant activity of a sulfated polysaccharide from sea cucumber Athyonidium chilensis. Carbohydr. Polym. 88, 959–965 10.1016/j.carbpol.2012.01.052 [DOI] [Google Scholar]
  14. Melo F. R., Pereira M. S., Foguel D., Mourão P. A. (2004). Antithrombin-mediated anticoagulant activity of sulfated polysaccharides: different mechanisms for heparin and sulfated galactans. J. Biol. Chem. 279, 20824–20835 10.1074/jbc.M308688200 [DOI] [PubMed] [Google Scholar]
  15. Mourão P. A. (2004). Use of sulfated fucans as anticoagulant and antithrombotic agents: future perspectives. Curr. Pharm. Des. 10, 967–981 10.2174/1381612043452730 [DOI] [PubMed] [Google Scholar]
  16. Mourão P. A., Perlin A. S. (1987). Structural features of sulfated glycans from the tunic of Styela plicata (Chordata–Tunicata). A unique occurrence of L-galactose in sulfated polysaccharides. Eur. J. Biochem. 166, 431–436 10.1111/j.1432-1033.1987.tb13534.x [DOI] [PubMed] [Google Scholar]
  17. Mourão P. A. S., Pereira M. S., Pavão M. S. G., Mulloy B., Tollefsen D. M., Mowinckel M. C., et al. (1996). Structure and Anticoagulant Activity of a Fucosylated Chondroitin Sulfate from Echinoderm. J. Biol. Chem. 271, 23973–23984 10.1074/jbc.271.39.23973 [DOI] [PubMed] [Google Scholar]
  18. Mulloy B., Ribeiro A.-C., Alves A.-P., Vieira R. P., Mourão P. A. (1994). Sulfated fucans from echinoderms have a regular tetrasaccharide repeating unit defined by specific patterns of sulfation at the O-2 and O-4 positions. J. Biol. Chem. 269, 22113–22123 [PubMed] [Google Scholar]
  19. Nagase H., Enjyoji K.-I., Minamiguchi K., Kitazato K. T., Kitazato K., Saito H., et al. (1995). Depolymerized holothurian glycosaminoglycan with novel anticoagulant actions: antithrombin III- and heparin cofactor II-independent inhibition of factor X activation by factor IXa-factor VIIIa complex and heparin cofactor II-dependent inhibition of thrombin. Blood 85, 1527–1534 [PubMed] [Google Scholar]
  20. Pavão M. S., Aiello K. R., Werneck C. C., Silva L. C., Valente A. P., Mulloy B., et al. (1998). Highly sulfated dermatan sulfates from ascidians. Structure versus anticoagulant activity of these glycosaminoglycans. J. Biol. Chem. 273, 27848–27857 10.1074/jbc.273.43.27848 [DOI] [PubMed] [Google Scholar]
  21. Pereira M. G., Benevides N. M., Melo M. R., Valente A.-P., Melo F. R., Mourão P. A. (2005b). Structure and anticoagulant activity of a sulfated galactan from the red alga, Gelidium crinale. Is there a specific structural requirement for the anticoagulant action? Carbohydr. Res. 340, 2015–2023 10.1016/j.carres.2005.05.018 [DOI] [PubMed] [Google Scholar]
  22. Pereira M. S., Melo F. R., Mourão P. A. (2002). Is there a correlation between structure and anticoagulant action of sulfated galactans and sulfated fucans? Glycobiology 12, 573–580 10.1093/glycob/cwf077 [DOI] [PubMed] [Google Scholar]
  23. Pereira M. S., Mulloy B., Mourão P. A. (1999). Structure and anticoagulant activity of sulfated fucans. Comparison between the regular, repetitive, and linear fucans from echinoderms with the more heterogeneous and branched polymers from brown algae. J. Biol. Chem. 274, 7656–7667 10.1074/jbc.274.12.7656 [DOI] [PubMed] [Google Scholar]
  24. Pereira M. S., Vilela-Silva A.-C., Valente A.-P., Mourão P. A. (2005a). A 2-sulfated, 3-linked alpha-L-galactan is an anticoagulant polysaccharide. Carbohydr. Res. 337, 2231–2238 10.1016/S0008-6215(02)00215-X [DOI] [PubMed] [Google Scholar]
  25. Pomin V. H. (2009). Review: an overview about the structure-function relationship of marine sulfated homopolysaccharides with regular chemical structures. Biopolymers 91, 601–609 10.1002/bip.21200 [DOI] [PubMed] [Google Scholar]
  26. Pomin V. H. (2012a). Fucanomics and galactanomics: marine distribution, medicinal impact, conceptions, and challenges. Mar. Drugs 10, 793–811 10.3390/md10040793 [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pomin V. H. (2012b). Fucanomics and galactanomics: current status in drug discovery, mechanisms of action and role of the well-defined structures. Biochim. Biophys. Acta 1820, 1971–1979 10.1016/j.bbagen.2012.08.022 [DOI] [PubMed] [Google Scholar]
  28. Pomin V. H. (2012c). Structure-function relationship of anticoagulant and antithrombotic well-defined sulfated polysaccharides from marine invertebrates. Adv. Food Nutr. Res. 65, 195–209 10.1016/B978-0-12-416003-3.00012-3 [DOI] [PubMed] [Google Scholar]
  29. Pomin V. H. (2014a). Holothurian fucosylated chondroitin sulfate. Mar. Drugs 12, 232–254 10.3390/md12010232 [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Pomin V. H. (2014b). How to analyze the anticoagulant and antithrombotic mechanism of action in fucanome and galactanome? Glycoconj. J. 31, 89–99 10.1007/s10719-013-9509-3 [DOI] [PubMed] [Google Scholar]
  31. Pomin V. H., Mourão P. A. (2008). Structure, biology, evolution, and medical importance of sulfated fucans and galactans. Glycobiology 18, 1016–1027 10.1093/glycob/cwn085 [DOI] [PubMed] [Google Scholar]
  32. Pomin V. H., Mourão P. A. (2012). Structure versus anticoagulant and antithrombotic actions of marine sulfated polysaccharides. Braz. J. Pharmacog. 22, 921–928 10.1590/S0102-695X2012005000068 [DOI] [Google Scholar]
  33. Pomin V. H., Park Y., Huang R., Heiss C., Sharp J. S., Azadi P., et al. (2012). Exploiting enzyme specificities in digestions of chondroitin sulfates A and C: production of well-defined hexasaccharides. Glycobiology 22, 826–838 10.1093/glycob/cws055 [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Quinderé A. L., Santos G. R., Oliveira S. N., Glauser B. F., Fontes B. P., Queiroz I. N., et al. (2014). Is the antithrombotic effect of sulfated galactans independent of serpin? J. Thromb. Haemost. 12, 43–53 10.1111/jth.12448 [DOI] [PubMed] [Google Scholar]
  35. Santos J. A., Mulloy B., Mourão P. A. (1992). Structural diversity among sulfated alpha-L-galactans from ascidians (tunicates). Studies on the species Ciona intestinalis and Herdmania monus. Eur. J. Biochem. 204, 669–677 10.1111/j.1432-1033.1992.tb16680.x [DOI] [PubMed] [Google Scholar]
  36. Vicente C. P., He L., Pavão M. S., Tollefsen D. M. (2004). Antithrombotic activity of dermatan sulfate in heparin cofactor II-deficient mice. Blood 15, 3965–3970 10.1182/blood-2004-02-0598 [DOI] [PubMed] [Google Scholar]
  37. Vieira R. P., Mourão P. A. (1988). Occurrence of a unique fucose-branched chondroitin sulfate in the body wall of a sea cucumber. J. Biol. Chem. 263, 18176–18183 [PubMed] [Google Scholar]
  38. Vilela-Silva A.-C., Alves A.-P., Valente A.-P., Vacquier V. D., Mourão P. A. (1999). Structure of the sulfated alpha-L-fucan from the egg jelly coat of the sea urchin Strongylocentrotus franciscanus: patterns of preferential 2-O- and 4-O-sulfation determine sperm cell recognition. Glycobiology 9, 227–233 10.1093/glycob/9.9.927 [DOI] [PubMed] [Google Scholar]
  39. Vilela-Silva A.-C., Castro M. O., Valente A.-P., Biermann C. H., Mourão P. A. (2002). Sulfated fucans from the egg jellies of the closely related sea urchins Strongylocentrotus droebachiensis and Strongylocentrotus pallidus ensure species-specific fertilization. J. Biol. Chem., 277, 379–387 10.1074/jbc.M108496200 [DOI] [PubMed] [Google Scholar]
  40. Wu M., Huang R., Wen D., Gao N., He J., Li Z., et al. (2012). Structure and effect of sulfated fucose branches on anticoagulant activity of the fucosylated chondroitin sulfate from sea cucumber Thelenata ananas. Carbohydr. Polym. 87, 862–868 10.1016/j.carbpol.2011.08.082 [DOI] [PubMed] [Google Scholar]
  41. Wu M., Xu S., Zhao J., Kang J., Ding H. (2010). Physicochemical characteristics and anticoagulant activities of low molecular weight fractions by free-radical depolymerization of a fucosylated chondroitin sulphate from sea cucumber Thelenata ananas. Food Chem. 122, 716–723 10.1016/j.foodchem.2010.03.042 [DOI] [Google Scholar]
  42. Ye L., Xu L., Li J. (2012). Preparation and anticoagulant activity of a fucosylated polysaccharide sulfate from a sea cucumber Acaudina molpadioidea. Carbohydr. Polym. 87, 2052–2057 10.1016/j.carbpol.2011.10.014 [DOI] [Google Scholar]
  43. Yoshida H.-I., Minami Y. (1992). Structure of DHG, a depolymerized holothurian glycosaminoglycan from sea cucumber, Stichopus japonicus. Tetrahedron Lett. 33, 4959–4962 10.1016/S0040-4039(00)61245-5 [DOI] [Google Scholar]

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