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. 1995 Aug 15;14(16):3864–3869. doi: 10.1002/j.1460-2075.1995.tb00058.x

The projection structure of microsomal glutathione transferase.

H Hebert 1, I Schmidt-Krey 1, R Morgenstern 1
PMCID: PMC394465  PMID: 7664727

Abstract

Through the use of electron crystallography, it has been possible to obtain high resolution structural information regarding a mammalian protein that spans the lipid bilayer. Two-dimensional crystals of the detoxification enzyme microsomal glutathione transferase were induced by slow detergent removal from a mixture containing low amounts of phospholipid. Images of specimens stabilized in tannin were collected using electron cryomicroscopy. The projection structure at 4 A shows tightly packed trimers of the protein. Each of them contains an inner core of six parallel alpha-helices delineating a central low density region. The helical bundle is partly surrounded by elongated domains.

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Selected References

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