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. 1996 Apr 30;93(9):3886–3891. doi: 10.1073/pnas.93.9.3886

Movement of yeast cortical actin cytoskeleton visualized in vivo.

T Doyle 1, D Botstein 1
PMCID: PMC39454  PMID: 8632984

Abstract

Fusion proteins between the green fluorescent protein (GFP) and the cytoskeleton proteins Act1p (actin), Sac6p (yeast fimbrin homolog), and Abp1p in budding yeast (Saccharomyces cerevisiae) localize to the cortical actin patches. The actin fusions could not function as the sole actin source in yeast, but fusions between the actin-binding proteins Abp1p and Sac6p complement fully the phenotypes associated with their gene deletions. Direct observation in vivo reveals that the actin cortical patches move. Movement of actin patches is constrained to the asymmetric distribution of the patches in growing cells, and this movement is greatly reduced when metabolic inhibitors such as sodium azide are added. Fusion protein-labeled patches are normally distributed during the yeast cell cycle and during mating. In vivo observation made possible the visualization of actin patches during sporulation as well.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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